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The map label for this gene is fixL [H]

Identifier: 159897348

GI number: 159897348

Start: 926690

End: 927871

Strand: Reverse

Name: fixL [H]

Synonym: Haur_0819

Alternate gene names: 159897348

Gene position: 927871-926690 (Counterclockwise)

Preceding gene: 159897349

Following gene: 159897347

Centisome position: 14.62

GC content: 51.02

Gene sequence:

>1182_bases
ATGCTGAGTCGTGAACAGCTTGAAACAGAAAATGCTAGCCTTAAACGTGAAGTTGAGTTATTACGCCATCAATTGGCAAC
CCAACCAGCCGAGCCGCTTTTTCAAAGTTTGTTAGAGGCGGCTCCTGATGGCGTGGTGATTGTCAATGCCCACGGCATTA
TTAGCGTGGTCAATGCCCAAGCCGAGGCTATTTTTGGTTATGATCGTGAATCGATGGTTGGTCAACCAATCGAATTGTTG
ATTCCCGAGCGCGTGCGTGGCGTGCATGTGGCCTATCGCAGTAATTACACCCGCGAGCCACGCACCCGCCCAATGGGCAT
TGGCTCAGATTTATTGGCGCGGCGCAGTGATGGCACTGAGTTTTACACCGAAATTAGCCTTAGCCCATTGAAAACCGCTG
ATCAATTGTTAATTATTGCAGTGGTACGCGATGTGACCGAGCAAAAGAAACTTGCCAAAGAGCATGAACATCGCTTAACC
CAACGGGTCAGTGCCGCTGAAGCCGCCTTGTTACAAGGCGCACGCTTGGCAGCGGTAGGCCAGCTTTCAGCCTCGATTGC
CCACGAAATTAACAATCCGCTGTATGCGGCCCGCAATGCCTTGCTATTGCTCGAAGAAGATTTGCCCGAAGAAGTGCGCG
AATCAACTGTGATGCAATTAGCCCGCGATCAATTAACCCGCATCGCCGGAATTATCGAACGCATGCGTGATTTTTATCGC
CCGATGCGCGGCGAGATGGCTCCCACCGATCTGAACGCCGTGATCGAAGAAACCTTAGCGCTAGCTGGCTTGCACGCCCG
CCATAACAACACCATGATCACATTTACGCCTGATATCCATTTGCCAGCGGTGGTTTGCAACGCCGACCAACTACGCCAAG
TCTTTTTGAACTTGGTGTTGAACGCATTGGATGCGATGACCAACGGTGGTACATTAACCGTCACAACCGAAGCTGCCACC
AGCTTTGTTACAATAGAAATTTGCGATACGGGAACAGGCATTCCTGATGATATTCGGGCACACCTGTTTGAGCCATTTTT
TACCAACAAACCCAATGGAACCGGTCTTGGTTTGCCGATTAGTGCCCATATTGTGACCCAGCATGGTGGCCAAATCGAGG
TTGACAGCACCGTTGGGGTTGGCTCGACCTTCCGTGTTGTGCTACCCTACCAACCTAACTAG

Upstream 100 bases:

>100_bases
TGGTTTCAACCCTCGCGCCCTTCGTGGCCTTCGTGGATCAACCCTGACCCCTCGCCTGCAAATATGCTATGATTGCCAAT
CATTCAAGGGAGTTTTTGGT

Downstream 100 bases:

>100_bases
GAGTATTCTTCGATGAGTCAAGCTTCGATTTTATTAGTTGATGATGAATTTCCCATTCGCGCCACCCTCGGCGATTTACT
GCGGCGACGGGGCTACGATG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLSREQLETENASLKREVELLRHQLATQPAEPLFQSLLEAAPDGVVIVNAHGIISVVNAQAEAIFGYDRESMVGQPIELL
IPERVRGVHVAYRSNYTREPRTRPMGIGSDLLARRSDGTEFYTEISLSPLKTADQLLIIAVVRDVTEQKKLAKEHEHRLT
QRVSAAEAALLQGARLAAVGQLSASIAHEINNPLYAARNALLLLEEDLPEEVRESTVMQLARDQLTRIAGIIERMRDFYR
PMRGEMAPTDLNAVIEETLALAGLHARHNNTMITFTPDIHLPAVVCNADQLRQVFLNLVLNALDAMTNGGTLTVTTEAAT
SFVTIEICDTGTGIPDDIRAHLFEPFFTNKPNGTGLGLPISAHIVTQHGGQIEVDSTVGVGSTFRVVLPYQPN

Sequences:

>Translated_393_residues
MLSREQLETENASLKREVELLRHQLATQPAEPLFQSLLEAAPDGVVIVNAHGIISVVNAQAEAIFGYDRESMVGQPIELL
IPERVRGVHVAYRSNYTREPRTRPMGIGSDLLARRSDGTEFYTEISLSPLKTADQLLIIAVVRDVTEQKKLAKEHEHRLT
QRVSAAEAALLQGARLAAVGQLSASIAHEINNPLYAARNALLLLEEDLPEEVRESTVMQLARDQLTRIAGIIERMRDFYR
PMRGEMAPTDLNAVIEETLALAGLHARHNNTMITFTPDIHLPAVVCNADQLRQVFLNLVLNALDAMTNGGTLTVTTEAAT
SFVTIEICDTGTGIPDDIRAHLFEPFFTNKPNGTGLGLPISAHIVTQHGGQIEVDSTVGVGSTFRVVLPYQPN
>Mature_393_residues
MLSREQLETENASLKREVELLRHQLATQPAEPLFQSLLEAAPDGVVIVNAHGIISVVNAQAEAIFGYDRESMVGQPIELL
IPERVRGVHVAYRSNYTREPRTRPMGIGSDLLARRSDGTEFYTEISLSPLKTADQLLIIAVVRDVTEQKKLAKEHEHRLT
QRVSAAEAALLQGARLAAVGQLSASIAHEINNPLYAARNALLLLEEDLPEEVRESTVMQLARDQLTRIAGIIERMRDFYR
PMRGEMAPTDLNAVIEETLALAGLHARHNNTMITFTPDIHLPAVVCNADQLRQVFLNLVLNALDAMTNGGTLTVTTEAAT
SFVTIEICDTGTGIPDDIRAHLFEPFFTNKPNGTGLGLPISAHIVTQHGGQIEVDSTVGVGSTFRVVLPYQPN

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=30.1630434782609, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=35.3488372093023, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790300, Length=231, Percent_Identity=32.4675324675325, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786600, Length=370, Percent_Identity=27.2972972972973, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI145693157, Length=356, Percent_Identity=28.0898876404494, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994928, Length=384, Percent_Identity=23.6979166666667, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082128, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789808, Length=217, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081816, Length=385, Percent_Identity=25.974025974026, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081921, Length=121, Percent_Identity=28.9256198347107, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=243, Percent_Identity=27.9835390946502, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788393, Length=224, Percent_Identity=27.2321428571429, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1790861, Length=203, Percent_Identity=26.1083743842365, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1788713, Length=375, Percent_Identity=22.1333333333333, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43124; Mature: 43124

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSREQLETENASLKREVELLRHQLATQPAEPLFQSLLEAAPDGVVIVNAHGIISVVNAQ
CCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCC
AEAIFGYDRESMVGQPIELLIPERVRGVHVAYRSNYTREPRTRPMGIGSDLLARRSDGTE
HHHHCCCCHHHHCCCCHHHCCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCE
FYTEISLSPLKTADQLLIIAVVRDVTEQKKLAKEHEHRLTQRVSAAEAALLQGARLAAVG
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QLSASIAHEINNPLYAARNALLLLEEDLPEEVRESTVMQLARDQLTRIAGIIERMRDFYR
HHHHHHHHHHCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMRGEMAPTDLNAVIEETLALAGLHARHNNTMITFTPDIHLPAVVCNADQLRQVFLNLVL
HHCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCEEEECHHHHHHHHHHHHH
NALDAMTNGGTLTVTTEAATSFVTIEICDTGTGIPDDIRAHLFEPFFTNKPNGTGLGLPI
HHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCCCCCCEE
SAHIVTQHGGQIEVDSTVGVGSTFRVVLPYQPN
EEEEEECCCCEEEECCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MLSREQLETENASLKREVELLRHQLATQPAEPLFQSLLEAAPDGVVIVNAHGIISVVNAQ
CCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCEEEEECCC
AEAIFGYDRESMVGQPIELLIPERVRGVHVAYRSNYTREPRTRPMGIGSDLLARRSDGTE
HHHHCCCCHHHHCCCCHHHCCCHHHCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCE
FYTEISLSPLKTADQLLIIAVVRDVTEQKKLAKEHEHRLTQRVSAAEAALLQGARLAAVG
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QLSASIAHEINNPLYAARNALLLLEEDLPEEVRESTVMQLARDQLTRIAGIIERMRDFYR
HHHHHHHHHHCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PMRGEMAPTDLNAVIEETLALAGLHARHNNTMITFTPDIHLPAVVCNADQLRQVFLNLVL
HHCCCCCCCHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCEEEECHHHHHHHHHHHHH
NALDAMTNGGTLTVTTEAATSFVTIEICDTGTGIPDDIRAHLFEPFFTNKPNGTGLGLPI
HHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCCCCCCEE
SAHIVTQHGGQIEVDSTVGVGSTFRVVLPYQPN
EEEEEECCCCEEEECCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]