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The map label for this gene is mnhD2 [H]

Identifier: 15926302

GI number: 15926302

Start: 673120

End: 674616

Strand: Direct

Name: mnhD2 [H]

Synonym: SA0581

Alternate gene names: 15926302

Gene position: 673120-674616 (Clockwise)

Preceding gene: 15926301

Following gene: 15926303

Centisome position: 23.91

GC content: 33.4

Gene sequence:

>1497_bases
ATGCTAAGTAACTTATTGATTTTACCAATGTTATTACCATTCCTTTGTGCCTTAATCCTTGTATTTTTAAAAAATAATGA
TCGTATTTCTAAATATTTATACTTAGGTACAATGACTATCACCACAATTATTTCATTAATGCTATTAATTTATGTTCAGC
GTCACCGTCCAATTACGCTAGACTTTGGAGGATGGTCAGCGCCCTTTGGTATACAGTTTTTAGGAGATTCTTTAAGTTTG
ATTATGGTTACAACCGCTTCGTTTGTGATTACTTTAATTATGGCATACGGATTTGGGCGTGGCGAACATAAAGCAAATCG
TTATCACTTGCCATCGTTCATATTATTTTTAAGTGTTGGCGTGATAGGCTCTTTTCTAACATCAGATTTATTTAATTTAT
ACGTCATGTTTGAAATTATGTTACTAGCGTCATTTGTACTCATTACACTTGGACAATCTGTAGAACAATTACGTGCTGCA
ATTATTTATGTTGTCTTGAATATTATTGGTTCATGGCTATTCTTATTAGGTATAGGTTTACTTTATAAAACAGTAGGTAC
ATTAAACTTTTCACATATTGCAATGCGTTTGAATGACATGGGAGATAATCGCACTGTTACAATGATTTCATTAATCTTCT
TAGTCGCATTTAGTGCGAAAGCAGCGCTGGTCCTTTTTATGTGGCTACCCAAAGCCTACGCTGTGTTAAATACTGAGCTT
GCAGCATTATTTGCAGCGTTAATGACCAAAGTAGGGGCCTATGCATTAATTCGATTCTTCACTTTACTATTTGATCAACA
TAATGATCTCATACATCCATTGCTAGCAACTATGGCTGCTATAACTATGGTCATCGGCGCTATAGGTGTCATTGCTTATA
AAGATATTAAAAAGATTGCAGCTTACCAAGTCATAATCTCAATAGGATTTATCATTTTAGGTTTAGGAACAAACACGTTT
GCAGGTATTAATGGTGCAATATTTTATTTGGTAAATGACATTGTTGTAAAAACATTGCTATTCTTTATTATTGGTAGTTT
AGTTTACATTACAGGCTATCGACAATATCAATATTTGAATGGCTTAGCTAAAAAAGAACCTTTATTTGGAGTTGCGTTTA
TTATAATGATTTTTGCTATTGGCGGCGTGCCTCCATTTAGTGGCTTTCCGGGGAAAGTACTTATTTTCCAAGGTGCATTG
CAAAATGGCAATTATATTGGACTAGCGTTAATGATTATTACTAGTCTAATTGCAATGTACAGTTTATTTAGGATATTTTT
TTATATGTATTTTGGAGATAAAGATGGGGAGGAAGTTAATTTTAAGAAAATCCCGCTATATCGAAAAAGAATTTTAAGTA
TTTTAGTAGTTGTGGTTATCGCAATCGGAATTGCTGCACCTGTTGTGTTAAATGTTACAAGTGATGCAACTGAGTTGAAC
ACGAGTGATCAATTATATCAAAAACTTGTAAATCCGCATTTGAAAGGAGAGGACTAA

Upstream 100 bases:

>100_bases
GTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTTATAGAACTTATAAAGTAACAAAAGAAGATGAAATTGA
AGGCCTAAGGGGGGAAGATG

Downstream 100 bases:

>100_bases
ATGAATCAAATAGTTTTAAATATTATCATTGCATTCTTATGGGTATTATTTCAAGATGAAGATCATTTTAAATTCTCGAC
TTTCTTTTCTGGATATCTAA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: Mrp complex subunit D2; Putative NADH-ubiquinone oxidoreductase subunit mnhD2 [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSL
IMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTF
AGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGAL
QNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
TSDQLYQKLVNPHLKGED

Sequences:

>Translated_498_residues
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSL
IMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTF
AGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGAL
QNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
TSDQLYQKLVNPHLKGED
>Mature_498_residues
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSL
IMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTF
AGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGAL
QNGNYIGLALMIITSLIAMYSLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
TSDQLYQKLVNPHLKGED

Specific function: Expression of the mnh2 operon in E.coli is not able to catalyze Na(+)Li(+)/H(+) antiport. It does however confer higher growth rates than the control strain at up to pH 9.5. The operon may encode an NADH-ubiquinone oxidoreductase [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=356, Percent_Identity=25.561797752809, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI251831116, Length=347, Percent_Identity=23.342939481268, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788829, Length=477, Percent_Identity=28.7211740041929, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788827, Length=383, Percent_Identity=24.2819843342037, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI2367154, Length=394, Percent_Identity=24.8730964467005, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788614, Length=502, Percent_Identity=24.7011952191235, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788613, Length=423, Percent_Identity=24.822695035461, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788831, Length=388, Percent_Identity=23.1958762886598, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55188; Mature: 55188

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTIISLMLLIYVQRHRPITL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
DFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGEHKANRYHLPSFILFLSVG
ECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAAIIYVVLNIIGSWLFLLGIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFSAKAALVLFMWLPKAYAVLNTEL
HHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLATMAAITMVIGAIGVIAYKDIKKIA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYQVIISIGFIILGLGTNTFAGINGAIFYLVNDIVVKTLLFFIIGSLVYITGYRQYQYLN
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGKVLIFQGALQNGNYIGLALMIITSLIAMY
CHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
SLFRIFFYMYFGDKDGEEVNFKKIPLYRKRILSILVVVVIAIGIAAPVVLNVTSDATELN
HHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCC
TSDQLYQKLVNPHLKGED
CHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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CHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA