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The map label for this gene is ermA

Identifier: 15925755

GI number: 15925755

Start: 56002

End: 56733

Strand: Direct

Name: ermA

Synonym: SA0048

Alternate gene names: 15925755

Gene position: 56002-56733 (Clockwise)

Preceding gene: 15925753

Following gene: 29165619

Centisome position: 1.99

GC content: 32.51

Gene sequence:

>732_bases
ATGAACCAGAAAAACCCTAAAGACACGCAAAATTTTATTACTTCTAAAAAGCATGTAAAAGAAATATTGAATCACACGAA
TATCAGTAAACAAGACAACGTAATAGAAATCGGATCAGGAAAAGGACATTTTACCAAAGAGCTAGTCAAAATGAGTCGAT
CAGTTACTGCTATAGAAATTGATGGAGGCTTATGTCAAGTGACTAAAGAAGCGGTAAACCCCTCTGAGAATATAAAAGTG
ATTCAAACGGATATTCTAAAATTTTCCTTCCCAAAACATATAAACTATAAGATATATGGTAATATTCCTTATAACATCAG
TACGGATATTGTCAAAAGAATTACCTTTGAAAGTCAGGCTAAATATAGCTATCTTATCGTTGAGAAGGGATTTGCGAAAA
GATTGCAAAATCTGCAACGAGCTTTGGGTTTACTATTAATGGTGGAGATGGATATAAAAATGCTCAAAAAAGTACCACCA
CTATATTTTCATCCTAAGCCAAGTGTAGACTCTGTATTGATTGTTCTTGAACGACATCAACCATTGATTTCAAAGAAGGA
CTACAAAAAGTATCGATCTTTTGTTTATAAGTGGGTAAACCGTGAATATCGTGTTCTTTTCACTAAAAACCAATTCCGAC
AGGCTTTGAAGCATGCAAATGTCACTAATATTAATAAACTATCGAAGGAACAATTTCTTTCTATTTTCAATAGTTACAAA
TTGTTTCACTAA

Upstream 100 bases:

>100_bases
CAACCAAATCAAAATTAATTGGTTATAATGAACGCTTAATGTCAGTTCATTATAACCAGTAAGGAGAAGGTTATAATGAA
CCAGTAAGGAGAAGGTTATA

Downstream 100 bases:

>100_bases
ATTAAAGTAATAAAGCGTTCTCTAATTTCACAAGAGGACGCTTTATTCTTCCCAAAAATTGTTCAATATTTATCAATAAA
TCAGTAGTTTTAAAAGTAAG

Product: rRNA methylase

Products: NA

Alternate protein names: Erythromycin resistance protein; Macrolide-lincosamide-streptogramin B resistance protein

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKV
IQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYK
LFH

Sequences:

>Translated_243_residues
MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKV
IQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYK
LFH
>Mature_243_residues
MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKV
IQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYK
LFH

Specific function: This protein produces a dimethylation of the adenine residue at position 2085 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family

Homologues:

Organism=Caenorhabditis elegans, GI25146882, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ERMA_STAAM (P0A0H1)

Other databases:

- EMBL:   BA000017
- EMBL:   BA000017
- RefSeq:   NP_370576.1
- RefSeq:   NP_372179.1
- ProteinModelPortal:   P0A0H1
- SMR:   P0A0H1
- STRING:   P0A0H1
- EnsemblBacteria:   EBSTAT00000005554
- EnsemblBacteria:   EBSTAT00000006974
- GeneID:   1120012
- GeneID:   1121630
- GenomeReviews:   BA000017_GR
- GenomeReviews:   BA000017_GR
- KEGG:   sav:SAV0052
- KEGG:   sav:SAV1655
- eggNOG:   COG0030
- GeneTree:   EBGT00050000024854
- HOGENOM:   HBG535726
- OMA:   YKIFGNI
- ProtClustDB:   CLSK884354
- BioCyc:   SAUR158878:SAV0052-MONOMER
- BioCyc:   SAUR158878:SAV1655-MONOMER
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.184

Molecular weight: Translated: 28409; Mature: 28409

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 11-11 BINDING 13-13 BINDING 38-38 BINDING 59-59 BINDING 84-84 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEE
DGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQA
CCCHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCC
KYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVLERHQ
CEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCC
PLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYK
CCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
LFH
CCC
>Mature Secondary Structure
MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHFTKELVKMSRSVTAIEI
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEE
DGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQA
CCCHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCC
KYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVLERHQ
CEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEECCC
PLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYK
CCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
LFH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146