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The map label for this gene is folD

Identifier: 15926649

GI number: 15926649

Start: 1040543

End: 1041403

Strand: Reverse

Name: folD

Synonym: SA0915

Alternate gene names: 15926649

Gene position: 1041403-1040543 (Counterclockwise)

Preceding gene: 15926661

Following gene: 15926648

Centisome position: 37.0

GC content: 34.26

Gene sequence:

>861_bases
ATGGTTGCTAAAATTTTAGATGGTAAACAAATTGCCAAAGACTACAGACAGGGGTTACAAGATCAAGTTGAAGCGCTAAA
AGAAAAGGGTTTTACACCTAAATTATCCGTTATATTAGTTGGTAATGATGGCGCTAGTCAAAGTTATGTTAGATCAAAAA
AGAAAGCAGCTGAAAAAATTGGTATGATTTCAGAAATCGTACATTTGGAAGAAACAGCTACTGAAGAAGAAGTATTAAAC
GAACTAAATAGACTAAATAATGATGATTCTGTAAGTGGTATTTTGGTACAAGTACCATTACCAAAACAAGTTAGCGAACA
GAAAATATTAGAAGCAATCAATCCTGAAAAAGATGTGGACGGATTTCATCCAATAAATATAGGGAAATTATATATCGATG
AACAAACTTTTGTACCTTGCACACCGCTCGGCATCATGGAAATATTAAAACATGCTGATATTGATTTAGAAGCTAAAAAT
GCAGTTGTAATTGGACGAAGTCATATTGTCGGACAACCAGTTTCTAAGTTACTACTTCAAAAAAATGCATCAGTAACAAT
CTTACATTCTCGTTCAAAAGATATGGCATCATATTTAAAAGATGCTGATGTCATTGTCAGTGCAGTTGGTAAGCCTAGTT
TAGTAACAAAAGATGTGGTCAAAGAAGGAGCAGTAATTATCGATGTTGGCAATACGCCAGATGAAAATGGCAAATTAAAA
GGTGACGTTGATTATGATGCGGTTAAAGAAATTGCTGGAGCTATTACACCAGTTCCTGGTGGCGTTGGTCCATTAACAAT
TACTATGGTATTAAATAATACTTTGCTTGCAGAAAAAATGCGTCGAGGTATTGATTCGTAA

Upstream 100 bases:

>100_bases
CTTTATTTTGATTTTTTGTTTGAATTTACGTCTTTTGCTATGTAAATTAGTTTTATAAACTAACAAAGTTAGGATATTGA
CAATAGGAGGAGAAGTTTTT

Downstream 100 bases:

>100_bases
AGAGCCTGAGACATAAATCAATGTTCTATGCTCTACAAAGTTATAATGGCAGTAGTTGACTGAACGAAAATTCGCTTGTA
ACAAGCTTTTTTCAATTCTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLN
ELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKN
AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKDVVKEGAVIIDVGNTPDENGKLK
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS

Sequences:

>Translated_286_residues
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLN
ELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKN
AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKDVVKEGAVIIDVGNTPDENGKLK
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS
>Mature_286_residues
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLN
ELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKN
AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKDVVKEGAVIIDVGNTPDENGKLK
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=45.3924914675768, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI222136639, Length=289, Percent_Identity=43.598615916955, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=43.3447098976109, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI36796743, Length=226, Percent_Identity=26.5486725663717, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=46.4028776978417, Blast_Score=253, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=36.9491525423729, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=43.4343434343434, Blast_Score=249, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6319558, Length=292, Percent_Identity=44.1780821917808, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6322933, Length=314, Percent_Identity=28.6624203821656, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=43.1972789115646, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=43.1972789115646, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=41.1971830985916, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=41.1971830985916, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=41.1971830985916, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=41.1971830985916, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_STAA1 (A7X0V3)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441645.1
- ProteinModelPortal:   A7X0V3
- SMR:   A7X0V3
- STRING:   A7X0V3
- EnsemblBacteria:   EBSTAT00000005352
- GeneID:   5561374
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1055
- eggNOG:   COG0190
- GeneTree:   EBGT00050000024338
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK14175
- BioCyc:   SAUR418127:SAHV_1055-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30888; Mature: 30888

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
GMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVD
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKNAVVIGRSHIVGQPVSKLLLQ
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEECCCEEEEECCHHCCCHHHHHHHH
KNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKDVVKEGAVIIDVGNTPDENGKLK
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEE
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS
CCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDGASQSYVRSKKKAAEKI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
GMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPKQVSEQKILEAINPEKDVD
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCC
GFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEAKNAVVIGRSHIVGQPVSKLLLQ
CCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEECCCEEEEECCHHCCCHHHHHHHH
KNASVTILHSRSKDMASYLKDADVIVSAVGKPSLVTKDVVKEGAVIIDVGNTPDENGKLK
CCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCEE
GDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLLAEKMRRGIDS
CCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA