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The map label for this gene is queA [H]

Identifier: 159899299

GI number: 159899299

Start: 3537936

End: 3538985

Strand: Direct

Name: queA [H]

Synonym: Haur_2780

Alternate gene names: 159899299

Gene position: 3537936-3538985 (Clockwise)

Preceding gene: 159899298

Following gene: 159899300

Centisome position: 55.75

GC content: 52.19

Gene sequence:

>1050_bases
ATGCTTGCAACAATAGATTTTGAATTGCCCGCCGTGCTCGAGGCGGGGGAACCACCGGAAGCACGTGGCTTGGAGCGTGA
TCAAGTGCGCCTGATGGTTTCGCACAGTCGCTCAAATACGATTGAGCATTATCGCTTTGATCAATTGTCGATGATTTTAC
AAGCAGGCGATGTACTGGCAATTAATACTAGTGGCACGCTGAACGCAGCTATTCCCGCCACCGATGCTAGTGGTCAACGT
TTCATGGTGCATCTTTCAACCCAGTTGTTGGCCGAACTATGGCTCGTGGAAGTTCGCTTACCCCATGGCACAAGCAGCCA
GCCATATCGGGCTGCACTGGCTGGGAACAGCCTAAATTTACCATCTGGTGGGCAGATTGATATTCATCGCGGTTATGCAA
ATGATGGATCAGCGATCAATCGTTTGTGGATTGCTAGCCTGCGCTTGCCCCAACCTTTGCCACACTATCTCGCTGAGCAT
GCCAAGCCCATTCATTATCAGTATGTTGCCGCTGATTGGCCCAACAGCTACTATCAAACAGTCTATGCAACTGAACTTGG
CAGCGCCGAAATGCCCTCAGCTGGGCGAGCCTTTTCTGAGCGTTTGCTCACACGCTTGGTGGCGCAGGGCATTCAAATTG
CACCATTATTGCTGCATACAGGCGTGGCCAGTCTTGAAGATCACGAGCCGCCCTATGCCGAATGGTATCGCGTACCAGCA
ACCAGTGCTGATCTGATCAATCGGGCACGCGCTGATCATCGTCGGGTGATCGCGGTTGGCACAACCAGTGTGCGGGCAAT
TGAAAGCACCGTCGATCAACGTGGCTTGGTGCATCCCCAAGCAGGCTGGACAGAATTGGTGATTACGCCAGAGCACGGTT
TGCAGGCGGTCGATGGAATTTTAACGGGCTTGCACGAACCCAAAGCGACCCATTTGGCCATGCTTGCCGCCTTAGCAGGC
TATCAACACCTGGAGTTATGCTACCAAGCCGCGTTAGAACAGCACTATCTTTGGCATGAATTTGGCGATTTACACTTAAT
TTTGCCCTAG

Upstream 100 bases:

>100_bases
TGACCCATAGCCCATAGTCAGTGCGCAGATGCTTTGTGGATCATCAGCCCGATCCCCGCCAAGCGATCCTCGACCCTCTA
TTCATGAAAGTGATCGATCT

Downstream 100 bases:

>100_bases
CCCAATTCATTCGCTAGATTGATCACTATTCAGGAATTGTTTGGCATACTGCTTGAATAGTGATCTTTTTCAAGGATGAT
TAGGCTATTGATGCATCCAA

Product: putative S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLATIDFELPAVLEAGEPPEARGLERDQVRLMVSHSRSNTIEHYRFDQLSMILQAGDVLAINTSGTLNAAIPATDASGQR
FMVHLSTQLLAELWLVEVRLPHGTSSQPYRAALAGNSLNLPSGGQIDIHRGYANDGSAINRLWIASLRLPQPLPHYLAEH
AKPIHYQYVAADWPNSYYQTVYATELGSAEMPSAGRAFSERLLTRLVAQGIQIAPLLLHTGVASLEDHEPPYAEWYRVPA
TSADLINRARADHRRVIAVGTTSVRAIESTVDQRGLVHPQAGWTELVITPEHGLQAVDGILTGLHEPKATHLAMLAALAG
YQHLELCYQAALEQHYLWHEFGDLHLILP

Sequences:

>Translated_349_residues
MLATIDFELPAVLEAGEPPEARGLERDQVRLMVSHSRSNTIEHYRFDQLSMILQAGDVLAINTSGTLNAAIPATDASGQR
FMVHLSTQLLAELWLVEVRLPHGTSSQPYRAALAGNSLNLPSGGQIDIHRGYANDGSAINRLWIASLRLPQPLPHYLAEH
AKPIHYQYVAADWPNSYYQTVYATELGSAEMPSAGRAFSERLLTRLVAQGIQIAPLLLHTGVASLEDHEPPYAEWYRVPA
TSADLINRARADHRRVIAVGTTSVRAIESTVDQRGLVHPQAGWTELVITPEHGLQAVDGILTGLHEPKATHLAMLAALAG
YQHLELCYQAALEQHYLWHEFGDLHLILP
>Mature_349_residues
MLATIDFELPAVLEAGEPPEARGLERDQVRLMVSHSRSNTIEHYRFDQLSMILQAGDVLAINTSGTLNAAIPATDASGQR
FMVHLSTQLLAELWLVEVRLPHGTSSQPYRAALAGNSLNLPSGGQIDIHRGYANDGSAINRLWIASLRLPQPLPHYLAEH
AKPIHYQYVAADWPNSYYQTVYATELGSAEMPSAGRAFSERLLTRLVAQGIQIAPLLLHTGVASLEDHEPPYAEWYRVPA
TSADLINRARADHRRVIAVGTTSVRAIESTVDQRGLVHPQAGWTELVITPEHGLQAVDGILTGLHEPKATHLAMLAALAG
YQHLELCYQAALEQHYLWHEFGDLHLILP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=357, Percent_Identity=30.2521008403361, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38441; Mature: 38441

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATIDFELPAVLEAGEPPEARGLERDQVRLMVSHSRSNTIEHYRFDQLSMILQAGDVLA
CEEEEECCCCHHHCCCCCCHHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEE
INTSGTLNAAIPATDASGQRFMVHLSTQLLAELWLVEVRLPHGTSSQPYRAALAGNSLNL
EECCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHEEEEECCCCCCCCCEEEEECCCCCCC
PSGGQIDIHRGYANDGSAINRLWIASLRLPQPLPHYLAEHAKPIHYQYVAADWPNSYYQT
CCCCEEEEECCCCCCCCHHHHHEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHE
VYATELGSAEMPSAGRAFSERLLTRLVAQGIQIAPLLLHTGVASLEDHEPPYAEWYRVPA
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHCCCCCCCHHHEEECCC
TSADLINRARADHRRVIAVGTTSVRAIESTVDQRGLVHPQAGWTELVITPEHGLQAVDGI
CHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHH
LTGLHEPKATHLAMLAALAGYQHLELCYQAALEQHYLWHEFGDLHLILP
HHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MLATIDFELPAVLEAGEPPEARGLERDQVRLMVSHSRSNTIEHYRFDQLSMILQAGDVLA
CEEEEECCCCHHHCCCCCCHHCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEE
INTSGTLNAAIPATDASGQRFMVHLSTQLLAELWLVEVRLPHGTSSQPYRAALAGNSLNL
EECCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHEEEEECCCCCCCCCEEEEECCCCCCC
PSGGQIDIHRGYANDGSAINRLWIASLRLPQPLPHYLAEHAKPIHYQYVAADWPNSYYQT
CCCCEEEEECCCCCCCCHHHHHEEEECCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHE
VYATELGSAEMPSAGRAFSERLLTRLVAQGIQIAPLLLHTGVASLEDHEPPYAEWYRVPA
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHCCCHHCCCCCCCHHHEEECCC
TSADLINRARADHRRVIAVGTTSVRAIESTVDQRGLVHPQAGWTELVITPEHGLQAVDGI
CHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHH
LTGLHEPKATHLAMLAALAGYQHLELCYQAALEQHYLWHEFGDLHLILP
HHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA