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The map label for this gene is miaB

Identifier: 15926875

GI number: 15926875

Start: 1288297

End: 1289841

Strand: Direct

Name: miaB

Synonym: SA1134

Alternate gene names: 15926875

Gene position: 1288297-1289841 (Clockwise)

Preceding gene: 15926874

Following gene: 15926876

Centisome position: 45.77

GC content: 32.82

Gene sequence:

>1545_bases
GTGAACGAAGAACAAAGAAAAGCAAGTTCTGTAGATGTTTTAGCTGAGAGAGATAAGAAAGCAGAAAAAGATTATAGTAA
ATATTTTGAACATGTTTATCAGCCGCCTAATTTAAAAGAAGCGAAAAAAAGAGGTAAACAAGAAGTTCGTTATAATAGAG
ATTTCCAAATTGATGAAAAATATCGCGGTATGGGGAACGAGCGTACATTTTTAATTAAAACATATGGATGTCAAATGAAT
GCACATGACACTGAGGTCATTGCTGGTATACTTGAAGCATTAGGCTATCAAGCAACGACTGATATTAACACTGCAGATGT
TATTTTAATTAATACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTTTAGTGAAATAGGTAATTTGAAGCATTTGA
AAAAAGAACGACCTGATATTTTAATCGGTGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATAAAATTTTAAAA
TCGTATCAAAATGTAGATATGATATTTGGTACACATAATATTCATCATTTACCAGAAATTTTAGAAGAAGCATACTTATC
TAAAGCAATGGTTGTTGAAGTATGGTCTAAAGAAGGAGACGTTATTGAAAATCTTCCAAAAGTCCGTGAAGGCAACATTA
AAGCATGGGTCAATATTATGTATGGTTGTGATAAGTTTTGTACATATTGTATTGTTCCATTTACAAGAGGTAAAGAACGA
AGCCGTAGACCTGAAGACATTATAGATGAAGTACGTGAACTTGCTCGTGAAGGTTACAAAGAAATAACGCTTTTAGGTCA
AAATGTAAATTCTTATGGTAAAGATTTACAGGATATAGAATATGACTTAGGAGATCTTTTACAAGCAATTTCTAAAATAG
CGATTCCAAGAGTTCGTTTCACAACAAGTCATCCTTGGGACTTTACAGATCACATGATTGATGTTATTTCAGAGGGTGGT
AATATCGTTCCTCATATCCACTTGCCAGTTCAATCTGGAAATAATGCAGTATTAAAAATAATGGGTAGAAAATATACACG
AGAAAGTTATTTGGATTTAGTAAAACGAATCAAAGATAGAATTCCTAATGTAGCATTAACTACAGATATTATTGTAGGGT
ATCCAAATGAATCAGAGGAACAATTTGAAGAAACTTTAACTCTGTATGATGAAGTTGGTTTTGAACATGCATATACGTAC
TTGTATTCACAACGTGATGGTACGCCTGCTGCTAAAATGAAAGATAATGTACCTTTAAATGTCAAAAAGGAACGATTGCA
ACGTTTGAATAAAAAAGTTGGTCATTATTCACAAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTG
AAGGTAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGCTAGTTAATTTCAAAGCACCTAAA
GAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGT
AGAGCCGGAAATGGTGATTCAATAA

Upstream 100 bases:

>100_bases
CTTGTTAGAGATGACGTATAACTTTAACTGTTTTTGCACTTTATAGTTAAATTTAATATAATTATTAAATGATACGGGCA
AATAGAAAGGATTTTGTAAA

Downstream 100 bases:

>100_bases
ATGTATAATAAAGATGACGTGTTGAAACAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATA
TCAACAAATTGAAGCACAGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFVKEVEPEMVIQ

Sequences:

>Translated_514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFVKEVEPEMVIQ
>Mature_514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFVKEVEPEMVIQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=443, Percent_Identity=37.6975169300226, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI28872784, Length=443, Percent_Identity=37.6975169300226, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI93277076, Length=445, Percent_Identity=28.5393258426966, Blast_Score=179, Evalue=5e-45,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=41.3092550790068, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1787057, Length=462, Percent_Identity=25.7575757575758, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=32.9809725158562, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=364, Percent_Identity=27.1978021978022, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=37.7593360995851, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=30.2832244008715, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_STAA1 (A7X1T3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441872.1
- ProteinModelPortal:   A7X1T3
- SMR:   A7X1T3
- STRING:   A7X1T3
- EnsemblBacteria:   EBSTAT00000004361
- GeneID:   5560623
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1282
- eggNOG:   COG0621
- GeneTree:   EBGT00050000025066
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14327
- BioCyc:   SAUR418127:SAHV_1282-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 58918; Mature: 58918

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCHH
YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCH
NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
HHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCHHEEHHHHHHHHHHHHHEEEECCCCCCC
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR
ECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLN
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCHHHCCCCCCCC
VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
CHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCEEECCCCH
EMIGKLVEVRIDEAKQYSLNGSFVKEVEPEMVIQ
HHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCHH
YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCH
NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
HHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCHHEEHHHHHHHHHHHHHEEEECCCCCCC
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR
ECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLN
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCHHHCCCCCCCC
VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
CHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCEEECCCCH
EMIGKLVEVRIDEAKQYSLNGSFVKEVEPEMVIQ
HHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA