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The map label for this gene is purA

Identifier: 15925722

GI number: 15925722

Start: 22448

End: 23731

Strand: Direct

Name: purA

Synonym: SA0016

Alternate gene names: 15925722

Gene position: 22448-23731 (Clockwise)

Preceding gene: 15925721

Following gene: 15925723

Centisome position: 0.8

GC content: 36.6

Gene sequence:

>1284_bases
ATGTCATCAATCGTAGTAGTTGGGACACAATGGGGAGACGAAGGAAAAGGAAAAATAACGGATTTCTTGGCAGAACAGTC
AGATGTTATCGCGCGTTTTTCAGGTGGTAATAATGCAGGCCATACCATTCAATTTGGCGGAGAAACATATAAATTACATT
TAGTACCATCTGGTATCTTTTACAAAGACAAATTAGCGGTAATCGGTAACGGTGTCGTTGTTGATCCAGTTGCACTATTG
AAAGAATTAGACGGATTAAATGAACGTGGCATTCCTACAAGTAATTTACGTATATCTAATCGTGCGCAAGTGATTTTACC
ATATCACTTAGCACAAGATGAATATGAAGAACGTTTACGCGGTGACAATAAGATTGGTACAACTAAAAAAGGTATCGGTC
CAGCATATGTAGACAAAGTTCAACGTATCGGTATTCGTATGGCAGATTTACTTGAAAAAGAAACATTCGAAAGATTATTA
AAATCAAACATTGAATATAAACAAGCATATTTCAAAGGTATGTTTAACGAAACATGTCCATCATTTGATGATATCTTTGA
AGAATACTATGCTGCAGGTCAACGTTTAAAAGAATTTGTAACAGACACATCAAAAATCTTAGACGATGCATTTGTAGCAG
ATGAAAAGGTACTTTTCGAAGGTGCGCAAGGTGTAATGTTAGATATCGACCATGGTACATATCCATTCGTTACATCAAGT
AATCCAATTGCAGGTAACGTTACTGTTGGTACAGGTGTAGGTCCTACATTCGTTTCGAAGGTAATTGGTGTATGTAAAGC
TTATACATCACGTGTTGGTGATGGTCCATTCCCTACTGAATTATTCGATGAAGATGGACATCATATTAGAGAAGTTGGTC
GTGAATACGGTACAACAACAGGACGTCCACGTCGTGTAGGTTGGTTTGACTCAGTTGTATTACGTCACTCTCGTCGTGTA
AGTGGTATTACAGATTTATCTATTAACTCAATCGATGTTTTAACAGGCCTAGACACAGTGAAAATCTGTACAGCTTATGA
ATTAGACGGTAAAGAAATTACTGAGTACCCAGCAAACTTAGATCAATTAAAACGTTGTAAACCAATCTTTGAAGAGTTAC
CAGGTTGGACAGAAGACGTAACAAGTGTGCGTACTTTAGAAGAATTACCTGAAAATGCACGTAAATATTTAGAGCGTATT
TCAGAATTATGTAATGTACAAATTTCTATCTTCTCAGTTGGTCCAGATAGAGAACAAACAAACCTATTAAAAGAATTGTG
GTAG

Upstream 100 bases:

>100_bases
TAAAAAGGCCGAAAATCAATGTTCGATTTTTATTTGCATTATGGTCTCGATATTGGTAGAATATCAAATGGTTAAATGAG
AAAAACTTGGAGGTGCTCAC

Downstream 100 bases:

>100_bases
AACTTTATATAAGTCATACGCAATGATTATAAATACATGAGCCTTCTATCTTTATTGGTAGGAGGCTTTTGCTTTGTTTG
CTTCTGTATCGATTCGATTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALL
KELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRV
SGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPENARKYLERI
SELCNVQISIFSVGPDREQTNLLKELW

Sequences:

>Translated_427_residues
MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALL
KELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRV
SGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPENARKYLERI
SELCNVQISIFSVGPDREQTNLLKELW
>Mature_426_residues
SSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLK
ELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLLK
SNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSSN
PIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVS
GITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPENARKYLERIS
ELCNVQISIFSVGPDREQTNLLKELW

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=40.9952606635071, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI34577063, Length=431, Percent_Identity=40.1392111368909, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=39.5408163265306, Blast_Score=270, Evalue=2e-72,
Organism=Escherichia coli, GI1790620, Length=426, Percent_Identity=46.9483568075117, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=41.3145539906103, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=41.3145539906103, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=40.4109589041096, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=38.0614657210402, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47552; Mature: 47421

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIF
CCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCCE
YKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLR
EECCEEEEECCEEECHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHC
GDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLLKSNIEYKQAYFKGMFNETCP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
SFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCEEECC
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHCCCC
GRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHCCCCH
DQLKRCKPIFEELPGWTEDVTSVRTLEELPENARKYLERISELCNVQISIFSVGPDREQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHH
NLLKELW
HHHHHHC
>Mature Secondary Structure 
SSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQFGGETYKLHLVPSGIF
CCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCCE
YKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRAQVILPYHLAQDEYEERLR
EECCEEEEECCEEECHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHC
GDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLLKSNIEYKQAYFKGMFNETCP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
SFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVLFEGAQGVMLDIDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCEEECC
NPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGPFPTELFDEDGHHIREVGREYGTTT
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHCCCC
GRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVLTGLDTVKICTAYELDGKEITEYPANL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEEEECCCCHHHHCCCCH
DQLKRCKPIFEELPGWTEDVTSVRTLEELPENARKYLERISELCNVQISIFSVGPDREQT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHH
NLLKELW
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA