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The map label for this gene is pdxK

Identifier: 15926258

GI number: 15926258

Start: 631344

End: 632174

Strand: Reverse

Name: pdxK

Synonym: SA0537

Alternate gene names: 15926258

Gene position: 632174-631344 (Counterclockwise)

Preceding gene: 15926263

Following gene: 15926257

Centisome position: 22.46

GC content: 37.91

Gene sequence:

>831_bases
ATGGCTTTAAAGAAAGTTTTAACAATTGCCGGTTCTGACACAAGTGCTGGCGCAGGTATGCAAGCAGATTTGAAAACGTT
CCAAGAATTAGATACGTATGGCATGGTCGCTTTAACTGCCATCGTTACTATGGATAAAGATACATGGTCACACGATGTTA
CACCATTACCAATGGATGTATTTGAAAAACAACTTGAAACGGCTTTATCAATCGGACCTGATGCTATTAAAACAGGTATG
TTAGGTACTGAGGAGATAATCAAACGTGCTGGAGAAGTATATGAGGCATCTAATGCACAATATTTTGTAGTAGATCCTGT
CATGGTTTGTAAAGGCGAAGATGAAGTGCTTAATCCTGGAAATACTGAGGCGATGATTAAATATTTACTACCTAAAGCAA
CGGTAGTGACACCAAACTTATTTGAAGCGGGTCAATTATCAGGATTAGGTAAATTAAATTCAATTGAAGATATGAAAAAG
GCTGCAACAATTATCTTTGATAAAGGCGCACAGCATGTCATCATTAAAGGTGGCAAAGCTTTAGATCAAGATAAATCGTA
TGACTTATACTATGATGGTCAAACATTTTATCAACTAACAACAGACATGTTCCAACAAAGTTATAACCATGGTGCTGGTT
GTACATTTGCTGCGGCAACAACTGCATATTTAGCTAACGGTAAGTCACCGAAAGAAGCTGTGATTAGCGCGAAAGCTTTC
GTCGCTTCTGCTATTAAAAACGGTTGGAAAATGAATGATTTTGTTGGTCCTGTGGATCACGGTGCATACAACCGTATTGA
ACATATCGATGTTGAAGTAACAGAGGTTTAA

Upstream 100 bases:

>100_bases
ATTCATTTCAACAATCATCAAATATTGAATGCTTAATATTTTTCAATTAGGTTAAAAATGATATGATGAATACAATATTA
TTATGTAGGAGTGAAGCTCA

Downstream 100 bases:

>100_bases
CGATTAGGCGGTTCATCTATAAATCAACATTATTAGCCACACCACTTAAATTGGGTATGAACTCAATTTATGTGATGTGG
TTATTTTTTTATAATCATCA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGM
LGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV

Sequences:

>Translated_276_residues
MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGM
LGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
>Mature_275_residues
ALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGML
GTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKA
ATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAFV
ASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=31.5589353612167, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6325378, Length=275, Percent_Identity=28.3636363636364, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=29.1970802919708, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324517, Length=272, Percent_Identity=28.3088235294118, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXK_STAAC (Q5HI96)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185511.1
- ProteinModelPortal:   Q5HI96
- SMR:   Q5HI96
- STRING:   Q5HI96
- EnsemblBacteria:   EBSTAT00000008706
- GeneID:   3238551
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL0626
- TIGR:   SACOL0626
- eggNOG:   COG0351
- GeneTree:   EBGT00050000024460
- HOGENOM:   HBG476673
- OMA:   NPGNTDA
- ProtClustDB:   CLSK884741
- BioCyc:   SAUR93062:SACOL0626-MONOMER
- InterPro:   IPR013749
- InterPro:   IPR004399
- TIGRFAMs:   TIGR00097

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.35

Molecular weight: Translated: 29857; Mature: 29726

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: BINDING 139-139 BINDING 188-188 BINDING 213-213 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDV
CCHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
FEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPG
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHEECCCCEEEEECCEEEECCCCCCCCCC
NTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKA
CHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCEEEEECCCC
LDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
ALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDV
CHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
FEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVCKGEDEVLNPG
HHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHEECCCCEEEEECCEEEECCCCCCCCCC
NTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKA
CHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEECCCCEEEEECCCC
LDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAAATTAYLANGKSPKEAVISAKAF
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA