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The map label for this gene is vraA

Identifier: 15926253

GI number: 15926253

Start: 627340

End: 628716

Strand: Direct

Name: vraA

Synonym: SA0533

Alternate gene names: 15926253

Gene position: 627340-628716 (Clockwise)

Preceding gene: 15926252

Following gene: 15926254

Centisome position: 22.29

GC content: 32.46

Gene sequence:

>1377_bases
ATGAACGTAATTTTAGAACAGTTGAAAACACATACTCAAAATAAACCTAATGACATAGCATTACATATCGATGATGAAAC
AATTACATATAGTCAACTAAATGCCCGCATCACTAGCGCAGTTGAATCTTTGCAGAAATATTCACTTAACCCTGTCGTTG
CTATTAATATGAAATCACCGGTGCAAAGTATTATTTGTTATTTAGCTTTGCATCGTTTACATAAAGTGCCTATGATGATG
GAAGGTAAATGGCAAAGTACTATACATCGTCAATTGATTGAAAAATATGGTATTAAAGATGTAATTGGAGATACAGGTCT
CATGCAGAATATAGACTCACCGATGTTTATTGATTCAACGCAATTACAGCACTACCCCAATTTATTACATATTGGTTTTA
CTTCAGGGACAACTGGACTGCCAAAAGCATATTATCGTGATGAAGATTCATGGTTGGCTTCTTTTGAAGTTAATGAAATG
TTGATGTTAAAAAATGAAAATGCAATAGCAGCCCCTGGACCACTATCGCACTCGTTAACATTATATGCGTTATTGTTTGC
TTTAAGTTCCGGTCGTACTTTTATAGGACAGACCACTTTTCATCCTGAAAAGTTACTTAATCAATGTCATAAAATATCAT
CATACAAAGTTGCTATGTTTCTTGTTCCAACGATGATTAAATCATTATTGTTAGTTTACAACAATGAACATACAATCCAA
TCATTTTTTAGCAGTGGAGATAAGCTGCATTCTTCTATTTTTAAAAAGATAAAAAATCAAGCAAATGACATAAATTTGAT
TGAATTTTTTGGTACATCGGAAACCAGTTTTATCAGCTATAACTTGAATCAGCAAGCACCAGTTGAATCAGTAGGTGTGC
TATTTCCAAATGTGGAATTGAAAACAACGAATCACGATCACAATGGTATAGGAACTATTTGTATAAAAAGTAATATGATG
TTTAGTGGCTATGTAAGTGAACAATGTATAAATAATGATGAATGGTTTGTTACTAATGATAATGGCTATGTAAAAGAGCA
GTATTTATATTTAACGGGACGTCAACAGGATATGTTAATTATTGGTGGTCAAAATATATATCCAGCACATGTTGAACGCC
TTTTAACGCAATCTTCGAGCATTGATGAAGCAATTATCATCGGTATTCCAAATGAGCGTTTTGGTCAAATAGGCGTATTG
CTTTATTCTGGTGATGTGACACTTACACATAAAAATGTAAAACAATTTTTAAAAAAGAAAGTGAAACGCTATGAAATTCC
ATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCAAGTGGTAAAATTGCTAGAGAAAAAATGATGTCGATGTATT
TGAGAGGTGAATTATAA

Upstream 100 bases:

>100_bases
TTGATTTTTATAATAGGCAGGTTTACCTGATAAAAATACTTATTCATTATATAATGTTAACAAGATGTATTTTAAAGTTT
ACATTGAGTGAGGGATATTG

Downstream 100 bases:

>100_bases
TATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGTGGCACTTTAAAACATTTAGAGCCAG
AACAATTGCTTAAACCTTTA

Product: hypothetical protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMM
EGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMM
FSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVL
LYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL

Sequences:

>Translated_458_residues
MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMM
EGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMM
FSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVL
LYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL
>Mature_458_residues
MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMM
EGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMM
FSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVL
LYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI156151445, Length=384, Percent_Identity=26.3020833333333, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI187761345, Length=375, Percent_Identity=22.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI187761343, Length=375, Percent_Identity=22.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=392, Percent_Identity=23.469387755102, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693145, Length=349, Percent_Identity=26.0744985673352, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=23.4421364985163, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=359, Percent_Identity=25.6267409470752, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=359, Percent_Identity=25.6267409470752, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=359, Percent_Identity=25.6267409470752, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=319, Percent_Identity=28.5266457680251, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=355, Percent_Identity=26.4788732394366, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=25.5555555555556, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=20.9923664122137, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=350, Percent_Identity=22.2857142857143, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=358, Percent_Identity=22.9050279329609, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=380, Percent_Identity=22.1052631578947, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=339, Percent_Identity=22.4188790560472, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=156, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581924, Length=360, Percent_Identity=25.8333333333333, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648257, Length=343, Percent_Identity=26.2390670553936, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356947, Length=392, Percent_Identity=22.1938775510204, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24653035, Length=376, Percent_Identity=20.7446808510638, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648260, Length=385, Percent_Identity=24.4155844155844, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17933690, Length=135, Percent_Identity=31.8518518518519, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355181, Length=346, Percent_Identity=24.5664739884393, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358303, Length=315, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24656500, Length=345, Percent_Identity=24.0579710144928, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922652, Length=386, Percent_Identity=22.5388601036269, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=349, Percent_Identity=24.0687679083095, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130357, Length=271, Percent_Identity=21.0332103321033, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VRAA_STAA1 (Q9KWK5)

Other databases:

- EMBL:   AB035449
- EMBL:   AP009324
- RefSeq:   YP_001441163.1
- ProteinModelPortal:   Q9KWK5
- STRING:   Q9KWK5
- EnsemblBacteria:   EBSTAT00000004615
- GeneID:   5558973
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0573
- eggNOG:   COG0318
- GeneTree:   EBGT00050000024490
- HOGENOM:   HBG694025
- OMA:   ENISVMY
- ProtClustDB:   CLSK884736
- BioCyc:   SAUR418127:SAHV_0573-MONOMER
- InterPro:   IPR020845
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 52025; Mature: 52025

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSP
CCHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
VQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDST
HHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCHHHHCCCHHHHCCCCCEEEECH
QLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSHSLT
HHHHCCCEEEEEECCCCCCCCHHHCCCCCCEEEEEECCEEEEEECCCCEECCCCCCHHHH
LYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVEL
HHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHCEEECCEEE
KTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLI
EECCCCCCCEEEEEEECCEEEECCHHHHHCCCCCEEEECCCCEEEEEEEEEECCCCCEEE
IGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVLLYSGDVTLTHKNVKQFLKKK
ECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCEEEEHHHHHHHHHHH
VKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL
HHHCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESLQKYSLNPVVAINMKSP
CCHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
VQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVIGDTGLMQNIDSPMFIDST
HHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCHHHHCCCHHHHCCCCCEEEECH
QLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEMLMLKNENAIAAPGPLSHSLT
HHHHCCCEEEEEECCCCCCCCHHHCCCCCCEEEEEECCEEEEEECCCCEECCCCCCHHHH
LYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVAMFLVPTMIKSLLLVYNNEHTIQ
HHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
SFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETSFISYNLNQQAPVESVGVLFPNVEL
HHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHCEEECCEEE
KTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDEWFVTNDNGYVKEQYLYLTGRQQDMLI
EECCCCCCCEEEEEEECCEEEECCHHHHHCCCCCEEEECCCCEEEEEEEEEECCCCCEEE
IGGQNIYPAHVERLLTQSSSIDEAIIIGIPNERFGQIGVLLYSGDVTLTHKNVKQFLKKK
ECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCEEEEHHHHHHHHHHH
VKRYEIPSMIHHVEKMYYTASGKIAREKMMSMYLRGEL
HHHCCCHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580