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The map label for this gene is mnhA

Identifier: 15926541

GI number: 15926541

Start: 915725

End: 918130

Strand: Reverse

Name: mnhA

Synonym: SA0813

Alternate gene names: 15926541

Gene position: 918130-915725 (Counterclockwise)

Preceding gene: 15926542

Following gene: 15926540

Centisome position: 32.62

GC content: 33.54

Gene sequence:

>2406_bases
GTGAGTTTGTTACATATTGCGGTGATATTACCGTTAATATTCGCACTCATTATTCCTATCCTGTATAGATTTTTTAAACG
TATACATTTAGGATGGTTCGTATTACCTGTCCCTATCGTCATATTTATTTATATGCTTACATTAATTAAAACGACGATGT
CAGGTAATACTGTAATGAAAACCTTAAATTGGATGCCTCATTTTGGTATGAACTTTGATTTATATCTTGATGGCTTAGGT
TTACTCTTTAGTTTGCTTATTTCAGGAATCGGCAGTTTAGTAGTATTATATTCAATCGGCTACTTAAGTAAGTCAGAACA
ACTAGGGAATTTTTATTGTTATTTATTACTATTTATGGGTGCAATGTTAGGTGTCGTACTTTCAGATAATGTTATTATTC
TATATTTATTCTGGGAGCTAACGTCATTTTCTAGTTTTTTACTTATTTCATTCTGGCGCGAACGACAAGCATCTATATAT
GGTGCTCAAAAATCGCTCATAATCACAGTATTTGGTGGTCTATCATTATTAGGTGGTATCATCTTACTCGCTATACCAAC
ACAATCATTTAGCATTCAGTACATGATTCAACATGCTTCTGAAATTCAAAATAGCCCATTCTTCATTTTTGCGATGATTT
TAATCATGATTGGTGCTTTTACAAAATCTGCTCAGTTTCCATTTTATATTTGGCTACCAGATGCAATGGAAGCACCTACA
CCAGTCAGTGCTTATCTTCATTCAGCAACAATGGTTAAAGCAGGGTTATATTTAATCGCTCGAATGACACCAATCTTCGC
AGCATCGCAAGGTTGGGTATGGACTGTCACACTCGTTGGTTTAATTACGTTATTCTGGGCTTCGCTAAACGCAACAAAAC
AACAAGACTTGAAAGGTATACTGGCATTTTCAACAGTATCCCAATTAGGTATGATTATGGCGATGCTTGGAATCGGTGCC
ATTAGTTATCACTATCAAGGCGATGATAGCAAAATATATGCTGCTGCATTTACGGCTGCAATATTCCATTTAATCAACCA
TGCAACATTTAAAGGTGCCCTATTTATGATTACAGGTGCAGTGGATCATTCTACTGGTACACGTGATGTTAAGAAATTAG
GTGGCTTACTAACAATTATGCCTATTTCATTTACAATCACTGTTATTACAGCATTAAGTATGGCTGGTGTACCACCGTTC
AATGGTTTCTTATCAAAAGAATCATTTTTAGAAACAACATTTACAGCAAGCCAAGCAAATTTATTCAGTGTTGATACATT
AGGCTACCTATTCCCAATCATTGGTATAGTGGGCAGTGTCTTCACATTTGTATATTCAATTAAATTTATAATGCATATAT
TTTTCGGACAATATAAACCTGAACAATTACCTAAAAAAGCACACGAAGTATCAATATTAATGCTTTTATCACCAGCTATT
TTAGCTACATTAGTAATTGTATTGGGTTTATTCCCTGGCATTTTAACTAATTCGATTATTGAACCAGCTACATCTTCGAT
TAATCATACGGTAATTGATGATGTTGAATTCCATATGTTCCATGGATTAACGCCTGCCTTTTTATCAACATTAGTGATTT
ATATTTTAGGTATCTTACTAATTGTGACATTTAGTTATTGGGTGAAATTGTTGCAACGACAACCTGGTAAACTAACATTC
AATTATTGGTACAACAGAAGTGCAAATGTTATTCCAAACTACTCTGAAAAGATGACGAATAGCTATGTTACCGATTATTC
ACGTAATAATTTAGTTATCATTTTTGGTGCATTAATTTTATTAACATTTGTCACTATATTCTCAGTGCCTTTCAACATTA
ATTTTAAAGACGTTAGTCCGATTAGAATTTTCGAAGTTTGTATCGTAATTTTATTATTAAGTGCTGCCTTTTTAATTCTT
TTCGCTAAATCAAGATTATTTAGCATTATTATGTTAAGTGCAGTTGGATATGCTGTGTCTGTATTGTTTATATTCTTTAA
AGCACCCGACTTAGCATTGACACAGTTTGTCGTTGAATCTATTTCAACAGCATTGTTCTTATTATGCTTCTACCACTTAC
CAAATTTAAATCGTTATAATGAAAAGCGCTCATTCCAGCTTACTAATGCTTTAATTGCTGGTGGTGTTGGTTTGTCCGTC
ATTATTATCGGTTTAATTGCATACGGAAATAGACATTTTGAATCGATATCTAAGTTTTATCAAGAGCACGTTTATGATTT
AGCTCACGGTAAAAACATGGTAAACGTCATACTTGTTGATTTCCGTGGTATGGATACTTTATTCGAGTCATCTGTGCTTG
GTATCGCAGGATTAGCTGTATATACAATGATTAAATTACGTAAAAAGCGTCAAACGCAAGGTAACGAGGTGAAAAATCAT
GAATAG

Upstream 100 bases:

>100_bases
TTCTGAAAATAAAGCACAACAAGGAGTGTGTACGTTTTTCAGAACTATATCATTCATGTATAATGAATAACATAAATATT
TTTTAAAGGAGGACAACATA

Downstream 100 bases:

>100_bases
ACAACAAAATGATCTAATTTTGCAATTTGCTGCAGTTATTATCTTTTTCATGGTAATGGTCTTTGGTTTTTCACTTTTCT
TAGCAGGACATTATACACCT

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mnh complex subunit A1

Number of amino acids: Translated: 801; Mature: 800

Protein sequence:

>801_residues
MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLG
LLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT
PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGA
ISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPF
NGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI
LATLVIVLGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTF
NYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLIL
FAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV
IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNH
E

Sequences:

>Translated_801_residues
MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLG
LLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT
PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGA
ISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPF
NGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI
LATLVIVLGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTF
NYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLIL
FAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV
IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNH
E
>Mature_800_residues
SLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGL
LFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYG
AQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPTP
VSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAI
SYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFN
GFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAIL
ATLVIVLGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFN
YWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILF
AKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVI
IIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVKNHE

Specific function: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family

Homologues:

Organism=Homo sapiens, GI251831117, Length=473, Percent_Identity=29.8097251585624, Blast_Score=178, Evalue=2e-44,
Organism=Escherichia coli, GI1788614, Length=522, Percent_Identity=30.4597701149425, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788829, Length=477, Percent_Identity=32.4947589098532, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=32.4022346368715, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788827, Length=445, Percent_Identity=26.7415730337079, Blast_Score=126, Evalue=6e-30,
Organism=Escherichia coli, GI1788613, Length=466, Percent_Identity=26.1802575107296, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI2367154, Length=458, Percent_Identity=24.0174672489083, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI145693160, Length=395, Percent_Identity=23.0379746835443, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNHA1_STAA1 (A7X0G4)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441537.1
- STRING:   A7X0G4
- EnsemblBacteria:   EBSTAT00000004686
- GeneID:   5561450
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0947
- eggNOG:   COG1009
- GeneTree:   EBGT00050000024644
- HOGENOM:   HBG672487
- OMA:   SWGDRPK
- ProtClustDB:   PRK12645
- BioCyc:   SAUR418127:SAHV_0947-MONOMER
- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393
- PRINTS:   PR01435
- TIGRFAMs:   TIGR00940

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 89363; Mature: 89232

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xdac0ec4)-; HASH(0xc899eb4)-; HASH(0xcb06dbc)-; HASH(0xd23a750)-; HASH(0xcc0c734)-; HASH(0xd287d70)-; HASH(0xc555a48)-; HASH(0xd4bc8f8)-; HASH(0xd1b10f4)-; HASH(0xc9989d8)-; HASH(0xd046334)-; HASH(0xc0e3c14)-; HASH(0xc7fed58)-; HASH(0xcc35850)-; HASH(0xc50dfd0)-; HASH(0xd4a90d0)-; HASH(0xd3c5e90)-; HASH(0xdda9ce0)-; HASH(0xd7ec398)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMG
HHHCCCCCCCCHHHEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQKSLIITVFGGLSLLGGI
HHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
ILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT
HEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
LAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGA
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCEEEEEEEC
VDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFNGFLSKESFLETTFTASQAN
CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
LATLVIVLGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
IVTFSYWVKLLQRQPGKLTFNYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALIL
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHH
LTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHH
IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
YTMIKLRKKRQTQGNEVKNHE
HHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure 
SLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIYMLTLIKTTMSGNTVMK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYLSKSEQLGNFYCYLLLFMG
HHHCCCCCCCCHHHEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIYGAQKSLIITVFGGLSLLGGI
HHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
ILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIGAFTKSAQFPFYIWLPDAMEAPT
HEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTVTLVGLITLFWASLNATKQQDLKGI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
LAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYAAAFTAAIFHLINHATFKGALFMITGA
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCEEEEEEEC
VDHSTGTRDVKKLGGLLTIMPISFTITVITALSMAGVPPFNGFLSKESFLETTFTASQAN
CCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LFSVDTLGYLFPIIGIVGSVFTFVYSIKFIMHIFFGQYKPEQLPKKAHEVSILMLLSPAI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
LATLVIVLGLFPGILTNSIIEPATSSINHTVIDDVEFHMFHGLTPAFLSTLVIYILGILL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
IVTFSYWVKLLQRQPGKLTFNYWYNRSANVIPNYSEKMTNSYVTDYSRNNLVIIFGALIL
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHH
LTFVTIFSVPFNINFKDVSPIRIFEVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFIFFKAPDLALTQFVVESISTALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHH
IIIGLIAYGNRHFESISKFYQEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
YTMIKLRKKRQTQGNEVKNHE
HHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA