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The map label for this gene is thlA [H]

Identifier: 159897052

GI number: 159897052

Start: 607326

End: 608513

Strand: Direct

Name: thlA [H]

Synonym: Haur_0522

Alternate gene names: 159897052

Gene position: 607326-608513 (Clockwise)

Preceding gene: 159897051

Following gene: 159897056

Centisome position: 9.57

GC content: 54.38

Gene sequence:

>1188_bases
ATGAGCAACGAGCGCGAACCGGTGTTGTTGAGTGCTGCCCGCACGCCAATTGGCAAGTTTGGCGGGAGCCTCGCTGATTT
AACTGCGCCTCAACTCGGTGCGATCGCAATTAAGGCCGCATTGGAGCGAGCTGGAATCAACGGCGATGTGGTTGATGAAG
TGATTATGGGTAATGTCGTTGGGGCGGGCTTGGGTCAAGCACCTGCGCGACAAGCAGCTTTGGGTGCTGGTGTGCCCGAT
AGCGTGAGCGCCCTAACAATTAATAAGGTTTGTGGTTCGGGCTTAAAGGCCGTGATGTTGGCCGCCAGCATGATCAAAGC
TGGTGATGCTGAGCTGATTGTGGCTGGTGGCATGGAAAACATGTCGCGAGCGCCCTACCTTTTGCCTCAAGCCCGCTTTG
GCTATCGGCTTGGCAACGGTGAAATTCAAGATGCCGTAGTGCATGATGGCTTGTGGTGTGCGGTTGAGCATCATCATATG
GGCAACTCAGCTGAGTGGGTCGCCGATGCTTGCAATGTTAGTCGCGAAATGCAAGATGAATTTGCCTACAATTCACATCG
CAAAGCAGCAGCAGCGATTGCTCGTGGCGCGTTCAAAAACGAAATTGTGCCTGTTGAACTCAAGAGCCGCAAAGGCACAA
CCATTTTTGATACCGACGAGCCAGTTCGTGCCGATAGCACGGTTGAAGCCTTGGCCAAATTAAAGCCAGCCTTCAAAGCT
GATGGCTCTGTTACTGCTGGCAATGCACCTGGCATTACCGATGGCGCTTCGGCTTTGGTGGTCGCAAGCATGGCCAAAGC
CCAACAACTGGGAGCCAAGCCGCTGGCCCGCATTACTGGCTATGCCCAGGCTGGCGTGAAACCACTCGAAATCTTTACAG
CTCCAGCCTTTGCTGTGCGCCGCTTGCTTGAAGTAACCAACACCAGCTTGGCCGATTACGATTTGTATGAATTCAATGAA
GCCTTTGCCGCCCAAGCGCTTGCCAACGGCCACGACCTCAAGATCGATTGGGATCGCTTGAATGTCAATGGTGGAGCGGT
CGCTTTGGGTCACCCGATTGGGGCTAGCGGCGCACGCGTATTGACGACGCTGGTGTATGCCTTGCAAGAACGTGGTGGCA
AGCGTGGCCTCGCGACCCTCTGTTTAGGTGGTGGCGAAGCCGTTGCCTTGGCAATTGAATTGATTTAA

Upstream 100 bases:

>100_bases
TACACTTTATGGTAAGATAGTCAACAATTATTGAATAGATAAGCCAGTGGCAACGGCGCTGCTGGCTTTTAGCTAGTTCA
AAATCAAGGAGTATGGCCCC

Downstream 100 bases:

>100_bases
GCCAGAGCGGAGAAGGTGATGACGGCACCAAACAACCAACATAAGGAGGAACAATGGGTACTGTTGAGTTGATCTCTACT
TTGGATGAAGGCGTTCGTGA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSNEREPVLLSAARTPIGKFGGSLADLTAPQLGAIAIKAALERAGINGDVVDEVIMGNVVGAGLGQAPARQAALGAGVPD
SVSALTINKVCGSGLKAVMLAASMIKAGDAELIVAGGMENMSRAPYLLPQARFGYRLGNGEIQDAVVHDGLWCAVEHHHM
GNSAEWVADACNVSREMQDEFAYNSHRKAAAAIARGAFKNEIVPVELKSRKGTTIFDTDEPVRADSTVEALAKLKPAFKA
DGSVTAGNAPGITDGASALVVASMAKAQQLGAKPLARITGYAQAGVKPLEIFTAPAFAVRRLLEVTNTSLADYDLYEFNE
AFAAQALANGHDLKIDWDRLNVNGGAVALGHPIGASGARVLTTLVYALQERGGKRGLATLCLGGGEAVALAIELI

Sequences:

>Translated_395_residues
MSNEREPVLLSAARTPIGKFGGSLADLTAPQLGAIAIKAALERAGINGDVVDEVIMGNVVGAGLGQAPARQAALGAGVPD
SVSALTINKVCGSGLKAVMLAASMIKAGDAELIVAGGMENMSRAPYLLPQARFGYRLGNGEIQDAVVHDGLWCAVEHHHM
GNSAEWVADACNVSREMQDEFAYNSHRKAAAAIARGAFKNEIVPVELKSRKGTTIFDTDEPVRADSTVEALAKLKPAFKA
DGSVTAGNAPGITDGASALVVASMAKAQQLGAKPLARITGYAQAGVKPLEIFTAPAFAVRRLLEVTNTSLADYDLYEFNE
AFAAQALANGHDLKIDWDRLNVNGGAVALGHPIGASGARVLTTLVYALQERGGKRGLATLCLGGGEAVALAIELI
>Mature_394_residues
SNEREPVLLSAARTPIGKFGGSLADLTAPQLGAIAIKAALERAGINGDVVDEVIMGNVVGAGLGQAPARQAALGAGVPDS
VSALTINKVCGSGLKAVMLAASMIKAGDAELIVAGGMENMSRAPYLLPQARFGYRLGNGEIQDAVVHDGLWCAVEHHHMG
NSAEWVADACNVSREMQDEFAYNSHRKAAAAIARGAFKNEIVPVELKSRKGTTIFDTDEPVRADSTVEALAKLKPAFKAD
GSVTAGNAPGITDGASALVVASMAKAQQLGAKPLARITGYAQAGVKPLEIFTAPAFAVRRLLEVTNTSLADYDLYEFNEA
FAAQALANGHDLKIDWDRLNVNGGAVALGHPIGASGARVLTTLVYALQERGGKRGLATLCLGGGEAVALAIELI

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=388, Percent_Identity=45.8762886597938, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI4557237, Length=392, Percent_Identity=44.3877551020408, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI167614485, Length=394, Percent_Identity=41.3705583756345, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI4501853, Length=393, Percent_Identity=36.1323155216285, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=33.0985915492958, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=28.7531806615776, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1788554, Length=390, Percent_Identity=57.1794871794872, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=52.6992287917738, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=42.0792079207921, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=39.0428211586902, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788683, Length=408, Percent_Identity=29.656862745098, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=393, Percent_Identity=42.7480916030534, Blast_Score=310, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=44.3298969072165, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=40.6091370558376, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17535917, Length=399, Percent_Identity=35.3383458646617, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=35.1543942992874, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=45.088161209068, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6322031, Length=400, Percent_Identity=34.75, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24655093, Length=385, Percent_Identity=52.2077922077922, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=46.0154241645244, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI17648125, Length=393, Percent_Identity=42.2391857506361, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=32.1596244131455, Blast_Score=185, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40860; Mature: 40729

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEREPVLLSAARTPIGKFGGSLADLTAPQLGAIAIKAALERAGINGDVVDEVIMGNVV
CCCCCCCEEEEECCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GAGLGQAPARQAALGAGVPDSVSALTINKVCGSGLKAVMLAASMIKAGDAELIVAGGMEN
CCCCCCCCHHHHHHCCCCCCCCHHEEHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCC
MSRAPYLLPQARFGYRLGNGEIQDAVVHDGLWCAVEHHHMGNSAEWVADACNVSREMQDE
CCCCCEECCCHHHCEEECCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FAYNSHRKAAAAIARGAFKNEIVPVELKSRKGTTIFDTDEPVRADSTVEALAKLKPAFKA
HHHCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHCHHHCC
DGSVTAGNAPGITDGASALVVASMAKAQQLGAKPLARITGYAQAGVKPLEIFTAPAFAVR
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHH
RLLEVTNTSLADYDLYEFNEAFAAQALANGHDLKIDWDRLNVNGGAVALGHPIGASGARV
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEECCCCCCCHHHH
LTTLVYALQERGGKRGLATLCLGGGEAVALAIELI
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
SNEREPVLLSAARTPIGKFGGSLADLTAPQLGAIAIKAALERAGINGDVVDEVIMGNVV
CCCCCCEEEEECCCCHHHHCCCHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GAGLGQAPARQAALGAGVPDSVSALTINKVCGSGLKAVMLAASMIKAGDAELIVAGGMEN
CCCCCCCCHHHHHHCCCCCCCCHHEEHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCC
MSRAPYLLPQARFGYRLGNGEIQDAVVHDGLWCAVEHHHMGNSAEWVADACNVSREMQDE
CCCCCEECCCHHHCEEECCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
FAYNSHRKAAAAIARGAFKNEIVPVELKSRKGTTIFDTDEPVRADSTVEALAKLKPAFKA
HHHCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHCHHHCC
DGSVTAGNAPGITDGASALVVASMAKAQQLGAKPLARITGYAQAGVKPLEIFTAPAFAVR
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHH
RLLEVTNTSLADYDLYEFNEAFAAQALANGHDLKIDWDRLNVNGGAVALGHPIGASGARV
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEECCCCCCCHHHH
LTTLVYALQERGGKRGLATLCLGGGEAVALAIELI
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]