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The map label for this gene is metB [H]

Identifier: 159897038

GI number: 159897038

Start: 589697

End: 590830

Strand: Direct

Name: metB [H]

Synonym: Haur_0506

Alternate gene names: 159897038

Gene position: 589697-590830 (Clockwise)

Preceding gene: 159897037

Following gene: 159897047

Centisome position: 9.29

GC content: 50.97

Gene sequence:

>1134_bases
GTGGATTATGGCTTTGCAACCCGCGCGATTCATGCAGGGCAAGACCCTGAATCAGTAACTGGCGCTGTGATTGTGCCAAT
TTATCAAACTTCGACCTATGCCCAACGCGGAGTTGGCGATCATACTGGCTATGAATATTCACGTACCGATAATCCAACCC
GCACTGCCTTGCAAACGTGTTTAGCCGCTCTGGAAGAGGCCAAACATGCCTTGGTGTTTGCTTCGGGATTGGGGGCTTCA
ACCACCTTGATGCTTATGCTTAAGGCTGGCGATCATGTGATTTGTGGCGATGATGTTTATGGTGGCACCTATCGGCTGTT
CCAACGGGTGATGACTGAGCATGGCCTGAGCTTTGATTTTGTCGATATGGCTGATCCTGAGGCGGTTCGAGCGGCAATCA
AGCCTAATACTCGCTTGATTTGGCTGGAAACCCCAACCAACCCGCTATTAAAGCTCGCGCCGATTGCTGCTATTACCAAA
GTTGCCCGTGAACATGGCATTTGGACGATTGTTGATAACACCTTTGCTTCGCCCTACAACCAACGCCCAATTACCTTAGG
TGCGGATATGGTGCTGCACAGCACAACCAAATATATCGGCGGCCATAGCGATGTGGTTGGCGGGGCGATTATGACCTCGA
ACGATGAGCTGTATGAAAAATTGAAGTTCTTGCAAAATGCCGCTGGCGCTGTACCAGGCCCATTCGATTGCTGGTTGGTC
TTGCGCGGAGTCAAAACTCTGAGCATTCGCATGCGCGAGCACGAACGCAACGCTTTGGCAATTGCTCAATTCCTAACCGA
GCATCCAGCAGTTGAAAAAGTGATTTATCCAGGCTTGCCGTCGCATCCTCAGCACAACTTGGCGCGTGAGCAAATGCGCG
GCTTTGGCGGGATGATCTCAATCTTGCTGAAGGGCGGGGCGGAAGTTGCTAACGCCATGGTCAGCAAAACCAAGCTCTTT
ACCTTAGCTGAAAGCTTGGGCGGGATTGAATCGCTGATTGAAGTGCCAGCTGGCATGACCCATATGAGCGTTGCTGGCTC
GAAACTTGAAGTACCCGCCAATTTAGTGCGGCTTTCGGTTGGAATCGAAGATATTAATGATTTATTGCGCGACCTTGAGC
AAGCACTTGCGTAG

Upstream 100 bases:

>100_bases
GCCGTTTGTGGTTTTAGGCATTTTCTTCACTCTGTAAAATCAGCGTGCTATAATCGCATAGAGGCAATGTCGCCTTTTTG
CAATACGAAGGAGCACACTT

Downstream 100 bases:

>100_bases
TACTTATGGTTAAGCCGCTATAGCCATGGCTTGGATTAGGCTATGGCTGTCTCGCTCGCTAATCCAAGCTCGGTTCTTTC
TTGTTGGAGGTTGGCTATGA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDYGFATRAIHAGQDPESVTGAVIVPIYQTSTYAQRGVGDHTGYEYSRTDNPTRTALQTCLAALEEAKHALVFASGLGAS
TTLMLMLKAGDHVICGDDVYGGTYRLFQRVMTEHGLSFDFVDMADPEAVRAAIKPNTRLIWLETPTNPLLKLAPIAAITK
VAREHGIWTIVDNTFASPYNQRPITLGADMVLHSTTKYIGGHSDVVGGAIMTSNDELYEKLKFLQNAAGAVPGPFDCWLV
LRGVKTLSIRMREHERNALAIAQFLTEHPAVEKVIYPGLPSHPQHNLAREQMRGFGGMISILLKGGAEVANAMVSKTKLF
TLAESLGGIESLIEVPAGMTHMSVAGSKLEVPANLVRLSVGIEDINDLLRDLEQALA

Sequences:

>Translated_377_residues
MDYGFATRAIHAGQDPESVTGAVIVPIYQTSTYAQRGVGDHTGYEYSRTDNPTRTALQTCLAALEEAKHALVFASGLGAS
TTLMLMLKAGDHVICGDDVYGGTYRLFQRVMTEHGLSFDFVDMADPEAVRAAIKPNTRLIWLETPTNPLLKLAPIAAITK
VAREHGIWTIVDNTFASPYNQRPITLGADMVLHSTTKYIGGHSDVVGGAIMTSNDELYEKLKFLQNAAGAVPGPFDCWLV
LRGVKTLSIRMREHERNALAIAQFLTEHPAVEKVIYPGLPSHPQHNLAREQMRGFGGMISILLKGGAEVANAMVSKTKLF
TLAESLGGIESLIEVPAGMTHMSVAGSKLEVPANLVRLSVGIEDINDLLRDLEQALA
>Mature_377_residues
MDYGFATRAIHAGQDPESVTGAVIVPIYQTSTYAQRGVGDHTGYEYSRTDNPTRTALQTCLAALEEAKHALVFASGLGAS
TTLMLMLKAGDHVICGDDVYGGTYRLFQRVMTEHGLSFDFVDMADPEAVRAAIKPNTRLIWLETPTNPLLKLAPIAAITK
VAREHGIWTIVDNTFASPYNQRPITLGADMVLHSTTKYIGGHSDVVGGAIMTSNDELYEKLKFLQNAAGAVPGPFDCWLV
LRGVKTLSIRMREHERNALAIAQFLTEHPAVEKVIYPGLPSHPQHNLAREQMRGFGGMISILLKGGAEVANAMVSKTKLF
TLAESLGGIESLIEVPAGMTHMSVAGSKLEVPANLVRLSVGIEDINDLLRDLEQALA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=377, Percent_Identity=53.315649867374, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI299473759, Length=377, Percent_Identity=47.7453580901857, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI25453487, Length=376, Percent_Identity=47.6063829787234, Blast_Score=324, Evalue=9e-89,
Organism=Escherichia coli, GI1790375, Length=375, Percent_Identity=41.3333333333333, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1789383, Length=395, Percent_Identity=26.8354430379747, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538055, Length=379, Percent_Identity=54.6174142480211, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17560056, Length=379, Percent_Identity=51.1873350923483, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71980708, Length=375, Percent_Identity=40, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6319307, Length=384, Percent_Identity=48.6979166666667, Blast_Score=347, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6321254, Length=387, Percent_Identity=33.3333333333333, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=332, Percent_Identity=34.3373493975904, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=31.4942528735632, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6321904, Length=336, Percent_Identity=30.0595238095238, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=52.755905511811, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40808; Mature: 40808

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYGFATRAIHAGQDPESVTGAVIVPIYQTSTYAQRGVGDHTGYEYSRTDNPTRTALQTC
CCCCCHHHHEECCCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
LAALEEAKHALVFASGLGASTTLMLMLKAGDHVICGDDVYGGTYRLFQRVMTEHGLSFDF
HHHHHHHHHEEEEECCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEE
VDMADPEAVRAAIKPNTRLIWLETPTNPLLKLAPIAAITKVAREHGIWTIVDNTFASPYN
ECCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
QRPITLGADMVLHSTTKYIGGHSDVVGGAIMTSNDELYEKLKFLQNAAGAVPGPFDCWLV
CCCEEECCHHHHHHHHHHCCCCCHHCCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHH
LRGVKTLSIRMREHERNALAIAQFLTEHPAVEKVIYPGLPSHPQHNLAREQMRGFGGMIS
HHHHHHHEEHHHHHCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
ILLKGGAEVANAMVSKTKLFTLAESLGGIESLIEVPAGMTHMSVAGSKLEVPANLVRLSV
HHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEHHCCCCCCCCCHHHEEEEC
GIEDINDLLRDLEQALA
CHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDYGFATRAIHAGQDPESVTGAVIVPIYQTSTYAQRGVGDHTGYEYSRTDNPTRTALQTC
CCCCCHHHHEECCCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
LAALEEAKHALVFASGLGASTTLMLMLKAGDHVICGDDVYGGTYRLFQRVMTEHGLSFDF
HHHHHHHHHEEEEECCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCEEE
VDMADPEAVRAAIKPNTRLIWLETPTNPLLKLAPIAAITKVAREHGIWTIVDNTFASPYN
ECCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
QRPITLGADMVLHSTTKYIGGHSDVVGGAIMTSNDELYEKLKFLQNAAGAVPGPFDCWLV
CCCEEECCHHHHHHHHHHCCCCCHHCCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHH
LRGVKTLSIRMREHERNALAIAQFLTEHPAVEKVIYPGLPSHPQHNLAREQMRGFGGMIS
HHHHHHHEEHHHHHCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
ILLKGGAEVANAMVSKTKLFTLAESLGGIESLIEVPAGMTHMSVAGSKLEVPANLVRLSV
HHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEHHCCCCCCCCCHHHEEEEC
GIEDINDLLRDLEQALA
CHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2020544 [H]