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The map label for this gene is ahpF

Identifier: 15926081

GI number: 15926081

Start: 421010

End: 422533

Strand: Reverse

Name: ahpF

Synonym: SA0365

Alternate gene names: 15926081

Gene position: 422533-421010 (Counterclockwise)

Preceding gene: 15926082

Following gene: 15926080

Centisome position: 15.01

GC content: 37.99

Gene sequence:

>1524_bases
ATGCTTAATGCTGATTTAAAACAACAACTTAAACAATTATTAGAACTAATGGAGGGCAACGTTGAATTCGTTGCCAGCCT
TGGTTCAGATGAAAAATCTAAGGAACTCAAAGAATTATTAACAGAAATTTCTGATATGTCACCTAGACTTTCACTTTCTG
AAAAGTCTTTAAAACGTACACCAAGTTTCTCAGTCAACCGTCCTGGCGAAGAAACAGGTGTAACATTTGCAGGAATTCCA
TTAGGTCACGAGTTTAACTCACTTGTTTTAGCAATTTTACAGGTTAGTGGTCGTGCACCTAAAGAAAAACAGTCAATTAT
TGACCAAATTAAAAACTTAGAAGGTTCATTCCATTTTGAAACATTCATTAGTTTAACGTGTCAAAAATGTCCTGATGTCG
TTCAAGCACTTAACTTAATGAGTGTGATCAACCCTAACATCACGCATTCTATGATTGATGGTGCAGTGTTCCGTGAAGAA
TCTGAAAACATCATGGCAGTCCCTGCTGTCTTTTTAAACGGCGAAGAATTTGGCAATGGTCGTATGACAATCCAAGATAT
TCTTTCGAAACTAGGCAGTACGGCAGATGCATCTGAGTTTGAAAATAAAGAACCTTATGATGTCTTAATCGTTGGTGGTG
GTCCTGCTAGTGGTAGTGCAGCGATTTACACAGCACGTAAAGGTTTACGTACTGGTATAGTTGCTGATCGTATCGGTGGC
CAAGTTAATGATACTGCTGGTATTGAGAACTTCATTACTGTTAAAGAAACAACTGGTTCTGAATTTTCTTCTAACTTAGC
AGCGCACATTGATCAATATGACATTGATGCAATGACAGGTATACGTGCTACAGATATCGAAAAGACTGACGAAGCAATTA
AAGTTACGTTAGAAAACGGTGCTGTCTTAGAAAGTAAAACAGTCATTATTGCTACTGGTGCAGGTTGGCGTAAGCTAAAC
ATTCCAGGTGAAGAGCAATTGATTAATAAAGGTGTTGCATTCTGCCCTCACTGTGACGGACCTCTATTCGAAAATAAAGA
CGTAGCAGTTATCGGTGGCGGTAACTCTGGGGTTGAAGCAGCAATTGACCTTGCTGGTATCGTTAATCATGTTACATTAT
TCGAATTCGCTAGCGAATTAAAAGCAGACAACGTGTTACAAGATCGTTTACGTTCTTTATCAAATGTTGATATCAAAACA
AATGCCAAAACTACTGAAGTTGTCGGAGAAGACCATGTTACAGGTATACGTTACGAAGACATGAGCACCGGCGAAGAACA
TCTACTTAACTTAGATGGTATCTTTGTTCAAATTGGTTTACTTCCAAACACATCATGGTTAAAAGATGCTGTTGAATTAA
ACGAACGTGGTGAAATTGTTATTGATCGTAACAATAATACTAACGTACCTGGAATATTTGCTGCTGGCGATGTCACAGAT
CAGAAGAACAAACAAATTATCATTTCAATGGGCGCTGGTGCAAATGCAGCATTAAATGCCTTTGACTATATTATCAGAAA
CTAA

Upstream 100 bases:

>100_bases
AAACCCTGGCGAAGTATGCCCAGCTAAATGGGAAGAAGGCGCTAAAACATTGCAACCTGGTTTAGATTTAGTAGGTAAAA
TCTAAGGAGGCATTACAACC

Downstream 100 bases:

>100_bases
AGTTTTCACAGTTTCACTGACAGTATCTTACATTTGTTAAGATGCTGTTATTTTTTTATCCCGACATAAAATCATGTTAT
GATAAACTTAATATATACAA

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEISDMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIP
LGHEFNSLVLAILQVSGRAPKEKQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLN
IPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKT
NAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
QKNKQIIISMGAGANAALNAFDYIIRN

Sequences:

>Translated_507_residues
MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEISDMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIP
LGHEFNSLVLAILQVSGRAPKEKQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLN
IPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKT
NAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
QKNKQIIISMGAGANAALNAFDYIIRN
>Mature_507_residues
MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEISDMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIP
LGHEFNSLVLAILQVSGRAPKEKQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLN
IPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKT
NAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
QKNKQIIISMGAGANAALNAFDYIIRN

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=523, Percent_Identity=54.302103250478, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=34.5161290322581, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=33.4405144694534, Blast_Score=153, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=312, Percent_Identity=32.0512820512821, Blast_Score=149, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 54708; Mature: 54708

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00626 RCC1_2 ; PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEISDMSPRLSLSEKSLKRT
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
PSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKEKQSIIDQIKNLEGSFHFE
CCCCCCCCCHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEHH
TFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREESENIMAVPAVFLNGEEFGNG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCC
RMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
CEEHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHCHHHHHCCC
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENG
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHCCCEECCCCCCCCEEEEEECCC
AVLESKTVIIATGAGWRKLNIPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEA
EEEECCEEEEEECCCEEEECCCCHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCHHH
AIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKTNAKTTEVVGEDHVTGIRYED
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHEECCCCCCCCEEECC
MSTGEEHLLNLDGIFVQIGLLPNTSWLKDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
CCCCHHHEEECCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCC
QKNKQIIISMGAGANAALNAFDYIIRN
CCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEISDMSPRLSLSEKSLKRT
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
PSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKEKQSIIDQIKNLEGSFHFE
CCCCCCCCCHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEHH
TFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREESENIMAVPAVFLNGEEFGNG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCC
RMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
CEEHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHCHHHHHCCC
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENG
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHCCCEECCCCCCCCEEEEEECCC
AVLESKTVIIATGAGWRKLNIPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEA
EEEECCEEEEEECCCEEEECCCCHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCHHH
AIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKTNAKTTEVVGEDHVTGIRYED
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHEECCCCCCCCEEECC
MSTGEEHLLNLDGIFVQIGLLPNTSWLKDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
CCCCHHHEEECCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCC
QKNKQIIISMGAGANAALNAFDYIIRN
CCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA