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The map label for this gene is liaS [H]

Identifier: 159899248

GI number: 159899248

Start: 3485642

End: 3487639

Strand: Direct

Name: liaS [H]

Synonym: Haur_2729

Alternate gene names: 159899248

Gene position: 3485642-3487639 (Clockwise)

Preceding gene: 159899247

Following gene: 159899251

Centisome position: 54.92

GC content: 53.3

Gene sequence:

>1998_bases
ATGCGTGTTCTTGATGCCCCAACCAATCAAGCACGACCAACCCCTCAACCACGATTAACCCCAGCCATGAGTTTTGGCTT
GGCAACACTTGGTGTTTTTGTGGGGGTTGGCTGGCTCTGGCTTGCGCCACACTGGCTAGCATTGCTAATCACGCTTGGTT
CATTAAGCCTTTTGCTCGGCCAAGCGTGGGCCAGTATCCAACGCACTGAGCGCGAACGCACGGTTTTAGCTCAAGCACAT
AGTGCCCTCGATAGCGAATATAACAGCATTCAAGCCGATTATGCCACGCTCGAACGGGCCTACGAAGATTTGCAACAATC
GTATCAACGCTTGCAACGGCGGATCGATGAACGCACCGCCGAACGCGATCAATCGACCAATCAGTTGCAAACCTTGCTTG
ATGATACCCGCTCGTATGTCGCCCAACTAACGGCATTGAATGAAGTTTCAGTTGCTTTGAACGCCACGCTTGATCGCGAT
GAGGTGTTGGCTTGTATTTTGCGCCAACTTGAACGGGTGGTTACCTTCGATAGCGCTTCAGTTCAGCAGCTTGAAGATGA
TGAATTGCAAGTAATCGCTGCCCGTGGCATGAATAACGAAGTCTATGATATGCGGATTTCGGTGCCTGATAATGAATTGG
CGCGGAAAGTGGTTGGATCGCCTGCACCAGTGGTTTTGGGCGATGTGCGCAAAGATCCATCGTTTGTGATGCAGCCTGGG
CCAATTCGTTCATGGATTGGGGTGGCCTTGCGGGTGGGCGAACGCACGGTCGGCATTTTGACCGTCGATAGCCATCGCGA
AAATGCCTATACCGCCGATGATGGTCACTTGGTCGCCAATTTTGCCAACCAAGCTGCCTTGGCCTTGCACAACTCACATC
TGTTTGCGGCTGCTGAACGTCGCGCCACCGAAATGGCCTTGCTTTTGGAAATGACCCGCACGGTTGGCTCGACCTTGCAC
TTGCCCGAAGTCTTGCTCCGTGCCGCCGCCGCCATTGGCGAGGCCTTGCATGCTGAAGATGTATTGGTGTTATTGCTTGA
TGAACAGGGCGAGCGTTTGACTCCCCAAGCTGGCGCAATGGGTGATCATAACTACTTGCGCATGCGTTGGGCCAGCCCAA
CCTCGTTGAGTAACGAGCCAACTTTAGCCAAAGTGATCAAACAAGGCTGCGCCAGAGTAATTCATGCGGTTTCGCCGACG
ATTCCTTACCAAACCTTGTTGGCCTTGCCATTGAGCATCAAAGAGCAAGTTTTAGGGGTTGTCTTGGTCGCCACACCTGA
TCGCGATGCCCCATTTGGGCCACAGCAATTGGTTTTAGCTGAAGGTTTAGCCACCTCAGCCGCCATCGCGATCGAACAAG
CACGTTTGTATGATCAAGCTCGACGCGCGGCTCAAGCTGAGGAGCGTTCACGTTTGGCGCGTGAATTGCACGATTCGGTC
ACCCAGACCTTGTTTAGCATGACCTTGACGGCTGAAGCTGCCCGTGCCCAAGTTGAGCGCAACCCAGTTCGGGCGGCCAC
TCAAATTGATCGCTTGAAGGCCGCAGCCCATCAATCACTAGGCGAAATGCGTGAACTCTTGCTGCAATTACGCCCAACGC
CGTTGCAAGAACATGGCATTATCAAATCGCTGCGCGACCATATTGCCAGCTTGAATGCTCAAGAAGTTGCCATTAATTTG
GAGTGTGAGGGCGAGGATTCGGTACTTAGCCCAGCCAATGCTGCTGGCTTATATCGAATTGCCCAAGAAGCGATTGCCAA
TGCGCTGCGCCATGCCCAAGCAACTAATGTACGAGTGAGCTTCAGCTTTGCCAGCAAATCGACGACCTTGATGGTGAAAG
ATAATGGCTGTGGCTTCGACCCCGATTTATTGGAGCGCAGTGGCCGCCATTTGGGGCTAACCAGTATGGCCGAGCGTGCC
GGAGAACTGGGTGGCACGCTCGATGTGCAAAGTGTCGTTGGCGAAGGTACCTGCTTAACCGTGCACCTGCATGGGTAA

Upstream 100 bases:

>100_bases
GTAATTGATAATAGCGGTTCGCTCGCGGAAACTCGCCGCCAAGTTGAACAAGCATTTTTGGCGATTCAACGCCATGATTA
AACCAAGTAAGGAGCCAGTT

Downstream 100 bases:

>100_bases
GCTTGTTGCATTCGTACCAATCGCAAAGGCCTAAGCAAATTGATTGATTGCCTAGCCTTGTTAATCGCACTTTAGGGGGT
GCAGGGGGATGAACCTATAG

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MRVLDAPTNQARPTPQPRLTPAMSFGLATLGVFVGVGWLWLAPHWLALLITLGSLSLLLGQAWASIQRTERERTVLAQAH
SALDSEYNSIQADYATLERAYEDLQQSYQRLQRRIDERTAERDQSTNQLQTLLDDTRSYVAQLTALNEVSVALNATLDRD
EVLACILRQLERVVTFDSASVQQLEDDELQVIAARGMNNEVYDMRISVPDNELARKVVGSPAPVVLGDVRKDPSFVMQPG
PIRSWIGVALRVGERTVGILTVDSHRENAYTADDGHLVANFANQAALALHNSHLFAAAERRATEMALLLEMTRTVGSTLH
LPEVLLRAAAAIGEALHAEDVLVLLLDEQGERLTPQAGAMGDHNYLRMRWASPTSLSNEPTLAKVIKQGCARVIHAVSPT
IPYQTLLALPLSIKEQVLGVVLVATPDRDAPFGPQQLVLAEGLATSAAIAIEQARLYDQARRAAQAEERSRLARELHDSV
TQTLFSMTLTAEAARAQVERNPVRAATQIDRLKAAAHQSLGEMRELLLQLRPTPLQEHGIIKSLRDHIASLNAQEVAINL
ECEGEDSVLSPANAAGLYRIAQEAIANALRHAQATNVRVSFSFASKSTTLMVKDNGCGFDPDLLERSGRHLGLTSMAERA
GELGGTLDVQSVVGEGTCLTVHLHG

Sequences:

>Translated_665_residues
MRVLDAPTNQARPTPQPRLTPAMSFGLATLGVFVGVGWLWLAPHWLALLITLGSLSLLLGQAWASIQRTERERTVLAQAH
SALDSEYNSIQADYATLERAYEDLQQSYQRLQRRIDERTAERDQSTNQLQTLLDDTRSYVAQLTALNEVSVALNATLDRD
EVLACILRQLERVVTFDSASVQQLEDDELQVIAARGMNNEVYDMRISVPDNELARKVVGSPAPVVLGDVRKDPSFVMQPG
PIRSWIGVALRVGERTVGILTVDSHRENAYTADDGHLVANFANQAALALHNSHLFAAAERRATEMALLLEMTRTVGSTLH
LPEVLLRAAAAIGEALHAEDVLVLLLDEQGERLTPQAGAMGDHNYLRMRWASPTSLSNEPTLAKVIKQGCARVIHAVSPT
IPYQTLLALPLSIKEQVLGVVLVATPDRDAPFGPQQLVLAEGLATSAAIAIEQARLYDQARRAAQAEERSRLARELHDSV
TQTLFSMTLTAEAARAQVERNPVRAATQIDRLKAAAHQSLGEMRELLLQLRPTPLQEHGIIKSLRDHIASLNAQEVAINL
ECEGEDSVLSPANAAGLYRIAQEAIANALRHAQATNVRVSFSFASKSTTLMVKDNGCGFDPDLLERSGRHLGLTSMAERA
GELGGTLDVQSVVGEGTCLTVHLHG
>Mature_665_residues
MRVLDAPTNQARPTPQPRLTPAMSFGLATLGVFVGVGWLWLAPHWLALLITLGSLSLLLGQAWASIQRTERERTVLAQAH
SALDSEYNSIQADYATLERAYEDLQQSYQRLQRRIDERTAERDQSTNQLQTLLDDTRSYVAQLTALNEVSVALNATLDRD
EVLACILRQLERVVTFDSASVQQLEDDELQVIAARGMNNEVYDMRISVPDNELARKVVGSPAPVVLGDVRKDPSFVMQPG
PIRSWIGVALRVGERTVGILTVDSHRENAYTADDGHLVANFANQAALALHNSHLFAAAERRATEMALLLEMTRTVGSTLH
LPEVLLRAAAAIGEALHAEDVLVLLLDEQGERLTPQAGAMGDHNYLRMRWASPTSLSNEPTLAKVIKQGCARVIHAVSPT
IPYQTLLALPLSIKEQVLGVVLVATPDRDAPFGPQQLVLAEGLATSAAIAIEQARLYDQARRAAQAEERSRLARELHDSV
TQTLFSMTLTAEAARAQVERNPVRAATQIDRLKAAAHQSLGEMRELLLQLRPTPLQEHGIIKSLRDHIASLNAQEVAINL
ECEGEDSVLSPANAAGLYRIAQEAIANALRHAQATNVRVSFSFASKSTTLMVKDNGCGFDPDLLERSGRHLGLTSMAERA
GELGGTLDVQSVVGEGTCLTVHLHG

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=271, Percent_Identity=29.1512915129151, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787474, Length=278, Percent_Identity=29.8561151079137, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI87082311, Length=208, Percent_Identity=29.3269230769231, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72534; Mature: 72534

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLDAPTNQARPTPQPRLTPAMSFGLATLGVFVGVGWLWLAPHWLALLITLGSLSLLLG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAWASIQRTERERTVLAQAHSALDSEYNSIQADYATLERAYEDLQQSYQRLQRRIDERTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDQSTNQLQTLLDDTRSYVAQLTALNEVSVALNATLDRDEVLACILRQLERVVTFDSAS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCH
VQQLEDDELQVIAARGMNNEVYDMRISVPDNELARKVVGSPAPVVLGDVRKDPSFVMQPG
HHHCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCCEECCC
PIRSWIGVALRVGERTVGILTVDSHRENAYTADDGHLVANFANQAALALHNSHLFAAAER
CHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEHHHCCCEEEEECCHHHHHHHH
RATEMALLLEMTRTVGSTLHLPEVLLRAAAAIGEALHAEDVLVLLLDEQGERLTPQAGAM
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GDHNYLRMRWASPTSLSNEPTLAKVIKQGCARVIHAVSPTIPYQTLLALPLSIKEQVLGV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHCEE
VLVATPDRDAPFGPQQLVLAEGLATSAAIAIEQARLYDQARRAAQAEERSRLARELHDSV
EEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQTLFSMTLTAEAARAQVERNPVRAATQIDRLKAAAHQSLGEMRELLLQLRPTPLQEHGI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IKSLRDHIASLNAQEVAINLECEGEDSVLSPANAAGLYRIAQEAIANALRHAQATNVRVS
HHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
FSFASKSTTLMVKDNGCGFDPDLLERSGRHLGLTSMAERAGELGGTLDVQSVVGEGTCLT
EEECCCCEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEHHHHHCCCEEEE
VHLHG
EEECC
>Mature Secondary Structure
MRVLDAPTNQARPTPQPRLTPAMSFGLATLGVFVGVGWLWLAPHWLALLITLGSLSLLLG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAWASIQRTERERTVLAQAHSALDSEYNSIQADYATLERAYEDLQQSYQRLQRRIDERTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERDQSTNQLQTLLDDTRSYVAQLTALNEVSVALNATLDRDEVLACILRQLERVVTFDSAS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCCCH
VQQLEDDELQVIAARGMNNEVYDMRISVPDNELARKVVGSPAPVVLGDVRKDPSFVMQPG
HHHCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEECCCCCCCCEECCC
PIRSWIGVALRVGERTVGILTVDSHRENAYTADDGHLVANFANQAALALHNSHLFAAAER
CHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEHHHCCCEEEEECCHHHHHHHH
RATEMALLLEMTRTVGSTLHLPEVLLRAAAAIGEALHAEDVLVLLLDEQGERLTPQAGAM
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
GDHNYLRMRWASPTSLSNEPTLAKVIKQGCARVIHAVSPTIPYQTLLALPLSIKEQVLGV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHCEE
VLVATPDRDAPFGPQQLVLAEGLATSAAIAIEQARLYDQARRAAQAEERSRLARELHDSV
EEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQTLFSMTLTAEAARAQVERNPVRAATQIDRLKAAAHQSLGEMRELLLQLRPTPLQEHGI
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IKSLRDHIASLNAQEVAINLECEGEDSVLSPANAAGLYRIAQEAIANALRHAQATNVRVS
HHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEE
FSFASKSTTLMVKDNGCGFDPDLLERSGRHLGLTSMAERAGELGGTLDVQSVVGEGTCLT
EEECCCCEEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCEEHHHHHCCCEEEE
VHLHG
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]