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The map label for this gene is ycjG [C]

Identifier: 159897643

GI number: 159897643

Start: 1275907

End: 1276977

Strand: Direct

Name: ycjG [C]

Synonym: Haur_1114

Alternate gene names: 159897643

Gene position: 1275907-1276977 (Clockwise)

Preceding gene: 159897642

Following gene: 159897644

Centisome position: 20.1

GC content: 53.78

Gene sequence:

>1071_bases
ATGCCGACGACGATTCAGGCCATCAGTGCCGAAGCGATTAATTTGCCTTTGACCGAGCCGTTTGCAATTGCCAGCGGGGC
GCAAGCGGTCGCTGCCAATGTTTTAGTCAAAGTTCAGTTGGCTGATGGCACGCTTGGCTTAGGCGAGGCGGCTCCTTTTC
CCGCTGTCAGCGGCGAAACCCAGACTGGCACAAGCGCTGCGATTGAGCGCTTGCAAAGCCATTTGCTGGGAGCCGATGTG
CGTGGATGGCGCAAACTAGCGGCGATGCTGGATCATGCTGAACATGAGGCGGCTGCCGCTCGTTGTGGCCTTGAAATGGC
CATGCTTGATGCGCTAACTCGCCATTATCACATGCCATTACACGTATTTTTTGGCGGCGTGAGTAAGCAACTTGAAACTG
ACATGACGATTACCGCAGGTGATGAGGTGCATGCGGCGGCCTCAGCCAAGGCCATTCTTGCCCGTGGCATCAAATCGATC
AAAGTTAAAACGGCTGGGGTTGATGTGGCCTATGATTTGGCGCGACTGCGGGCCATTCATCAAGCAGCGCCCACCGCACC
ATTAATTGTTGATGGCAATTGCGGCTATGATGTCGAACGGGCACTAGCTTTTTGCGCTGCTTGTAAGGCCGAGTCAATTC
CGATGGTGTTGTTTGAGCAACCGTTGCCGCGCGAGGATTGGGCAGGCATGGCCCAGGTTACGGCCCAATCAGGCTTTGCA
GTTGCTGCTGATGAATCTGCGCGTTCAGCTCACGATGTGCTACGGATCGCCCGCGAAGGTACAGCCTCGGTGATTAACAT
TAAATTGATGAAGGCTGGCGTAGCTGAAGGCTTGAAGATGATTGCAATTGCCCAGGCTGCTGGCTTGGGATTGATGATTG
GCGGCATGGTTGAAAGTATTTTGGCCATGAGCTTTTCGGCCAATCTGGCGGCTGGTAATGGCGGCTTCGATTTTATCGAT
CTTGATACGCCATTATTTATTGCCGAGCATCCCTTTATTGGTGGCTTTGCTCAAACTGGCGGAACCCTGCAATTAGCCGA
TGTTGCTGGCCATGGCGTGAATCTGGCCTAA

Upstream 100 bases:

>100_bases
CAATCGGCCATCGCCGCACCAACGAAGTTCAAGCATGGGTTGCCCAGCTAACGCCGCTATAATACAAGCATCAACAGCTT
TGTAGGATAGGAGCTTTGCT

Downstream 100 bases:

>100_bases
GCTAACCCTCTATTCTAGCCCGATCATGGCAATTTATGCTATTTTCGGGCTAGCTTTTTGCCCCTACTTGCACTAGACTT
GCTGTAACCAAACCACATTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPTTIQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGETQTGTSAAIERLQSHLLGADV
RGWRKLAAMLDHAEHEAAAARCGLEMAMLDALTRHYHMPLHVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSI
KVKTAGVDVAYDLARLRAIHQAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVTAQSGFA
VAADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGMVESILAMSFSANLAAGNGGFDFID
LDTPLFIAEHPFIGGFAQTGGTLQLADVAGHGVNLA

Sequences:

>Translated_356_residues
MPTTIQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGETQTGTSAAIERLQSHLLGADV
RGWRKLAAMLDHAEHEAAAARCGLEMAMLDALTRHYHMPLHVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSI
KVKTAGVDVAYDLARLRAIHQAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVTAQSGFA
VAADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGMVESILAMSFSANLAAGNGGFDFID
LDTPLFIAEHPFIGGFAQTGGTLQLADVAGHGVNLA
>Mature_355_residues
PTTIQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGETQTGTSAAIERLQSHLLGADVR
GWRKLAAMLDHAEHEAAAARCGLEMAMLDALTRHYHMPLHVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSIK
VKTAGVDVAYDLARLRAIHQAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVTAQSGFAV
AADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGMVESILAMSFSANLAAGNGGFDFIDL
DTPLFIAEHPFIGGFAQTGGTLQLADVAGHGVNLA

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of a broad range of other

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=316, Percent_Identity=31.0126582278481, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 36785; Mature: 36654

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTIQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGET
CCCCHHECCHHHCCCCCCCCHHHCCCHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
QTGTSAAIERLQSHLLGADVRGWRKLAAMLDHAEHEAAAARCGLEMAMLDALTRHYHMPL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCE
HVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSIKVKTAGVDVAYDLARLRAIH
EEEECCHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHEEEEECCCHHHHHHHHHHHHH
QAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVTAQSGFA
HHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHCCHHHHHHCCCEE
VAADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGMVESI
EEECCHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAMSFSANLAAGNGGFDFIDLDTPLFIAEHPFIGGFAQTGGTLQLADVAGHGVNLA
HHHHHCCCEECCCCCEEEEECCCCEEEECCCCCCCHHCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
PTTIQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGET
CCCHHECCHHHCCCCCCCCHHHCCCHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCC
QTGTSAAIERLQSHLLGADVRGWRKLAAMLDHAEHEAAAARCGLEMAMLDALTRHYHMPL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCE
HVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSIKVKTAGVDVAYDLARLRAIH
EEEECCHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHEEEEECCCHHHHHHHHHHHHH
QAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVTAQSGFA
HHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHCCHHHHHHCCCEE
VAADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGMVESI
EEECCHHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAMSFSANLAAGNGGFDFIDLDTPLFIAEHPFIGGFAQTGGTLQLADVAGHGVNLA
HHHHHCCCEECCCCCEEEEECCCCEEEECCCCCCCHHCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]