Click here to switch to the map view.

The map label for this gene is galE [C]

Identifier: 159898196

GI number: 159898196

Start: 1938874

End: 1939923

Strand: Reverse

Name: galE [C]

Synonym: Haur_1672

Alternate gene names: 159898196

Gene position: 1939923-1938874 (Counterclockwise)

Preceding gene: 159898197

Following gene: 159898195

Centisome position: 30.57

GC content: 51.81

Gene sequence:

>1050_bases
GTGGAACGACGCACAGTGCTGATCACAGGGGCTGCTGGTCGCTGGGGACGGCGATTAGCCCGCCAATTGGCGAAATTTCC
GATTGTGCGCGTGATTGGCCTTGATATTACTCCACCAGAACGCCCAATTGATGGCGTTGAGTATATTCAGGCCGATTTAC
GCAACCCATTGTTGGTCGAATTGCTTCGCACCGAGAAGGTCGAAGGCATTTTACACATGGTCTTTACCTCTGGTTTATCT
GGAGGTCGCGGCCTGCTTTCGCCCGAAGATGCCTTTCAAACCAATGTGATGGGCACAATGGAGGTTTTGGCGGCGGCAAA
TGCTGCTGACGTTAAACAGGTGGTAATCAAAAGCACAACCATGGTTTATGGGGCGCACGTTGATAACCCCTTGTATTTGC
ATGAAAATGCGCCGCTACGTGGCACTGATGGCAAGCCACTCTTGCGCCATCGCTATGAAATCGAGCGTTTTATCGCTGAT
TTTCGGCGTAATCACGCCATGAATATTGCGGTTTTGCGCTTTCCAGGCATTGTTGGAGCCAGCGGCGCATCATCAACCAT
GCGCTACCTAGGTCAACCAACCCCACCAACCCTGCTTGGCTTTGACCCATTGATGCAAATTGTGCATGAGGCCGATGTGA
TTGGCTCCTTGCTACATGCCTTGGTCGCTGAAGCCAATGGGGTTTTTAATGTTGCTGCCGATGGTGTAATGCCGCTGAGC
CAAATGATTCGGCGGGTTGGTCGCCAACCGTTGCCAATTTTCCACCCCTTGGCCTATGCCGCTGGACGATTCGGCTTTGT
ACGCCACAATCGGCTAGCCAACCAAATTATGCCATTTGAATTAGATTATTTGCGCTTTCCTTGGGTTGCCGATACCACGG
CCATGCGCGGCGAATTAAATTACGAGCCACGTCACACCGCCGAGGAAGCGCTCGATGCCTTCCGTAGCTCGTTGCGCTTG
AAAAGCTACAGCGATTTATTGCCCAAACGCAAAGCCTACGATGGAATTCAAGCCTTTTTAAATCGTCGCCGTCAGGAGGA
TGAAGCATGA

Upstream 100 bases:

>100_bases
CACGTTTTTTTGCATAGAGCTTCACCCACGTTCGCTGCCACGCTCTATGTGCTCACCACGGTTGTGCTCGCATTTGGCTT
TTTTTGGATAAGGAGCTTTT

Downstream 100 bases:

>100_bases
GCGATCAACCCCAAAAACGTCGCCGTCGCGCTGCTGGCGGTGCTGATGAAGCCGAACAAACAATTCCAGTTGTTGATGCA
AGCACTGAAGAAACTGCGCT

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MERRTVLITGAAGRWGRRLARQLAKFPIVRVIGLDITPPERPIDGVEYIQADLRNPLLVELLRTEKVEGILHMVFTSGLS
GGRGLLSPEDAFQTNVMGTMEVLAAANAADVKQVVIKSTTMVYGAHVDNPLYLHENAPLRGTDGKPLLRHRYEIERFIAD
FRRNHAMNIAVLRFPGIVGASGASSTMRYLGQPTPPTLLGFDPLMQIVHEADVIGSLLHALVAEANGVFNVAADGVMPLS
QMIRRVGRQPLPIFHPLAYAAGRFGFVRHNRLANQIMPFELDYLRFPWVADTTAMRGELNYEPRHTAEEALDAFRSSLRL
KSYSDLLPKRKAYDGIQAFLNRRRQEDEA

