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The map label for this gene is hgiDIIM [H]

Identifier: 159897057

GI number: 159897057

Start: 614213

End: 615277

Strand: Direct

Name: hgiDIIM [H]

Synonym: Haur_0528

Alternate gene names: 159897057

Gene position: 614213-615277 (Clockwise)

Preceding gene: 159897056

Following gene: 159897058

Centisome position: 9.68

GC content: 43.57

Gene sequence:

>1065_bases
ATGGTTGGCGCAGTCATTGATCTATTCTGTGGAGTTGGTGGGCTAACCCACGGGCTTATTCTTGAAGGTTTTGGCGTACT
GGCAGGGATTGATAACGATCCTTCTTGTAAGTATGCCTATGAGCAAAATAATAGAACTCGTTTTATTGAAAAGTCTATTA
CTGAAGTTGATGGCAGAGAGTTAAATGCCCTCTATCCCAATAATCAACATAAAATCTTAGTAGGCTGTGCCCCATGCCAA
GATTTTTCTCAATATACGAAGAAGAGTCGTACTGGAACAAAGTGGCAATTACTCACAGAATTTTCGCGCCTTATTAGAGA
GATCGAGCCTGATATTATATCAATGGAAAATGTTCCTGAGGTTCGAACATTCAATAGAGGGGAAGTTTTTAACAATTTTA
TTCAAGCACTTGAACAATTAGGGTATCACGTTTCGCATAGCGTGGTGCATTGTCCTGATTATGGAATTCCGCAGCAACGT
GACCGACTAGTCTTATTTGCTGCTAAACAGGGCGTTATTAAGATTATACCCCCAACCCACACTCCTGAGAACTATCGAAC
TGTACGTGAAGTTATTGGTTCTCTGCCACCAATTACTGCTGGCGGACACTGGGAGGGTGATAGCATGCATGCAGCCAGCA
GACTAGAAGATATAAACCTTCGACGTATTCAGCATTCTGTGCCTGGTGGTACTTGGGCCGATTGGCCGGAAGAATTGATT
GCAGAATGTCATAAAAAGGAAAGCGGTGAGAGTTATGGGAGCGTCTATGGTCGAATGGAATGGGATAAAGTAGCACCTAC
CATCACTACACAATGCAATGGGTATGGTAATGGTCGCTTTGGTCATCCAGAGCAGGATCGCGCTATTTCGCTCCGTGAGG
CTGCCTTACTTCAGACATTTCCGCGAAGTTATCAATTTGCCCCTGAAGGCCAACTGAAATTTAAGACAGTTAGTCGTCAA
ATAGGGAATGCTGTTCCGGTCGCACTAGGTCGTGTTATTGCAAAAAGTATTAAGCGTTTTTTGGAGGGTTTACATGAGCG
ACAGCGGGTACGAATTATCATTTAG

Upstream 100 bases:

>100_bases
TGAAGGAGGTAGTTAAAATCCCCCTCGATGAGAAGTACATTCTGTGATATGCTCTGTACTCGATAGTACCATTCACTCTT
GATGCTTTGTGGAGGTTAGC

Downstream 100 bases:

>100_bases
TCTTAATACATTGAATCACTTAAGCGAAGGTCTTTATAGTAATATTCCTGCGGTTTTATCTGAATTAGTTGCTAATGCTT
GGGATGCCGATGCAACTGAG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MVGAVIDLFCGVGGLTHGLILEGFGVLAGIDNDPSCKYAYEQNNRTRFIEKSITEVDGRELNALYPNNQHKILVGCAPCQ
DFSQYTKKSRTGTKWQLLTEFSRLIREIEPDIISMENVPEVRTFNRGEVFNNFIQALEQLGYHVSHSVVHCPDYGIPQQR
DRLVLFAAKQGVIKIIPPTHTPENYRTVREVIGSLPPITAGGHWEGDSMHAASRLEDINLRRIQHSVPGGTWADWPEELI
AECHKKESGESYGSVYGRMEWDKVAPTITTQCNGYGNGRFGHPEQDRAISLREAALLQTFPRSYQFAPEGQLKFKTVSRQ
IGNAVPVALGRVIAKSIKRFLEGLHERQRVRIII

