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The map label for this gene is splB

Identifier: 15927386

GI number: 15927386

Start: 1863563

End: 1864285

Strand: Reverse

Name: splB

Synonym: SA1630

Alternate gene names: 15927386

Gene position: 1864285-1863563 (Counterclockwise)

Preceding gene: 15927387

Following gene: 15927385

Centisome position: 66.23

GC content: 33.2

Gene sequence:

>723_bases
ATGAACAAAAACGTAGTCATCAAGAGTTTAGCAACTTTAACAATTTTAACATCTGTAACAGGTATTGGAACAACATTGGT
TGAGGAAGTTCAACAAACTGCCAAAGCAGAAAATAATGTCACAAAAATTCAAGATACTAATATTTTTCCATATACTGGTG
TAGTTGCTTTTAAAAGTGCAACTGGATTTGTAGTTGGAAAGAATACTATTTTAACAAATAAACATGTGTCGAAAAATTAC
AAAGTGGGCGATCGTATTACTGCACATCCAAATAGTGATAAAGGTAATGGTGGTATTTATTCGATTAAAAAGATTATTAA
TTATCCAGGTAAAGAAGATGTATCAGTCATTCAAGTTGAAGAGCGTGCAATAGAACGTGGACCAAAAGGCTTTAATTTTA
ATGATAATGTAACGCCATTCAAATATGCGGCAGGGGCTAAAGCTGGTGAGCGAATTAAAGTGATTGGTTATCCACACCCA
TACAAAAATAAATATGTTTTATATGAGTCAACTGGCCCTGTGATGTCAGTAGAAGGTAGCAGTATTGTATATTCAGCGCA
TACTGAAAGCGGAAACTCTGGATCACCTGTATTAAACAGCAACAACGAATTAGTTGGTATTCATTTTGCTTCTGATGTAA
AAAATGATGATAACAGAAATGCATATGGCGTCTACTTTACACCAGAAATTAAAAAATTCATTGCAGAAAACATAGATAAA
TAA

Upstream 100 bases:

>100_bases
TGATTTTTTAAAGAGTGATTAAGAAAACGGTTACATAACCAACTAAATATACTGAATTATGTATCTAGATATCAAAATAA
TTAAAAAAGAGGAACTTAAA

Downstream 100 bases:

>100_bases
ACAAATTGACTTTAAACGAGCGTTGCAACATATCTCGAATTGTAAAGGAGCTTGAAAATGAATAAAAATATAGTCATTAA
AAGCATGGCAGCATTAGCCA

Product: serine protease SplB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQDTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNY
KVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK

Sequences:

>Translated_240_residues
MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQDTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNY
KVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK
>Mature_240_residues
MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQDTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNY
KVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK

Specific function: Serine protease that cleaves specifically after the sequence Trp-Glu-Leu-Gln

COG id: COG3591

COG function: function code E; V8-like Glu-specific endopeptidase

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S1B family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPLB_STAA1 (A7X3Q8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442387.1
- ProteinModelPortal:   A7X3Q8
- SMR:   A7X3Q8
- STRING:   A7X3Q8
- MEROPS:   S01.282
- EnsemblBacteria:   EBSTAT00000002858
- GeneID:   5561235
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1797
- eggNOG:   COG3591
- GeneTree:   EBGT00050000023734
- HOGENOM:   HBG693141
- OMA:   EEHAIER
- ProtClustDB:   CLSK885576
- BioCyc:   SAUR418127:SAHV_1797-MONOMER
- GO:   GO:0006508
- InterPro:   IPR009003
- InterPro:   IPR000126
- InterPro:   IPR001254
- InterPro:   IPR008256
- InterPro:   IPR008353
- PRINTS:   PR01774
- PRINTS:   PR00839
- SMART:   SM00020

Pfam domain/function: PF00089 Trypsin; SSF50494 Pept_Ser_Cys

EC number: NA

Molecular weight: Translated: 26141; Mature: 26141

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00672 V8_HIS; PS00673 V8_SER

Important sites: ACT_SITE 75-75 ACT_SITE 113-113 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQDTNIFPYTGVVAFKSA
CCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECC
TGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVE
CCEEEECCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEH
ERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHPYKNKYVLYESTGPVMSVEGS
HHHHHCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEECCC
SIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK
EEEEEEECCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHCCC
>Mature Secondary Structure
MNKNVVIKSLATLTILTSVTGIGTTLVEEVQQTAKAENNVTKIQDTNIFPYTGVVAFKSA
CCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECC
TGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSIKKIINYPGKEDVSVIQVE
CCEEEECCEEEECCCCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEH
ERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHPYKNKYVLYESTGPVMSVEGS
HHHHHCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEECCC
SIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDNRNAYGVYFTPEIKKFIAENIDK
EEEEEEECCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA