BacMap

Definition	Staphylococcus aureus subsp. aureus N315, complete genome.
Accession	NC_002745
Length	2,814,816

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The map label for this gene is yejF [C]

Identifier: 15926662

GI number: 15926662

Start: 1053905

End: 1055305

Strand: Reverse

Name: yejF [C]

Synonym: SA0928

Alternate gene names: 15926662

Gene position: 1055305-1053905 (Counterclockwise)

Preceding gene: 15926663

Following gene: 15926661

Centisome position: 37.49

GC content: 33.26

Gene sequence:

>1401_bases
GTGTTAAAAGTAAGTGATTTACGATTAAAATATCCAAATGGTCAGCGTAAAATTTTCGATCATTTAAATATCACTATTCA
AGACAAAGAAAAAGTACTTTTACTCGGTCCTTCTGGTTGCGGTAAAAGTACACTTCTGAATGTATTAAGTGGTATTGTTC
CTAATTTAATTGAATTACCTATGAAATATGATGAACTAATCGTTGACCCATTAAGTGGCGTTATTTTCCAAGACCCTGAT
AGCCAGTTTTGTATGCCAAAAGTATACGAAGAACTTGCATTCGTTTTAGAAAATAGACAAGTACCACGTGAAGACATGGA
TGCGTTAATTATCAATGCTTTAAATATGGTCAATTTAAATGTTACCCCTGAAACGTATATCAAAGATTTAAGTGGCGGGA
TGAAACAGAAATTGGCAATTGTTGAAACCATTCTTCAACAATCAAAAACATTGTTTTTAGATGAACCGACAGCAATGTTA
GATGTTCAAGCAACAGAAGATTTATGGACTAAACTAATTGAACTTTGGGAAGATCAAACTGTTGTAATCGTTGAACATAA
AGTTAAACACATCTGGAATCATGTCGACCGCGTCATTTTGATGGATTATAACGGAAATATCATTGCCGATGAATGTCCTG
AAATCATATTACAGAAGTATGTTCATTTACTCAGTGAATATGGTGTGTGGCATCCACGTGCATGGGAATTCGCACCAAGT
CGTGTTGACTTTCCAACAACAAACTCACACTTATTACAATTTAAAAATGGACGTATTATTCGCGGTAAATCAACATTGCT
CTCATTCTCAGATTTAGAAATTGGTCTAGGTGAGTGGATTACAATTACAGGGGCAAATGGTAGTGGTAAAACAACCTTGC
TTGAATCAATTATGCAATTGATTAAATATCAAGGTGATGTTTATTTTGAAAATCAGCGTTTAACAAAAATTAAACATGCA
GCAAAACACATGTACCTAGTTTATCAAAACCCAGAATTACAATTTATAACAAATTCGGTTTATGATGAAATTAACATTCA
TTTTAATCACCTTTCTAAAGATCAAAGTGATGATGAAACGATACAACTTTTAAAACTTTTAGATTTACAAAATGTAAAAG
ATCAACATCCTTATGAGTTGTCTATTGGTCAAAAACGACGCCTTAGCGTAGCTACCGCACTAAGTTCTAAAGCTGATATT
ATCTTTTTAGATGAACCGACATTTGGACTTGATAGCCATAATACATTCCAGTTGATCAAACTTTTCCAAAAGCGAATTAA
TTTGGGACAAAGTATTGTCATGGTTACACATGACGATGAAATAATTGAACGTTATCCATCAAGAAGACTTAAAATTTCAG
ATGGTGCGTTACTAGATTGTGATGGTGATACGAATGTATGA

Upstream 100 bases:

>100_bases
CTTACCTTTTAGTAAAAGCTTTAGATAAGACAGGCGTAACAAAATTATTTAGACCAGCTGCTAAAGAAGATTACGACAAC
TTATAAGAGGAGCATTCAGA

Downstream 100 bases:

>100_bases
GCAATGGAAAGTTCGGCAATCATTTGTTGATGATGTCAACATTATAACGAAACTATTTCTTGGAATTATACTATTTTTCT
TTATTATATTTGTGCATAAT

Product: hypothetical protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKSTLLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPD
SQFCMPKVYEELAFVLENRQVPREDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQKYVHLLSEYGVWHPRAWEFAPS
RVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGLGEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHA
AKHMYLVYQNPELQFITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVATALSSKADI
IFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRLKISDGALLDCDGDTNV

Sequences:

>Translated_466_residues
MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKSTLLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPD
SQFCMPKVYEELAFVLENRQVPREDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQKYVHLLSEYGVWHPRAWEFAPS
RVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGLGEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHA
AKHMYLVYQNPELQFITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVATALSSKADI
IFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRLKISDGALLDCDGDTNV
>Mature_466_residues
MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKSTLLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPD
SQFCMPKVYEELAFVLENRQVPREDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQKYVHLLSEYGVWHPRAWEFAPS
RVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGLGEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHA
AKHMYLVYQNPELQFITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVATALSSKADI
IFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRLKISDGALLDCDGDTNV

Specific function: Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system [H]

COG id: COG1122

COG function: function code P; ABC-type cobalt transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transporter domains [H]

Homologues:

Organism=Homo sapiens, GI30795238, Length=205, Percent_Identity=28.780487804878, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI27881501, Length=205, Percent_Identity=28.780487804878, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI46592964, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI46592956, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI46592971, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI46592978, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI46592898, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI46592915, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI116734710, Length=214, Percent_Identity=26.6355140186916, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI42741659, Length=248, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788506, Length=506, Percent_Identity=25.098814229249, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI145693107, Length=531, Percent_Identity=23.9171374764595, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786563, Length=228, Percent_Identity=28.0701754385965, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787029, Length=226, Percent_Identity=29.2035398230088, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787792, Length=465, Percent_Identity=22.5806451612903, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081834, Length=212, Percent_Identity=31.1320754716981, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786398, Length=213, Percent_Identity=28.169014084507, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081709, Length=208, Percent_Identity=27.8846153846154, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787105, Length=198, Percent_Identity=28.2828282828283, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787370, Length=224, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789991, Length=488, Percent_Identity=23.5655737704918, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788761, Length=213, Percent_Identity=29.1079812206573, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789873, Length=190, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI48994943, Length=436, Percent_Identity=22.4770642201835, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789672, Length=219, Percent_Identity=27.3972602739726, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788225, Length=209, Percent_Identity=27.7511961722488, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790525, Length=460, Percent_Identity=23.2608695652174, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082267, Length=183, Percent_Identity=26.775956284153, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786703, Length=205, Percent_Identity=30.7317073170732, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790162, Length=226, Percent_Identity=30.5309734513274, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081791, Length=235, Percent_Identity=26.8085106382979, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994997, Length=209, Percent_Identity=30.1435406698565, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790467, Length=174, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787712, Length=212, Percent_Identity=27.3584905660377, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787089, Length=203, Percent_Identity=26.6009852216749, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788450, Length=170, Percent_Identity=31.7647058823529, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789891, Length=197, Percent_Identity=26.9035532994924, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788472, Length=499, Percent_Identity=21.2424849699399, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787758, Length=188, Percent_Identity=28.7234042553192, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786698, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082064, Length=194, Percent_Identity=27.8350515463918, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789963, Length=183, Percent_Identity=26.775956284153, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994883, Length=217, Percent_Identity=22.5806451612903, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787164, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787500, Length=187, Percent_Identity=24.5989304812834, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786803, Length=244, Percent_Identity=23.7704918032787, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789962, Length=179, Percent_Identity=25.6983240223464, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787112, Length=185, Percent_Identity=25.4054054054054, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788165, Length=193, Percent_Identity=26.9430051813472, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786872, Length=188, Percent_Identity=23.936170212766, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789032, Length=184, Percent_Identity=26.6304347826087, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789593, Length=222, Percent_Identity=27.4774774774775, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17569145, Length=235, Percent_Identity=28.5106382978723, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17555800, Length=446, Percent_Identity=24.4394618834081, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193202349, Length=245, Percent_Identity=26.1224489795918, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193211017, Length=232, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=232, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567265, Length=222, Percent_Identity=28.8288288288288, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=232, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17569143, Length=225, Percent_Identity=27.1111111111111, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558664, Length=235, Percent_Identity=24.6808510638298, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=197, Percent_Identity=27.9187817258883, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17541710, Length=241, Percent_Identity=26.1410788381743, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115535101, Length=218, Percent_Identity=27.5229357798165, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71985417, Length=168, Percent_Identity=27.9761904761905, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6324498, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28574150, Length=187, Percent_Identity=29.9465240641711, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI116007328, Length=187, Percent_Identity=29.9465240641711, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI85726357, Length=216, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581191, Length=224, Percent_Identity=29.0178571428571, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581189, Length=224, Percent_Identity=29.0178571428571, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581193, Length=224, Percent_Identity=29.0178571428571, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581195, Length=224, Percent_Identity=29.0178571428571, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221500365, Length=243, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI116007184, Length=243, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI85725262, Length=222, Percent_Identity=26.1261261261261, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574744, Length=173, Percent_Identity=27.1676300578035, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 53330; Mature: 53330

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKSTLLNVLSGIVPNLIELP
CCCCCCEEEECCCCCCEEHHHEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
MKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVPREDMDALIINALNMVNLN
CCHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEC
VTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAMLDVQATEDLWTKLIELWEDQT
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHCCCE
VVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQKYVHLLSEYGVWHPRAWEFAPS
EEEEHHHHHHHHHHCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGLGEWITITGANGSGKTTLLESIMQL
CCCCCCCCCEEEEECCCEEEECCHHEEEECCCEECCCCEEEEECCCCCCHHHHHHHHHHH
IKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQFITNSVYDEINIHFNHLSKDQSDDET
HHHCCCEEECCCHHHHHHHHHHEEEEEEECCCEEEEEHHHCCEEEEEEEECCCCCCCHHH
IQLLKLLDLQNVKDQHPYELSIGQKRRLSVATALSSKADIIFLDEPTFGLDSHNTFQLIK
HHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHH
LFQKRINLGQSIVMVTHDDEIIERYPSRRLKISDGALLDCDGDTNV
HHHHHHCCCCEEEEEECCHHHHHHCCCCEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MLKVSDLRLKYPNGQRKIFDHLNITIQDKEKVLLLGPSGCGKSTLLNVLSGIVPNLIELP
CCCCCCEEEECCCCCCEEHHHEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
MKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVPREDMDALIINALNMVNLN
CCHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEC
VTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAMLDVQATEDLWTKLIELWEDQT
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEECHHHHHHHHHHHHCCCE
VVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQKYVHLLSEYGVWHPRAWEFAPS
EEEEHHHHHHHHHHCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGLGEWITITGANGSGKTTLLESIMQL
CCCCCCCCCEEEEECCCEEEECCHHEEEECCCEECCCCEEEEECCCCCCHHHHHHHHHHH
IKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQFITNSVYDEINIHFNHLSKDQSDDET
HHHCCCEEECCCHHHHHHHHHHEEEEEEECCCEEEEEHHHCCEEEEEEEECCCCCCCHHH
IQLLKLLDLQNVKDQHPYELSIGQKRRLSVATALSSKADIIFLDEPTFGLDSHNTFQLIK
HHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHH
LFQKRINLGQSIVMVTHDDEIIERYPSRRLKISDGALLDCDGDTNV
HHHHHHCCCCEEEEEECCHHHHHHCCCCEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]