Sequences:

>Translated_354_residues
MVGAVIDLFCGVGGLTHGLILEGFGVLAGIDNDPSCKYAYEQNNRTRFIEKSITEVDGRELNALYPNNQHKILVGCAPCQ
DFSQYTKKSRTGTKWQLLTEFSRLIREIEPDIISMENVPEVRTFNRGEVFNNFIQALEQLGYHVSHSVVHCPDYGIPQQR
DRLVLFAAKQGVIKIIPPTHTPENYRTVREVIGSLPPITAGGHWEGDSMHAASRLEDINLRRIQHSVPGGTWADWPEELI
AECHKKESGESYGSVYGRMEWDKVAPTITTQCNGYGNGRFGHPEQDRAISLREAALLQTFPRSYQFAPEGQLKFKTVSRQ
IGNAVPVALGRVIAKSIKRFLEGLHERQRVRIII
>Mature_354_residues
MVGAVIDLFCGVGGLTHGLILEGFGVLAGIDNDPSCKYAYEQNNRTRFIEKSITEVDGRELNALYPNNQHKILVGCAPCQ
DFSQYTKKSRTGTKWQLLTEFSRLIREIEPDIISMENVPEVRTFNRGEVFNNFIQALEQLGYHVSHSVVHCPDYGIPQQR
DRLVLFAAKQGVIKIIPPTHTPENYRTVREVIGSLPPITAGGHWEGDSMHAASRLEDINLRRIQHSVPGGTWADWPEELI
AECHKKESGESYGSVYGRMEWDKVAPTITTQCNGYGNGRFGHPEQDRAISLREAALLQTFPRSYQFAPEGQLKFKTVSRQ
IGNAVPVALGRVIAKSIKRFLEGLHERQRVRIII

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39809; Mature: 39809

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGAVIDLFCGVGGLTHGLILEGFGVLAGIDNDPSCKYAYEQNNRTRFIEKSITEVDGRE
CCHHHHHHHHCCCHHHHHHHHHCCHHEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCE
LNALYPNNQHKILVGCAPCQDFSQYTKKSRTGTKWQLLTEFSRLIREIEPDIISMENVPE
EEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
VRTFNRGEVFNNFIQALEQLGYHVSHSVVHCPDYGIPQQRDRLVLFAAKQGVIKIIPPTH
HHCCCCHHHHHHHHHHHHHHCHHHHCCEEECCCCCCCCCCCCEEEEEECCCEEEEECCCC
TPENYRTVREVIGSLPPITAGGHWEGDSMHAASRLEDINLRRIQHSVPGGTWADWPEELI
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AECHKKESGESYGSVYGRMEWDKVAPTITTQCNGYGNGRFGHPEQDRAISLREAALLQTF
HHHHHHCCCCHHHHHHCCCCHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PRSYQFAPEGQLKFKTVSRQIGNAVPVALGRVIAKSIKRFLEGLHERQRVRIII
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MVGAVIDLFCGVGGLTHGLILEGFGVLAGIDNDPSCKYAYEQNNRTRFIEKSITEVDGRE
CCHHHHHHHHCCCHHHHHHHHHCCHHEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCE
LNALYPNNQHKILVGCAPCQDFSQYTKKSRTGTKWQLLTEFSRLIREIEPDIISMENVPE
EEEECCCCCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
VRTFNRGEVFNNFIQALEQLGYHVSHSVVHCPDYGIPQQRDRLVLFAAKQGVIKIIPPTH
HHCCCCHHHHHHHHHHHHHHCHHHHCCEEECCCCCCCCCCCCEEEEEECCCEEEEECCCC
TPENYRTVREVIGSLPPITAGGHWEGDSMHAASRLEDINLRRIQHSVPGGTWADWPEELI
CCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
AECHKKESGESYGSVYGRMEWDKVAPTITTQCNGYGNGRFGHPEQDRAISLREAALLQTF
HHHHHHCCCCHHHHHHCCCCHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHC
PRSYQFAPEGQLKFKTVSRQIGNAVPVALGRVIAKSIKRFLEGLHERQRVRIII
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]