Sequences:

>Translated_349_residues
MERRTVLITGAAGRWGRRLARQLAKFPIVRVIGLDITPPERPIDGVEYIQADLRNPLLVELLRTEKVEGILHMVFTSGLS
GGRGLLSPEDAFQTNVMGTMEVLAAANAADVKQVVIKSTTMVYGAHVDNPLYLHENAPLRGTDGKPLLRHRYEIERFIAD
FRRNHAMNIAVLRFPGIVGASGASSTMRYLGQPTPPTLLGFDPLMQIVHEADVIGSLLHALVAEANGVFNVAADGVMPLS
QMIRRVGRQPLPIFHPLAYAAGRFGFVRHNRLANQIMPFELDYLRFPWVADTTAMRGELNYEPRHTAEEALDAFRSSLRL
KSYSDLLPKRKAYDGIQAFLNRRRQEDEA
>Mature_349_residues
MERRTVLITGAAGRWGRRLARQLAKFPIVRVIGLDITPPERPIDGVEYIQADLRNPLLVELLRTEKVEGILHMVFTSGLS
GGRGLLSPEDAFQTNVMGTMEVLAAANAADVKQVVIKSTTMVYGAHVDNPLYLHENAPLRGTDGKPLLRHRYEIERFIAD
FRRNHAMNIAVLRFPGIVGASGASSTMRYLGQPTPPTLLGFDPLMQIVHEADVIGSLLHALVAEANGVFNVAADGVMPLS
QMIRRVGRQPLPIFHPLAYAAGRFGFVRHNRLANQIMPFELDYLRFPWVADTTAMRGELNYEPRHTAEEALDAFRSSLRL
KSYSDLLPKRKAYDGIQAFLNRRRQEDEA

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 38971; Mature: 38971

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRTVLITGAAGRWGRRLARQLAKFPIVRVIGLDITPPERPIDGVEYIQADLRNPLLVE
CCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHH
LLRTEKVEGILHMVFTSGLSGGRGLLSPEDAFQTNVMGTMEVLAAANAADVKQVVIKSTT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MVYGAHVDNPLYLHENAPLRGTDGKPLLRHRYEIERFIADFRRNHAMNIAVLRFPGIVGA
EEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SGASSTMRYLGQPTPPTLLGFDPLMQIVHEADVIGSLLHALVAEANGVFNVAADGVMPLS
CCHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHH
QMIRRVGRQPLPIFHPLAYAAGRFGFVRHNRLANQIMPFELDYLRFPWVADTTAMRGELN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEECCCCCCCHHHCCCCC
YEPRHTAEEALDAFRSSLRLKSYSDLLPKRKAYDGIQAFLNRRRQEDEA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MERRTVLITGAAGRWGRRLARQLAKFPIVRVIGLDITPPERPIDGVEYIQADLRNPLLVE
CCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHH
LLRTEKVEGILHMVFTSGLSGGRGLLSPEDAFQTNVMGTMEVLAAANAADVKQVVIKSTT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MVYGAHVDNPLYLHENAPLRGTDGKPLLRHRYEIERFIADFRRNHAMNIAVLRFPGIVGA
EEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SGASSTMRYLGQPTPPTLLGFDPLMQIVHEADVIGSLLHALVAEANGVFNVAADGVMPLS
CCHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHH
QMIRRVGRQPLPIFHPLAYAAGRFGFVRHNRLANQIMPFELDYLRFPWVADTTAMRGELN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCEECCCCCCCHHHCCCCC
YEPRHTAEEALDAFRSSLRLKSYSDLLPKRKAYDGIQAFLNRRRQEDEA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]