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The map label for this gene is malZ [C]

Identifier: 159899592

GI number: 159899592

Start: 3880872

End: 3882305

Strand: Direct

Name: malZ [C]

Synonym: Haur_3073

Alternate gene names: 159899592

Gene position: 3880872-3882305 (Clockwise)

Preceding gene: 159899591

Following gene: 159899596

Centisome position: 61.15

GC content: 45.05

Gene sequence:

>1434_bases
ATGACAACCAGCGATTGGCAAACGCCCGATTGGGTCAAACATGCCGTCTTTTATCAGATTTTCCCTGAGCGCTTTGCCAA
TGGTGATCGGACAAATGATCCGGCAAATGCGCAACCTTGGGGTACAAGCCCAACCTTGTATAATTATATGGGCGGCGATC
TACAAGGAATTATCGATAAGCTTGATTATTTAGTGGATTTGGGCATTAATGCGCTGTATCTCAACCCAATTTTTCAAGCC
ACCACCTCACATAAATATAATACCTTCGATTATTTTAAAATCGATCCCCATTTTGGTACGCTAGAAACGTTTAAAACCTT
ATTGAATGAAGCGCATCGACGTGGCATTAAAGTTATTCTCGATGCGGTGTTTAATCATTGCGGTCGCGGCTTTTTTGCCT
TTCACGATGTAATTGAAAATGGTGTGCACTCGCCCTACACCAATTGGTTTCATATCTCACGCTTTCCAATTCATCCCTAT
GAATCGCGCTATGCCGCTAATTATCGCACGTGGTGGGATTTTCGCGAGTTGCCCAAATTCAACACCGATAATCCGGCGGT
ACGCAAATATTTGCTTGATGTAGCTCGCTATTGGATTGAATTGGGTATTGATGGTTGGCGCTTGGATGTGCCAAATGAAA
TTGATGATCATAATTTTTGGCGTGAGTTTCGCACAATTGTCAAAGATATCAATCCTGAAGCCTATATTGTGGGCGAAATT
TGGACTGACGGCTCAGCTTGGCTGCAAGGCGATCAATTTGATGCCGTGATGAATTATCTATTTCGCGATTTATGTACCGA
TTTCTTTGCTAGCTATCGGGTACGTGCCGCTGATTTTGCGGCTGGAATTGACCATTTAATTGTGCGTTATCAGCCCCAAG
TGACCTATGTCCAATTTAATTTGCTTGGTTCACACGATACTGCGCGGTTTTTGAGTGTGGCTGAAGAAGCTGGTAAATGG
GCTTTAGAGCGCATGAAATTGGCGGTTTTGTTCAAATTAATCTTTCCTGGTGCGCCATGTATCTATTATGGCGATGAAAT
TGGCTTGCATGGCGGCAAAGATCCTGATTGTCGGCGTTGTTTCCCGTGGGATCAACCGCAAACCTGGCAGACCGATCTCC
AAGCTTGGACCAAACGCTGGGTTAAGTTTCGCCATGAGCATACAGCCTTGCGCACGGGCCATTATGCGACGCTGTTTGCC
GACAACGATATGAATATTTTTGCTTGTGCCCGTTGGGATGAGCAAAGCCAATTTGTGATTGTGCTGAATAATAACGAAAC
ACCTTGGACACTCGATTTGCCGTTGCATGCCCAATTACCAAGCGTCACTCATTATCGCGATGTGCAAACTGGCGAGTTGT
ATAGCGTGGCCGAGGGTAAAATTCGCGAGGTAGCATTGGCTCCGTGGAAGCATTTGGTATTACAAGCTGAATAG

Upstream 100 bases:

>100_bases
AAGCGTTGATCGGCATGGATACGAATCCATGCCGATTTTGTATTAGGGCTAATCGCTTGTGGTACCATACGCTGCGAAGC
CACGATCAACAGGAGTAGCT

Downstream 100 bases:

>100_bases
TTAAATTGGGCTGCCCAGCGGCGTGCAGCCCAATTATTTAAATCCAGCGGCGAGTTTGGGCTTGATAAGCGCTGTACTGC
TCGCCAAACTTCGCCAGCAT

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTTSDWQTPDWVKHAVFYQIFPERFANGDRTNDPANAQPWGTSPTLYNYMGGDLQGIIDKLDYLVDLGINALYLNPIFQA
TTSHKYNTFDYFKIDPHFGTLETFKTLLNEAHRRGIKVILDAVFNHCGRGFFAFHDVIENGVHSPYTNWFHISRFPIHPY
ESRYAANYRTWWDFRELPKFNTDNPAVRKYLLDVARYWIELGIDGWRLDVPNEIDDHNFWREFRTIVKDINPEAYIVGEI
WTDGSAWLQGDQFDAVMNYLFRDLCTDFFASYRVRAADFAAGIDHLIVRYQPQVTYVQFNLLGSHDTARFLSVAEEAGKW
ALERMKLAVLFKLIFPGAPCIYYGDEIGLHGGKDPDCRRCFPWDQPQTWQTDLQAWTKRWVKFRHEHTALRTGHYATLFA
DNDMNIFACARWDEQSQFVIVLNNNETPWTLDLPLHAQLPSVTHYRDVQTGELYSVAEGKIREVALAPWKHLVLQAE

Sequences:

>Translated_477_residues
MTTSDWQTPDWVKHAVFYQIFPERFANGDRTNDPANAQPWGTSPTLYNYMGGDLQGIIDKLDYLVDLGINALYLNPIFQA
TTSHKYNTFDYFKIDPHFGTLETFKTLLNEAHRRGIKVILDAVFNHCGRGFFAFHDVIENGVHSPYTNWFHISRFPIHPY
ESRYAANYRTWWDFRELPKFNTDNPAVRKYLLDVARYWIELGIDGWRLDVPNEIDDHNFWREFRTIVKDINPEAYIVGEI
WTDGSAWLQGDQFDAVMNYLFRDLCTDFFASYRVRAADFAAGIDHLIVRYQPQVTYVQFNLLGSHDTARFLSVAEEAGKW
ALERMKLAVLFKLIFPGAPCIYYGDEIGLHGGKDPDCRRCFPWDQPQTWQTDLQAWTKRWVKFRHEHTALRTGHYATLFA
DNDMNIFACARWDEQSQFVIVLNNNETPWTLDLPLHAQLPSVTHYRDVQTGELYSVAEGKIREVALAPWKHLVLQAE
>Mature_476_residues
TTSDWQTPDWVKHAVFYQIFPERFANGDRTNDPANAQPWGTSPTLYNYMGGDLQGIIDKLDYLVDLGINALYLNPIFQAT
TSHKYNTFDYFKIDPHFGTLETFKTLLNEAHRRGIKVILDAVFNHCGRGFFAFHDVIENGVHSPYTNWFHISRFPIHPYE
SRYAANYRTWWDFRELPKFNTDNPAVRKYLLDVARYWIELGIDGWRLDVPNEIDDHNFWREFRTIVKDINPEAYIVGEIW
TDGSAWLQGDQFDAVMNYLFRDLCTDFFASYRVRAADFAAGIDHLIVRYQPQVTYVQFNLLGSHDTARFLSVAEEAGKWA
LERMKLAVLFKLIFPGAPCIYYGDEIGLHGGKDPDCRRCFPWDQPQTWQTDLQAWTKRWVKFRHEHTALRTGHYATLFAD
NDMNIFACARWDEQSQFVIVLNNNETPWTLDLPLHAQLPSVTHYRDVQTGELYSVAEGKIREVALAPWKHLVLQAE

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=394, Percent_Identity=25.6345177664975, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786604, Length=468, Percent_Identity=32.6923076923077, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1790687, Length=388, Percent_Identity=28.6082474226804, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789995, Length=492, Percent_Identity=24.5934959349593, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32565753, Length=373, Percent_Identity=21.4477211796247, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=228, Percent_Identity=30.2631578947368, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321726, Length=227, Percent_Identity=28.6343612334802, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=226, Percent_Identity=28.3185840707965, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322021, Length=226, Percent_Identity=28.3185840707965, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324416, Length=226, Percent_Identity=28.3185840707965, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319776, Length=419, Percent_Identity=23.8663484486874, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321731, Length=237, Percent_Identity=27.8481012658228, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24586587, Length=418, Percent_Identity=26.7942583732057, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24583749, Length=410, Percent_Identity=27.5609756097561, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24583747, Length=410, Percent_Identity=27.5609756097561, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24583745, Length=452, Percent_Identity=26.3274336283186, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI221330053, Length=417, Percent_Identity=25.1798561151079, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24586591, Length=219, Percent_Identity=29.6803652968037, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24586599, Length=229, Percent_Identity=31.0043668122271, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24586597, Length=409, Percent_Identity=24.4498777506112, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24586589, Length=399, Percent_Identity=25.31328320802, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24586593, Length=402, Percent_Identity=26.3681592039801, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45549022, Length=557, Percent_Identity=22.6211849192101, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 55721; Mature: 55590

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSDWQTPDWVKHAVFYQIFPERFANGDRTNDPANAQPWGTSPTLYNYMGGDLQGIIDK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHH
LDYLVDLGINALYLNPIFQATTSHKYNTFDYFKIDPHFGTLETFKTLLNEAHRRGIKVIL
HHHHHHCCCCEEEECHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DAVFNHCGRGFFAFHDVIENGVHSPYTNWFHISRFPIHPYESRYAANYRTWWDFRELPKF
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCHHHCCCC
NTDNPAVRKYLLDVARYWIELGIDGWRLDVPNEIDDHNFWREFRTIVKDINPEAYIVGEI
CCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEE
WTDGSAWLQGDQFDAVMNYLFRDLCTDFFASYRVRAADFAAGIDHLIVRYQPQVTYVQFN
ECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCHHHEEEEECCCEEEEEEE
LLGSHDTARFLSVAEEAGKWALERMKLAVLFKLIFPGAPCIYYGDEIGLHGGKDPDCRRC
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCC
FPWDQPQTWQTDLQAWTKRWVKFRHEHTALRTGHYATLFADNDMNIFACARWDEQSQFVI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEEEEEEEECCCCCEEE
VLNNNETPWTLDLPLHAQLPSVTHYRDVQTGELYSVAEGKIREVALAPWKHLVLQAE
EEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCHHHEECCCCCCEEEECC
>Mature Secondary Structure 
TTSDWQTPDWVKHAVFYQIFPERFANGDRTNDPANAQPWGTSPTLYNYMGGDLQGIIDK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHH
LDYLVDLGINALYLNPIFQATTSHKYNTFDYFKIDPHFGTLETFKTLLNEAHRRGIKVIL
HHHHHHCCCCEEEECHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
DAVFNHCGRGFFAFHDVIENGVHSPYTNWFHISRFPIHPYESRYAANYRTWWDFRELPKF
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCHHHCCCC
NTDNPAVRKYLLDVARYWIELGIDGWRLDVPNEIDDHNFWREFRTIVKDINPEAYIVGEI
CCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEE
WTDGSAWLQGDQFDAVMNYLFRDLCTDFFASYRVRAADFAAGIDHLIVRYQPQVTYVQFN
ECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCHHHEEEEECCCEEEEEEE
LLGSHDTARFLSVAEEAGKWALERMKLAVLFKLIFPGAPCIYYGDEIGLHGGKDPDCRRC
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCC
FPWDQPQTWQTDLQAWTKRWVKFRHEHTALRTGHYATLFADNDMNIFACARWDEQSQFVI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEEEEEEEECCCCCEEE
VLNNNETPWTLDLPLHAQLPSVTHYRDVQTGELYSVAEGKIREVALAPWKHLVLQAE
EEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCHHHEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763728 [H]