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The map label for this gene is metE

Identifier: 15926057

GI number: 15926057

Start: 401175

End: 403403

Strand: Reverse

Name: metE

Synonym: SA0344

Alternate gene names: 15926057

Gene position: 403403-401175 (Counterclockwise)

Preceding gene: 15926058

Following gene: 15926056

Centisome position: 14.33

GC content: 34.37

Gene sequence:

>2229_bases
ATGACAACAATTAAAACATCAAACTTAGGATTCCCAAGATTAGGTAGAAAAAGAGAATGGAAAAAAGCCATCGAAAGTTA
TTGGGCAAAGAAGATTTCTAAAGAAGAATTAGATCAAACATTAACTGATCTACATAAAGAAAATTTATTATTACAAAAGT
ACTATCACTTAGATAGCATCCCAGTTGGTGATTTCTCTTTATATGACCATATATTAGATACGTCATTATTATTCAACATC
ATCCCTGAACGTTTCCAAGGAAGAACTATTGATGATGATTTATTATTTGATATTGCTCGCGGTAATAAAGACCATGTTGC
AAGTGCACTTATAAAATGGTTTAACACTAACTATCATTACATCGTACCTGAATGGGACAATGTTGAGCCTAAAGTGAGTC
GCAATGTATTATTAGATCGCTTTAAATATGCCCAATCTTTAAACGTTAACGCTCACCCTGTCATTGTTGGTCCGATTACT
TTTGTTAAATTATCAAAAGGCGGTCACCAAACTTTTGAAGAAAAAGTTAAAACGTTATTACCACTTTATAAAGAAGTGTT
TGAATCATTAATTGATGCAGGTGCTGAGTACATTCAAGTTGATGAGCCAATCTTAGTTACAGACGACAGCGAAAGCTATG
AAAATATTACACGTGAAGCTTATGACTATTTCGAAAAAGCTGGTGTTGCTAAAAAATTAGTCATTCAAACATACTTTGAA
CGTGCACATCTTAAATTCTTAAGTTCTTTACCTGTTGGTGGTTTAGGTTTAGACTTTGTCCATGATAATGGCTATAACCT
TAAACAAATTGAAGCAGGAGATTTTGATAAATCAAAAACATTATACGCTGGAATTATTGATGGTCGTAATGTATGGGCAA
GTGATATTGAAGCTAAAAAAGTCTTAATCGACAAATTGTTAGCACACACTAATGAGCTTGTCATTCAACCATCATCTTCA
TTATTACATGTTCCAGTATCTTTAGATGATGAAACATTAGATACAAGTGTTGGCGAAGGCTTAAGCTTTGCAACTGAAAA
ATTAGACGAATTAGATGCATTGCGTCGCCTATTGAATCAAAACGATAGTGTTAAATATGATAAATTAAAAGCACGTTATG
AGCGTTTCCAAAATCAATCATTCAAAAACTTAGATTATGATTTCGAAAGCGTGCGTACTTCAAGACAATCACCATTCGCG
CAACGTATTGAACAACAACAAAAACGTTTGAACTTACCGGATTTACCAACAACAACTATTGGATCATTCCCACAAAGCCG
AGAAGTTCGAAAATACCGTGCAGATTGGAAGAACAAACGCATTACAGACGAAGCATATGAAACATTCTTAAAAAATGAAA
TTGCTCGATGGATTAAAATTCAAGAAGACATCGGCTTAGATGTATTAGTTCACGGTGAATTTGAACGTAATGACATGGTT
GAATTCTTCGGAGAAAAATTACAAGGTTTCTTAGTAACTAAATTCGGTTGGGTGCAATCATATGGTTCACGCGCCGTAAA
ACCACCAATCATTTATGGTGATGTAAAATGGACAGCGCCTTTAACTGTTGATGAAACAGTTTATGCACAAAGCTTAACAG
ATAAACCAGTTAAAGGTATGTTAACTGGACCTGTAACAATTCTAAACTGGTCATTTGAACGTGTTGATTTACCACGTAAA
GTCGTTCAAGATCAAATTGCTTTAGCAATCAACGAAGAAGTATTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGT
TGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCAT
TTAAACTTGCAACGTCTTCAGTTCGTGATGAAACTCAAATCCATACACATATGTGTTATTCTCAATTCGGTCAAATCATT
CATGCTATTCATGACTTAGATGCTGATGTTATTTCAATTGAAACATCTCGTAGCCATGGTGATTTAATTAAAGACTTTGA
AGATATTAATTATGATTTAGGTATCGGTCTAGGTGTATATGATATTCATAGTCCACGTATTCCAACAAAAGAAGAAATTA
CTACAGCAATCAATCGTTCACTACAACAAATCGACCGCTCATTATTCTGGGTAAACCCTGACTGTGGTTTGAAAACGCGA
AAAGAAGAAGAAGTTAAAGATGCATTGACTGTACTTGTGAATGCTGTTAAAGCTAAACGCCAAGAATAA

Upstream 100 bases:

>100_bases
TTAATGGTTTATACATTATCACACCGTTTCAAAATGTCGAAGATTCATTAGAACTTGCAGCATACTCAAAATCTATTACT
GCTCACAAGGAGGCAATATT

Downstream 100 bases:

>100_bases
TCGCAGCATTTATCATATAATAAACTAAAAACGGGTGATTTTATGACACAATATCCTTTATGGCAACAATTAAATACTTT
AAAACAAGCAACTTGGGTTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 742; Mature: 741

Protein sequence:

>742_residues
MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNI
IPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
RAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSS
LLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLLNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFA
QRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRK
VVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQII
HAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
KEEEVKDALTVLVNAVKAKRQE

Sequences:

>Translated_742_residues
MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNI
IPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
RAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSS
LLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLLNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFA
QRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRK
VVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQII
HAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
KEEEVKDALTVLVNAVKAKRQE
>Mature_741_residues
TTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNII
PERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITF
VKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFER
AHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSL
LHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLLNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQ
RIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMVE
FFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKV
VQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIH
AIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRK
EEEVKDALTVLVNAVKAKRQE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=759, Percent_Identity=44.2687747035573, Blast_Score=600, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6320936, Length=769, Percent_Identity=42.5227568270481, Blast_Score=579, Evalue=1e-166,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_STAA1 (A7WY46)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001440943.1
- ProteinModelPortal:   A7WY46
- SMR:   A7WY46
- STRING:   A7WY46
- EnsemblBacteria:   EBSTAT00000003360
- GeneID:   5561016
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0353
- eggNOG:   COG0620
- GeneTree:   EBGT00050000023925
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   SAUR418127:SAHV_0353-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85042; Mature: 84911

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSI
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITFVKLSKGGHQTFEEKVKTLL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHH
PLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
HHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKK
HHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHH
VLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLLNQ
HHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQRIEQQQKRLNLPDLPTTTI
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
GSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCCHHHHHHHCCCCCCCCE
LTGPVTILNWSFERVDLPRKVVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRS
ECCCEEEEECCHHHHCCCHHHHHHHHHHHCCCHHEEEEECCEEEEEECCHHHHCCCCCHH
EYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
DLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
HHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
KEEEVKDALTVLVNAVKAKRQE
CHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSI
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITFVKLSKGGHQTFEEKVKTLL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHH
PLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
HHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKK
HHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHH
VLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLLNQ
HHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQRIEQQQKRLNLPDLPTTTI
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
GSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCCHHHHHHHCCCCCCCCE
LTGPVTILNWSFERVDLPRKVVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRS
ECCCEEEEECCHHHHCCCHHHHHHHHHHHCCCHHEEEEECCEEEEEECCHHHHCCCCCHH
EYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
DLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
HHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
KEEEVKDALTVLVNAVKAKRQE
CHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA