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The map label for this gene is metB

Identifier: 15926138

GI number: 15926138

Start: 480605

End: 481747

Strand: Direct

Name: metB

Synonym: SA0419

Alternate gene names: 15926138

Gene position: 480605-481747 (Clockwise)

Preceding gene: 15926137

Following gene: 15926139

Centisome position: 17.07

GC content: 36.13

Gene sequence:

>1143_bases
ATGAATAAGAAAACTAAATTAATTCACGGTGGACACACAACGGACGACTATACAGGTGCAGTTACTACACCAATTTATCA
AACAAGTACATATTTACAAGATGATATTGGTGATTTACGTCAAGGATATGAATATTCTCGTACTGCGAATCCAACAAGAA
GTTCTGTAGAAAGCGTTATTGCGGCATTAGAAAATGGCAAACATGGCTTTGCGTTTAGTTCAGGTGTTGCAGCTATCAGT
GCAGTTGTTATGCTGTTGGACAAAGGTGATCATATTATTTTAAATTCAGATGTATACGGCGGTACTTATCGCGCATTGAC
AAAAGTATTTACACGATTTGGCATTGAAGTGGATTTTGTAGATACAACGCATACAGATTCAATTGTACAAGCGATACGCC
CAACAACAAAGATGTTGTTTATTGAAACACCTTCTAATCCATTATTACGTGTTACTGACATTAAAAAGTCTGCTGAAATT
GCGAAAGAACACGGTTTGATTTCAGTCGTTGATAACACATTTATGACACCTTATTATCAGAATCCATTAGATTTAGGCAT
CGATATTGTCTTGCATTCTGCAACGAAATATTTAGGTGGACATAGTGATGTCGTTGCTGGTTTAGTTGCAACATCGGATG
ACAAGCTTGCAGAACGTTTAGCATTTATTTCAAATTCAACAGGTGGCATTTTAGGACCTCAAGATAGCTATTTACTTGTG
AGGGGTATTAAAACATTAGGTTTACGTATGGAACAAATTAATCGCAGTGTTATTGAAATTATTAAAATGTTACAAGCACA
TCCAGCTGTGCAACAAGTGTTCCATCCAAGTATTGAAAGTCATTTAAATCATGATGTCCATATGGCTCAAGCGGATGGCC
ATACAGGTGTGATTGCATTTGAAGTGAAAAATACAGAAAGTGCCAAACAATTGATTAAAGCAACATCGTATTACACATTA
GCTGAAAGTTTAGGTGCAGTGGAAAGTTTAATTTCAGTACCTGCATTGATGACACATGCATCCATTCCAGCAGATATTCG
AGCTAAAGAAGGTATTACAGACGGACTTGTAAGAATTTCTGTAGGTATTGAAGATACTGAAGATTTAGTCGATGATTTAA
AACAAGCACTAGATACGCTATAA

Upstream 100 bases:

>100_bases
TTAAAAGCGCAATTATCTGAAGGTACGATTGTTGTCGTATTTCCAGATGGTAGCGATCGATATATGTCTAAGCAAATATT
TAATTATGAGGAGAATGATT

Downstream 100 bases:

>100_bases
ATAATAGCAGCACTAGCATATATTTTAAGATCAACTTAAATTGATTTTGAAAATGCTAGTGCTTTTTTGTGCGATGTTAA
TCATTCGTGATTGAGTTATG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAIS
AVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTL
AESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL

Sequences:

>Translated_380_residues
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAIS
AVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTL
AESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL
>Mature_380_residues
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVIAALENGKHGFAFSSGVAAIS
AVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTL
AESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=376, Percent_Identity=45.7446808510638, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI299473759, Length=376, Percent_Identity=41.2234042553192, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI25453487, Length=375, Percent_Identity=39.2, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1790375, Length=377, Percent_Identity=42.4403183023873, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789383, Length=387, Percent_Identity=27.390180878553, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=378, Percent_Identity=45.7671957671958, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17538055, Length=378, Percent_Identity=46.031746031746, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=38.4408602150538, Blast_Score=258, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6319307, Length=382, Percent_Identity=46.8586387434555, Blast_Score=327, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=33.9066339066339, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6321170, Length=297, Percent_Identity=30.976430976431, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=30.5361305361305, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=28.9340101522843, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322590, Length=278, Percent_Identity=26.978417266187, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323557, Length=347, Percent_Identity=25.9365994236311, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=304, Percent_Identity=24.0131578947368, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922554, Length=380, Percent_Identity=47.3684210526316, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1; =4.4.1.2 [H]

Molecular weight: Translated: 41249; Mature: 41249

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVI
CCCCCEEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFV
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
DTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEIAKEHGLISVVDNTFMTPYYQ
CCCCHHHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHHHHHCCEEEEECCCCCCCCCC
NPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
CCHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEHH
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEE
EVKNTESAKQLIKATSYYTLAESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRIS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEE
VGIEDTEDLVDDLKQALDTL
ECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYEYSRTANPTRSSVESVI
CCCCCEEEECCCCCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGTYRALTKVFTRFGIEVDFV
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
DTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEIAKEHGLISVVDNTFMTPYYQ
CCCCHHHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHHHHHCCEEEEECCCCCCCCCC
NPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAERLAFISNSTGGILGPQDSYLLV
CCHHCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEHH
RGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHPSIESHLNHDVHMAQADGHTGVIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEE
EVKNTESAKQLIKATSYYTLAESLGAVESLISVPALMTHASIPADIRAKEGITDGLVRIS
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEE
VGIEDTEDLVDDLKQALDTL
ECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]