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The map label for this gene is murE

Identifier: 15926606

GI number: 15926606

Start: 992491

End: 993975

Strand: Direct

Name: murE

Synonym: SA0876

Alternate gene names: 15926606

Gene position: 992491-993975 (Clockwise)

Preceding gene: 15926603

Following gene: 15926607

Centisome position: 35.26

GC content: 36.84

Gene sequence:

>1485_bases
TTGGATGCAAGTACGTTGTTTAAGAAAGTAAAAGTAAAGCGTGTATTGGGTTCTTTAGAACAACAAATAGATGATATCAC
TACTGATTCACGTACAGCGAGAGAAGGTAGCATTTTTGTCGCTTCAGTTGGATATACTGTAGACAGTCATAAGTTCTGTC
AAAATGTAGCTGATCAAGGGTGTAAGTTGGTAGTGGTCAATAAAGAACAATCATTACCAGCTAACGTAACACAAGTGGTT
GTGCCGGACACATTAAGAGTAGCTAGTATTCTAGCACACACATTATATGATTATCCGAGTCATCAGTTAGTGACATTTGG
TGTAACGGGTACAAATGGTAAAACTTCTATTGCGACGATGATTCATTTAATTCAAAGAAAGTTACAAAAAAATAGTGCAT
ATTTAGGAACTAATGGTTTCCAAATTAATGAAACAAAGACAAAAGGTGCAAATACGACACCAGAAACAGTTTCTTTAACT
AAGAAAATTAAAGAAGCAGTTGATGCAGGCGCTGAATCTATGACATTAGAAGTATCAAGCCATGGCTTAGTATTAGGACG
ACTGCGAGGCGTTGAATTTGACGTTGCAATATTTTCAAATTTAACACAAGACCATTTAGATTTTCATGGCACAATGGAAG
CATACGGACACGCGAAGTCTTTATTGTTTAGTCAATTAGGTGAAGATTTGTCGAAAGAAAAGTATGTCGTGTTAAACAAT
GACGATTCATTTTCTGAGTATTTAAGAACAGTGACGCCTTATGAAGTATTTAGTTATGGAATTGATGAGGAAGCCCAATT
TATGGCTAAAAATATTCAAGAATCTTTACAAGGTGTCAGCTTTGATTTTGTAACGCCTTTTGGAACTTACCCAGTAAAAT
CGCCTTATGTTGGTAAGTTTAATATTTCTAATATTATGGCGGCAATGATTGCGGTGTGGAGTAAAGGTACATCTTTAGAA
ACGATTATTAAAGCTGTTGAAAATTTAGAACCTGTTGAAGGGCGATTAGAAGTTTTAGATCCTTCGTTACCTATTGATTT
AATTATCGATTATGCACATACAGCTGATGGTATGAACAAATTAATCGATGCAGTACAGCCTTTTGTAAAGCAAAAGTTGA
TATTTTTAGTTGGTATGGCAGGCGAACGTGATTTAACTAAAACGCCTGAAATGGGGCGAGTTGCCTGTCGTGCAGATTAT
GTCATTTTCACACCGGATAATCCGGCAAATGATGACCCGAAAATGTTAACGGCAGAATTAGCCAAAGGTGCAACACATCA
AAACTATATTGAATTTGATGATCGTGCAGAAGGGATAAAACATGCAATTGACATAGCTGAGCCTGGGGATACTGTCGTTT
TAGCATCAAAAGGAAGAGAACCATATCAAATCATGCCAGGGCATATTAAGGTGCCACATCGAGATGATTTAATTGGCCTT
GAAGCAGCTTACAAAAAGTTCGGTGGTGGCCCTGTTGATCAATAA

Upstream 100 bases:

>100_bases
GAAAATGCTGATTAGTAGGATATAACAGTGAAATTATAAATTTATTAACATCAACAAAACGTGTATAATAAACATATTGT
AGAAAAAGGAGCGGTTCAGT

Downstream 100 bases:

>100_bases
AAGATTTATTGATGAAGGTAAAACTATTGATGTTTATTTATTCGAAGCATTAAATAACCAGATAATCATTGCTATACCAG
ATTGGTTTTGGTCATATCAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVV
VPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLE
TIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADY
VIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
EAAYKKFGGGPVDQ

Sequences:

>Translated_494_residues
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVV
VPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLE
TIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADY
VIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
EAAYKKFGGGPVDQ
>Mature_494_residues
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVV
VPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLE
TIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADY
VIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
EAAYKKFGGGPVDQ

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=462, Percent_Identity=30.952380952381, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786274, Length=318, Percent_Identity=27.0440251572327, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_STAA1 (A7X0P6)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441602.1
- ProteinModelPortal:   A7X0P6
- SMR:   A7X0P6
- STRING:   A7X0P6
- EnsemblBacteria:   EBSTAT00000004890
- GeneID:   5560555
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1012
- eggNOG:   COG0769
- GeneTree:   EBGT00050000023808
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   PRK14022
- BioCyc:   SAUR418127:SAHV_1012-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.7

Molecular weight: Translated: 54233; Mature: 54233

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 179-179 BINDING 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
CEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
HHHHHHHHHCCCCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
CCEEEEEECCCEEEHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
CCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEECHHHHHHHHH
LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECEEEECCCCCCCCCHHHHHHHH
AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
HCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCEEECCCCCCCCHH
EAAYKKFGGGPVDQ
HHHHHHHCCCCCCC
>Mature Secondary Structure
MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVGYTVDSHKFCQNVADQG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCC
CKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQLVTFGVTGTNGKTSIATM
CEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
IHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLTKKIKEAVDAGAESMTLEVSS
HHHHHHHHHCCCCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECC
HGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHAKSLLFSQLGEDLSKEKYVVLNN
CCEEEEEECCCEEEHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
DDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESLQGVSFDFVTPFGTYPVKSPYVGKF
CCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
NISNIMAAMIAVWSKGTSLETIIKAVENLEPVEGRLEVLDPSLPIDLIIDYAHTADGMNK
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEECHHHHHHHHH
LIDAVQPFVKQKLIFLVGMAGERDLTKTPEMGRVACRADYVIFTPDNPANDDPKMLTAEL
HHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEECEEEECCCCCCCCCHHHHHHHH
AKGATHQNYIEFDDRAEGIKHAIDIAEPGDTVVLASKGREPYQIMPGHIKVPHRDDLIGL
HCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCEEECCCCCCCCHH
EAAYKKFGGGPVDQ
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA