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The map label for this gene is thyA

Identifier: 15927008

GI number: 15927008

Start: 1432585

End: 1433541

Strand: Reverse

Name: thyA

Synonym: SA1260

Alternate gene names: 15927008

Gene position: 1433541-1432585 (Counterclockwise)

Preceding gene: 15927009

Following gene: 15927007

Centisome position: 50.93

GC content: 33.65

Gene sequence:

>957_bases
ATGTTGAATTCATTTGATGCAGCATATCACAGTCTTTGTGAAGAGGTTTTAGAAATAGGAAATACACGAAATGATCGCAC
AAATACAGGTACGATTTCGAAATTTGGTCATCAACTTCGCTTTGACTTATCTAAAGGATTTCCACTATTAACGACAAAGA
AAGTTTCTTTTAAATTAGTAGCAACCGAATTATTATGGTTCATTAAAGGAGATACAAACATCCAATACTTATTAAAATAT
AATAATAATATTTGGAACGAATGGGCTTTTGAAAATTATATAAAATCAGACGAGTATAACGGTCCAGATATGACAGATTT
CGGGCATCGTGCATTGAGTGATCCTGAATTTAACGAACAATATAAAGAACAAATGAAACAATTTAAGCAACGTATTCTTG
AAGATGATACTTTTGCGAAGCAATTCGGTGATTTAGGAAATGTTTATGGTAAACAATGGCGAGATTGGGTTGATAAAGAT
GGTAATCATTTTGATCAACTTAAAACAGTAATTGAACAAATTAAGCATAATCCAGATTCAAGACGACACATCGTATCTGC
ATGGAATCCAACAGAAATTGATACAATGGCACTTCCGCCTTGTCATACCATGTTCCAGTTTTATGTCCAAGATGGTAAGT
TAAGTTGCCAGTTATACCAACGTAGCGCAGATATCTTTTTAGGTGTGCCATTTAATATCGCAAGCTACGCTTTATTGACA
CACCTTATTGCCAAAGAATGTGGACTTGAAGTAGGAGAATTTGTGCATACATTTGGAGATGCACATATTTATTCAAATCA
TATTGATGCGATTCAAACACAATTAGCACGTGAAAGCTTCAATCCTCCAACATTAAAAATTAACAGTGACAAGTCTATTT
TCGACATAAATTATGAAGATTTGGAAATTGTTGACTATGAATCACATCCAGCAATAAAAGCTCCAATAGCAGTGTAG

Upstream 100 bases:

>100_bases
CATATTCCTAAAATGAGTGATAACAAAATGCTACATAAAATTAAGTTATATCAAACTAAATATACATACTATAAATAATG
AAAATGAGGTGTTATCGCAT

Downstream 100 bases:

>100_bases
TCATTGCATAGTTAGCTAACCATATAGACATCAAAATGACATCATAGTATTTTCAAGTGCAAAAAAGTACTTTTTTGTGT
TAAACGTTTTCATAAATTAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKY
NNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV

Sequences:

>Translated_318_residues
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKY
NNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV
>Mature_318_residues
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKY
NNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=319, Percent_Identity=43.5736677115987, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1789191, Length=311, Percent_Identity=50.1607717041801, Blast_Score=307, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI71993377, Length=327, Percent_Identity=46.177370030581, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=197, Percent_Identity=51.7766497461929, Blast_Score=190, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17137556, Length=328, Percent_Identity=43.9024390243902, Blast_Score=260, Evalue=9e-70,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_STAA1 (A7X2B3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442005.1
- ProteinModelPortal:   A7X2B3
- SMR:   A7X2B3
- STRING:   A7X2B3
- EnsemblBacteria:   EBSTAT00000004173
- GeneID:   5561632
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1415
- eggNOG:   COG0207
- GeneTree:   EBGT00050000025191
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   SAUR418127:SAHV_1415-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 36826; Mature: 36826

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECCHHHHHH
ATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQ
HHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHH
YKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
RRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHH
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYED
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCC
LEIVDYESHPAIKAPIAV
EEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLSKGFPLLTTKKVSFKLV
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECCHHHHHH
ATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYNGPDMTDFGHRALSDPEFNEQ
HHHHHHHEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHH
YKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
RRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQLYQRSADIFLGVPFNIASYALLT
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCEEEECCHHHHHHHHHH
HLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYED
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCC
LEIVDYESHPAIKAPIAV
EEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA