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The map label for this gene is sphR [H]

Identifier: 159896979

GI number: 159896979

Start: 524641

End: 525330

Strand: Direct

Name: sphR [H]

Synonym: Haur_0447

Alternate gene names: 159896979

Gene position: 524641-525330 (Clockwise)

Preceding gene: 159896978

Following gene: 159896980

Centisome position: 8.27

GC content: 51.01

Gene sequence:

>690_bases
ATGAGTCGCATCCTGATTGTCGATGATGATCCAGCGATTTTGAGTGGGGTCAGCACCTTGTTGGAGCAAGCAGGCTATGA
GATTGCTCAGGCGGCTAGTTTGGCTCAAGCTCGTTCGCATTTGCAGGCATTTAATCCCCCAAGCTTGGTGATTCTCGATT
TGATGCTGCCTGATGGTGATGGCTTTGAGTTGTGTCGCCAAATTCGCCAAGGTGGCCAATATCTGCCAATTCTGATGCTT
TCAGCCCGCGATGAATTACAGGATCGGGTTGAAGGCTTGACGGTTGGCGCTGATGATTATCTGACCAAGCCGTTTGCACC
TGCCGAATTAGTGGCTCGGGTGCGGGCGATTTTGCGCTTGGCGCAACACCAACGTGAGACGATTGGCTCATTCGAATGCG
GCAAATTGCGCTTCGATTGCGAATTACAACGCGCTTGGTGGGATGAGCAATTACTCGAATTAACGCCTAAAGAAAGCGGA
GTTTTGTTACAGCTGATGCGTCAGCCGAGCCATGTTTGGGGGCGTTTGGCCTTGCTCCAAACCGTTTGGGGCACAAATTT
CTTGGGCGATTCGCGGATTGTTGATGTGTGTGTGCAACGCTTACGTGCTAAAATCAACCAACTTGATGCGAATGCCGACC
CGATTCAGACCGTGCGTGGTTTTGGCTATCGGTTAAAGTGTGAGCCATGA

Upstream 100 bases:

>100_bases
TAACAATCAGGTAACAATTGGCTGATAAACGCCTGATGTTGCTCTGAGACTTGTTTTTTTGGCTAAAATAGCGCTATGCA
ACAGTTTGGAGGTTTGGGCT

Downstream 100 bases:

>100_bases
TTGTTCTACCGCTCAAATCGTTGCTTTTTGGAATTTTATTGCTACTGTGGCTGGTTGCCAGCTTTGCTTGGTGGCTTGGT
CAGCGTGGCAAACGCCCCAT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSRILIVDDDPAILSGVSTLLEQAGYEIAQAASLAQARSHLQAFNPPSLVILDLMLPDGDGFELCRQIRQGGQYLPILML
SARDELQDRVEGLTVGADDYLTKPFAPAELVARVRAILRLAQHQRETIGSFECGKLRFDCELQRAWWDEQLLELTPKESG
VLLQLMRQPSHVWGRLALLQTVWGTNFLGDSRIVDVCVQRLRAKINQLDANADPIQTVRGFGYRLKCEP

Sequences:

>Translated_229_residues
MSRILIVDDDPAILSGVSTLLEQAGYEIAQAASLAQARSHLQAFNPPSLVILDLMLPDGDGFELCRQIRQGGQYLPILML
SARDELQDRVEGLTVGADDYLTKPFAPAELVARVRAILRLAQHQRETIGSFECGKLRFDCELQRAWWDEQLLELTPKESG
VLLQLMRQPSHVWGRLALLQTVWGTNFLGDSRIVDVCVQRLRAKINQLDANADPIQTVRGFGYRLKCEP
>Mature_228_residues
SRILIVDDDPAILSGVSTLLEQAGYEIAQAASLAQARSHLQAFNPPSLVILDLMLPDGDGFELCRQIRQGGQYLPILMLS
ARDELQDRVEGLTVGADDYLTKPFAPAELVARVRAILRLAQHQRETIGSFECGKLRFDCELQRAWWDEQLLELTPKESGV
LLQLMRQPSHVWGRLALLQTVWGTNFLGDSRIVDVCVQRLRAKINQLDANADPIQTVRGFGYRLKCEP

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=38.8646288209607, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=38.2882882882883, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1790552, Length=223, Percent_Identity=38.5650224215247, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=37.6106194690265, Blast_Score=133, Evalue=8e-33,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=35.2422907488987, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789809, Length=230, Percent_Identity=34.7826086956522, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87082012, Length=226, Percent_Identity=37.6106194690265, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=37.219730941704, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=36.8421052631579, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=33.6322869955157, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.9912663755458, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=28.82096069869, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787229, Length=234, Percent_Identity=32.4786324786325, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=29.957805907173, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=32.5396825396825, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25665; Mature: 25534

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRILIVDDDPAILSGVSTLLEQAGYEIAQAASLAQARSHLQAFNPPSLVILDLMLPDGD
CCEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GFELCRQIRQGGQYLPILMLSARDELQDRVEGLTVGADDYLTKPFAPAELVARVRAILRL
CHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCEEECCHHHHCCCCCHHHHHHHHHHHHHH
AQHQRETIGSFECGKLRFDCELQRAWWDEQLLELTPKESGVLLQLMRQPSHVWGRLALLQ
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHH
TVWGTNFLGDSRIVDVCVQRLRAKINQLDANADPIQTVRGFGYRLKCEP
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SRILIVDDDPAILSGVSTLLEQAGYEIAQAASLAQARSHLQAFNPPSLVILDLMLPDGD
CEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GFELCRQIRQGGQYLPILMLSARDELQDRVEGLTVGADDYLTKPFAPAELVARVRAILRL
CHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCEEECCHHHHCCCCCHHHHHHHHHHHHHH
AQHQRETIGSFECGKLRFDCELQRAWWDEQLLELTPKESGVLLQLMRQPSHVWGRLALLQ
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHH
TVWGTNFLGDSRIVDVCVQRLRAKINQLDANADPIQTVRGFGYRLKCEP
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]