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The map label for this gene is nusA [H]

Identifier: 159897005

GI number: 159897005

Start: 552446

End: 553780

Strand: Direct

Name: nusA [H]

Synonym: Haur_0473

Alternate gene names: 159897005

Gene position: 552446-553780 (Clockwise)

Preceding gene: 159897004

Following gene: 159897006

Centisome position: 8.7

GC content: 51.39

Gene sequence:

>1335_bases
ATGAAAAGTGATTTTTACGCAGCTATTTCACAGATTGCCGCTGAACGTGGCATTCCCCGCGAGTCGGTGCAGGATGTTGT
CGAACAAGCCTTAATTTCTGCCTATCGGCGCTATTTGGGCAGTAATCCACCACCAGTTGACGTTAAGATTGAATTAGAGC
CAAATACTGGGCGGATTCGGGTTTACGCTGAAAAGCAAGTCGTCGATGAAGTGATGGATGATCGCTTCGAAATCGATATT
GAAGATGCCCGTAACGTTCGCGCCGATGTTGAAATTGGCGAAACGGTTTATGTGGAAAGCACGCCCGACGATTTTGGGCG
GATTGCCGCCCAAACCGCCAAACAGGTGGTATTGCAACGGATCAAAGAAGTTGAACGCGACCATATCTATGGCGAATACT
TTGATCGCGAAGGCGAAATTGTCACTGCCACCGTGCAGCGCACCGCCAAAGGCAACGTAATTTTAGAAGTTGGGCGAGCC
GAAGCGATTTTGCCCCAAAAAGAGCAAATTAGCCACGACAACTATCGCCATGGCCAACGCCTCAAAGTCTATTTGATGGA
AGCTCGCCGTGATGATCCGCGTGGCCCGCGCTTGGTCGCCTCGCGCACCCACAAAGATTTGATCAAACGCTTATTTGAAA
TGGAAGTGCCCGAAATCTACAACGGCACGGTTGAAATTAAATCGATCGCCCGTGAACCAGGTTTACGCTCGAAAGTCGCC
GTCCATGCCCGTCAAGAAGGCATCGATCCGGTTGGCTCGTGCGTGGGGATGCGCGGGATTCGGATTCAAAATATTGTGAA
TGAACTGAACGGCGAGAAAATCGACGTGGTGCAATGGGGTGCTGATATGCGGGTATTTATTGCCAACGCCCTCAGCCCAG
CCCAAGTCGTCGAAGTTCATCTTGATGAAGGCGAAAAAACGGCCACGGTGGTCGTGCCAGATAAACAATTGTCGTTGGCA
ATTGGCAAGGAGGGCCAAAACGTTCGTTTGGCAGCCAAACTGGTTGGCTGGCGCATCGACATCAAGAGCGCATCTTCACT
CTTAGAGGAAGAACGGGCTGCTGCTGAAGCGCGTGAGGCTGCCGCGTCGGAACAAATGCTGCAAGAAGCAGCGCTCTCAA
CCGCCAAAGTTGAAACCCGCAAGGTGCGGGTCGATTCCTTGGTCACCTATCAAGGGCGACAATATGGCCCCTTGCCAGTT
GAACTAATTGGCGAAGAAGTAGCGTTGCGAGCCGCCGCCCAAAAACTCAATATTTATTTCAATGACAAGCTGATTGCTAG
CTATATCATCGATGATGAGGCTGGTGACAGCGACGAGACGGATACCGAGGCATAG

Upstream 100 bases:

>100_bases
GTTTCTTCTTGTCAGCAATGTTAACCAACGCAGGGTGGCTCGATCAAAGCAGGTATTATGCTGATGCCTGACCCACTGCA
CCCCCTAAGCGAGGCTAGAT

Downstream 100 bases:

>100_bases
CCTATGGCTGCTCAAAAGCAGTCGCCGCGCCCACGCCATGTGCCACAGCGCATGTGTGTTGCTTGTCGGCGTACCGAGAG
TAAACGTCAGTTGGTACGTC

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKSDFYAAISQIAAERGIPRESVQDVVEQALISAYRRYLGSNPPPVDVKIELEPNTGRIRVYAEKQVVDEVMDDRFEIDI
EDARNVRADVEIGETVYVESTPDDFGRIAAQTAKQVVLQRIKEVERDHIYGEYFDREGEIVTATVQRTAKGNVILEVGRA
EAILPQKEQISHDNYRHGQRLKVYLMEARRDDPRGPRLVASRTHKDLIKRLFEMEVPEIYNGTVEIKSIAREPGLRSKVA
VHARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWGADMRVFIANALSPAQVVEVHLDEGEKTATVVVPDKQLSLA
IGKEGQNVRLAAKLVGWRIDIKSASSLLEEERAAAEAREAAASEQMLQEAALSTAKVETRKVRVDSLVTYQGRQYGPLPV
ELIGEEVALRAAAQKLNIYFNDKLIASYIIDDEAGDSDETDTEA

Sequences:

>Translated_444_residues
MKSDFYAAISQIAAERGIPRESVQDVVEQALISAYRRYLGSNPPPVDVKIELEPNTGRIRVYAEKQVVDEVMDDRFEIDI
EDARNVRADVEIGETVYVESTPDDFGRIAAQTAKQVVLQRIKEVERDHIYGEYFDREGEIVTATVQRTAKGNVILEVGRA
EAILPQKEQISHDNYRHGQRLKVYLMEARRDDPRGPRLVASRTHKDLIKRLFEMEVPEIYNGTVEIKSIAREPGLRSKVA
VHARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWGADMRVFIANALSPAQVVEVHLDEGEKTATVVVPDKQLSLA
IGKEGQNVRLAAKLVGWRIDIKSASSLLEEERAAAEAREAAASEQMLQEAALSTAKVETRKVRVDSLVTYQGRQYGPLPV
ELIGEEVALRAAAQKLNIYFNDKLIASYIIDDEAGDSDETDTEA
>Mature_444_residues
MKSDFYAAISQIAAERGIPRESVQDVVEQALISAYRRYLGSNPPPVDVKIELEPNTGRIRVYAEKQVVDEVMDDRFEIDI
EDARNVRADVEIGETVYVESTPDDFGRIAAQTAKQVVLQRIKEVERDHIYGEYFDREGEIVTATVQRTAKGNVILEVGRA
EAILPQKEQISHDNYRHGQRLKVYLMEARRDDPRGPRLVASRTHKDLIKRLFEMEVPEIYNGTVEIKSIAREPGLRSKVA
VHARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWGADMRVFIANALSPAQVVEVHLDEGEKTATVVVPDKQLSLA
IGKEGQNVRLAAKLVGWRIDIKSASSLLEEERAAAEAREAAASEQMLQEAALSTAKVETRKVRVDSLVTYQGRQYGPLPV
ELIGEEVALRAAAQKLNIYFNDKLIASYIIDDEAGDSDETDTEA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=358, Percent_Identity=35.4748603351955, Blast_Score=237, Evalue=1e-63,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 49543; Mature: 49543

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDFYAAISQIAAERGIPRESVQDVVEQALISAYRRYLGSNPPPVDVKIELEPNTGRIR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEE
VYAEKQVVDEVMDDRFEIDIEDARNVRADVEIGETVYVESTPDDFGRIAAQTAKQVVLQR
EEEHHHHHHHHHCCCEEEEEHHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHH
IKEVERDHIYGEYFDREGEIVTATVQRTAKGNVILEVGRAEAILPQKEQISHDNYRHGQR
HHHHHHHCCCHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCE
LKVYLMEARRDDPRGPRLVASRTHKDLIKRLFEMEVPEIYNGTVEIKSIAREPGLRSKVA
EEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHCCEEEEHHHHCCCCCCHHHH
VHARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWGADMRVFIANALSPAQVVEVH
HHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCEEEEEE
LDEGEKTATVVVPDKQLSLAIGKEGQNVRLAAKLVGWRIDIKSASSLLEEERAAAEAREA
ECCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHH
AASEQMLQEAALSTAKVETRKVRVDSLVTYQGRQYGPLPVELIGEEVALRAAAQKLNIYF
HHHHHHHHHHHHHHHHHHHHEEEEHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEE
NDKLIASYIIDDEAGDSDETDTEA
CCEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKSDFYAAISQIAAERGIPRESVQDVVEQALISAYRRYLGSNPPPVDVKIELEPNTGRIR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEE
VYAEKQVVDEVMDDRFEIDIEDARNVRADVEIGETVYVESTPDDFGRIAAQTAKQVVLQR
EEEHHHHHHHHHCCCEEEEEHHCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHH
IKEVERDHIYGEYFDREGEIVTATVQRTAKGNVILEVGRAEAILPQKEQISHDNYRHGQR
HHHHHHHCCCHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCHHHCCCCCCCCCCE
LKVYLMEARRDDPRGPRLVASRTHKDLIKRLFEMEVPEIYNGTVEIKSIAREPGLRSKVA
EEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHCCEEEEHHHHCCCCCCHHHH
VHARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWGADMRVFIANALSPAQVVEVH
HHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCEEEEEE
LDEGEKTATVVVPDKQLSLAIGKEGQNVRLAAKLVGWRIDIKSASSLLEEERAAAEAREA
ECCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHH
AASEQMLQEAALSTAKVETRKVRVDSLVTYQGRQYGPLPVELIGEEVALRAAAQKLNIYF
HHHHHHHHHHHHHHHHHHHHEEEEHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEE
NDKLIASYIIDDEAGDSDETDTEA
CCEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]