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The map label for this gene is sigA [H]

Identifier: 159896899

GI number: 159896899

Start: 439342

End: 440517

Strand: Direct

Name: sigA [H]

Synonym: Haur_0367

Alternate gene names: 159896899

Gene position: 439342-440517 (Clockwise)

Preceding gene: 159896894

Following gene: 159896910

Centisome position: 6.92

GC content: 52.13

Gene sequence:

>1176_bases
ATGTTTACCAGACGTAACGTAGTAGATTGCATAGAGAATCCTGCGGGTTCAATAGATAGCTTTCGTGCGCCGCCAGCAGC
GAAAGCCATAGTGGGCGTAGCCCAGCGAGCGTGGACGATGACAAAGTATGAAACGATGAATGTAGCAAAAAACCAACCGG
AACTCGAAGATGCCCCCGAGGGCTGGGAGCTTGAGCCGGGTTTTAATGATGAAGACGTAGAATTAGCAATTGTAAACCTA
CGCCGCAAAAAAACGCTCTATTCCGAGGCTGTCGAAGATTCAGTCCAGATGTATTTGCGTGAAATCGGCGAGGTTGAGTT
GCTCACCGCCGAGGAAGAAATTACGCTGGCCAAGCAAATTGAAACTGGCCGCAAAGCGGAACATCAGTTGCAAACCGAGC
AGTATGCAACCTGGGATGAACGTACCGCCCTCGAACGCCGCGTCGATTTTGGTAATGAAGGCCGTCGCAAGCTGACCCAA
GCCAATTTGCGCTTGGTCGTGAGCGTCGCCAAAAAATATATTGGCAATCACATGTCGTTCATGGATTTGATCCAAGAAGG
CAATATTGGTTTGATGCGGGCAGTTGAGAAGTTCGATTATCGCAAGGGTAATCGCTTTTCAACCTATGCTACGTGGTGGA
TTCGCCAAGCAGTTACCCGTGCGATCGCTGAGCAAAGCCGCACCATCCGCTTGCCAGTCCACTTGAGCGAATCAATCGCC
CAAATGCGCCGCGTCGCTTACCAGCTTGAGCAAGCTTTGCAACGTGAAGCAACGCCCGATGAATTGGCTGATGCCTTGGG
TGTAAGTTTGCGCAAGGTCAAGCGTATGCTGAATGCTTCGGTACAGCCAGTGTCGCTTGAGCAACCAATTGGCAAAGAAG
GCCAAGGCCGCGTTGGCGAATTTTTGGCCGACGATACCGATGAAGCACCGCTTGAGCAGGCCACCCGCATGATGTTGCAA
GATGAATTGGCCGATGCGCTCTCGCAATTGCCCGACCGCGAACGCCAAATCTTGTTGTTGCGCTATGGTTTGGCCGATGG
CAAACGCCGCACGCTCGAAGAAGTTGGCGCTGAATTCGGGATTACTCGCGAACGCACCCGCCAAATCGAGGCTGAAGCGA
TGCGTCACTTGCGCCAACCCAATGTTGGTCAACACTTGCGAGCTTACCTTGATTAA

Upstream 100 bases:

>100_bases
ACTTGTTATACTGTAACTGTAAGAATTACAGTTCGTGGCGTGGTTTTTGCTTAGCACAATCCAACTGGTAAGCAGCCCGT
CGCAATCCACCTGCACATCC

Downstream 100 bases:

>100_bases
TTGCTAAGCTCTGTTGGTTCAATCGCCCTCGCTGCCGTTTTGGTGGCGAGGGCTTTTTGATCCACGAAGGCCACAAAGGA
CACGAAGGGGAAATGTCTAT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFTRRNVVDCIENPAGSIDSFRAPPAAKAIVGVAQRAWTMTKYETMNVAKNQPELEDAPEGWELEPGFNDEDVELAIVNL
RRKKTLYSEAVEDSVQMYLREIGEVELLTAEEEITLAKQIETGRKAEHQLQTEQYATWDERTALERRVDFGNEGRRKLTQ
ANLRLVVSVAKKYIGNHMSFMDLIQEGNIGLMRAVEKFDYRKGNRFSTYATWWIRQAVTRAIAEQSRTIRLPVHLSESIA
QMRRVAYQLEQALQREATPDELADALGVSLRKVKRMLNASVQPVSLEQPIGKEGQGRVGEFLADDTDEAPLEQATRMMLQ
DELADALSQLPDRERQILLLRYGLADGKRRTLEEVGAEFGITRERTRQIEAEAMRHLRQPNVGQHLRAYLD

Sequences:

>Translated_391_residues
MFTRRNVVDCIENPAGSIDSFRAPPAAKAIVGVAQRAWTMTKYETMNVAKNQPELEDAPEGWELEPGFNDEDVELAIVNL
RRKKTLYSEAVEDSVQMYLREIGEVELLTAEEEITLAKQIETGRKAEHQLQTEQYATWDERTALERRVDFGNEGRRKLTQ
ANLRLVVSVAKKYIGNHMSFMDLIQEGNIGLMRAVEKFDYRKGNRFSTYATWWIRQAVTRAIAEQSRTIRLPVHLSESIA
QMRRVAYQLEQALQREATPDELADALGVSLRKVKRMLNASVQPVSLEQPIGKEGQGRVGEFLADDTDEAPLEQATRMMLQ
DELADALSQLPDRERQILLLRYGLADGKRRTLEEVGAEFGITRERTRQIEAEAMRHLRQPNVGQHLRAYLD
>Mature_391_residues
MFTRRNVVDCIENPAGSIDSFRAPPAAKAIVGVAQRAWTMTKYETMNVAKNQPELEDAPEGWELEPGFNDEDVELAIVNL
RRKKTLYSEAVEDSVQMYLREIGEVELLTAEEEITLAKQIETGRKAEHQLQTEQYATWDERTALERRVDFGNEGRRKLTQ
ANLRLVVSVAKKYIGNHMSFMDLIQEGNIGLMRAVEKFDYRKGNRFSTYATWWIRQAVTRAIAEQSRTIRLPVHLSESIA
QMRRVAYQLEQALQREATPDELADALGVSLRKVKRMLNASVQPVSLEQPIGKEGQGRVGEFLADDTDEAPLEQATRMMLQ
DELADALSQLPDRERQILLLRYGLADGKRRTLEEVGAEFGITRERTRQIEAEAMRHLRQPNVGQHLRAYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=268, Percent_Identity=45.8955223880597, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1789098, Length=292, Percent_Identity=38.013698630137, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789871, Length=262, Percent_Identity=28.6259541984733, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788231, Length=198, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 44684; Mature: 44684

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTRRNVVDCIENPAGSIDSFRAPPAAKAIVGVAQRAWTMTKYETMNVAKNQPELEDAPE
CCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GWELEPGFNDEDVELAIVNLRRKKTLYSEAVEDSVQMYLREIGEVELLTAEEEITLAKQI
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHH
ETGRKAEHQLQTEQYATWDERTALERRVDFGNEGRRKLTQANLRLVVSVAKKYIGNHMSF
HHCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MDLIQEGNIGLMRAVEKFDYRKGNRFSTYATWWIRQAVTRAIAEQSRTIRLPVHLSESIA
HHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHH
QMRRVAYQLEQALQREATPDELADALGVSLRKVKRMLNASVQPVSLEQPIGKEGQGRVGE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
FLADDTDEAPLEQATRMMLQDELADALSQLPDRERQILLLRYGLADGKRRTLEEVGAEFG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCHHHHHHHHHHHCC
ITRERTRQIEAEAMRHLRQPNVGQHLRAYLD
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MFTRRNVVDCIENPAGSIDSFRAPPAAKAIVGVAQRAWTMTKYETMNVAKNQPELEDAPE
CCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GWELEPGFNDEDVELAIVNLRRKKTLYSEAVEDSVQMYLREIGEVELLTAEEEITLAKQI
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHHH
ETGRKAEHQLQTEQYATWDERTALERRVDFGNEGRRKLTQANLRLVVSVAKKYIGNHMSF
HHCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
MDLIQEGNIGLMRAVEKFDYRKGNRFSTYATWWIRQAVTRAIAEQSRTIRLPVHLSESIA
HHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHH
QMRRVAYQLEQALQREATPDELADALGVSLRKVKRMLNASVQPVSLEQPIGKEGQGRVGE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
FLADDTDEAPLEQATRMMLQDELADALSQLPDRERQILLLRYGLADGKRRTLEEVGAEFG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCHHHHHHHHHHHCC
ITRERTRQIEAEAMRHLRQPNVGQHLRAYLD
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987246; 3086839; 8969508; 9384377; 2166045; 8363585 [H]