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The map label for this gene is nikA [H]

Identifier: 15925457

GI number: 15925457

Start: 2602649

End: 2604247

Strand: Reverse

Name: nikA [H]

Synonym: SAV2467

Alternate gene names: 15925457

Gene position: 2604247-2602649 (Counterclockwise)

Preceding gene: 15925458

Following gene: 15925456

Centisome position: 90.47

GC content: 34.46

Gene sequence:

>1599_bases
ATGAGAAAACTAACTAAAATGAGTGCAATGTTACTTGCATCAGGGCTAATTTTAACTGGTTGTGGCGGTAATAAAGGTTT
AGAGGAGAAAAAAGAAAACAAGCAATTAACGTATACGACGGTTAAAGATATCGGTGATATGAATCCGCATGTTTACGGTG
GATCAATGTCTGCTGAAAGTATGATATACGAGCCGCTTGTACGTAACACGAAAGATGGTATTAAGCCTTTACTAGCTAAA
AAATGGGATGTGTCTGAAGATGGGAAGACTTATACATTCCATTTAAGAGATGATGTGAAATTCCATGATGGAACAACATT
TGATGCTGACGCAGTTAAGAAAAATATTGATGCGGTTCAACAAAATAAAAAATTGCATTCTTGGTTAAAGATTTCAACAT
TAATTGACAATGTTAAAGTTAAAGATAAGTACACGGTTGAATTGAATTTGAAAGAAGCATATCAACCTGCATTGGCTGAA
TTAGCGATGCCTCGTCCATATGTATTTGTGTCTCCAAAAGACTTTAAAAACGGTACAACAAAAGATGGCGTTAAAAAGTT
CGATGGTACTGGTCCATTTAAATTAGGTGAACACAAAAAAGATGAGTCTGCAGACTTTAACAAAAATGATCAATACTGGG
GCGAAAAGTCTAAACTTAACAAAGTACAAGCAAAAGTAATGCCTGCTGGTGAAACAGCATTCCTATCAATGAAAAAAGGT
GAAACGAACTTTGCCTTCACAGATGATAGAGGTACGGATAGCTTAGACAAAGACTCTTTAAAACAATTGAAAGATACAGG
TGACTATCAAGTTAAGCGTAGTCAACCTATGAATACGAAAATGTTAGTTGTCAATTCTGGTAAAAAAGATAACGCTGTCA
GTGACAAAACAGTCAGACAAGCGATTGGTCATATGGTAAACAGAGATAAAATTGCCAAAGAAATTTTAGATGGTCAAGAA
AAACCAGCAACTCAATTATTTGCGAAAAATGTAACAGACATTAATTTCGATATGCCAACACGTAAGTATGACCTTAAAAA
AGCAGAATCATTATTAGATGAAGCTGGTTGGAAGAAAGGTAAAGACAGTGATGTTCGTCAAAAAGATGGTAAAAACCTTG
AAATGGCAATGTACTATGACAAAGGTTCTTCAAGTCAAAAAGAACAAGCAGAATACTTACAAGCAGAATTTAAGAAAATG
GGTATTAAGTTAAACATCAATGGCGAAACATCAGATAAAATTGCTGAACGTCGTACTTCTGGTGATTATGACTTAATGTT
CAACCAAACTTGGGGATTATTGTACGATCCACAAAGTACTATTGCAGCATTTAAAGCGAAAAATGGTTATGAAAGTGCAA
CATCAGGCATTGAGAACAAAGATAAAATATACAACAGCATTGATGACGCATTTAAAATCCAAAACGGTAAAGAGCGTTCA
GACGCTTATAAAAACATTTTGAAACAAATTGATGATGAAGGTATCTTTATCCCTATTTCACACGGTAGTATGACAGTTGT
TGCGCCGAAAGATTTAGAAAAAGTATCATTCACACAATCACAGTATGAATTACCATTCAATGAAATGCAGTATAAATAA

Upstream 100 bases:

>100_bases
TGTTTGACATTTACATAAAAATAAGCAAATAATTGAGAAAAATAATCATTACGATTTGATTAAGTAATGCAACTTATCAA
TTTAGAAAGAGGAAAAGCAA

Downstream 100 bases:

>100_bases
AGGAGCAATTAGATGTTCAAATTTATCTTAAAACGTATTGCGCTCATGTTTCCATTAGTGATTGTAGTAAGTTTTATGAC
ATTTCTATTGACGTATATTA

Product: oligopeptide transporter substrate binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAK
KWDVSEDGKTYTFHLRDDVKFHDGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQE
KPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKM
GIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

Sequences:

>Translated_532_residues
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAK
KWDVSEDGKTYTFHLRDDVKFHDGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQE
KPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKM
GIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK
>Mature_532_residues
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAK
KWDVSEDGKTYTFHLRDDVKFHDGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQE
KPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKM
GIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=499, Percent_Identity=34.0681362725451, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1789966, Length=538, Percent_Identity=27.3234200743494, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787052, Length=339, Percent_Identity=25.3687315634218, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787762, Length=458, Percent_Identity=21.6157205240175, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787551, Length=279, Percent_Identity=23.2974910394265, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60023; Mature: 60023

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAES
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEEEEHHHHCCCCCEEECCCCCHHH
MIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTTFDADAVKKNIDAVQ
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHH
QNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTT
HHHHHHHHHHHHHHHHCEEECCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCHHCCCCCC
KDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEEEECC
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQ
CCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHH
AIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
KDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTS
CCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCC
GDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
CCEEEEEECCCCEEECCHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHH
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK
HHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHCCHHCCCCHHHHCCC
>Mature Secondary Structure
MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDIGDMNPHVYGGSMSAES
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCEEEEEEHHHHCCCCCEEECCCCCHHH
MIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFHDGTTFDADAVKKNIDAVQ
HHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHH
QNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAELAMPRPYVFVSPKDFKNGTT
HHHHHHHHHHHHHHHHCEEECCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCHHCCCCCC
KDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSKLNKVQAKVMPAGETAFLSMKKG
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEEEEEECC
ETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQPMNTKMLVVNSGKKDNAVSDKTVRQ
CCCEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHH
AIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDINFDMPTRKYDLKKAESLLDEAGWKKG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCC
KDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEYLQAEFKKMGIKLNINGETSDKIAERRTS
CCCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCC
GDYDLMFNQTWGLLYDPQSTIAAFKAKNGYESATSGIENKDKIYNSIDDAFKIQNGKERS
CCEEEEEECCCCEEECCHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHH
DAYKNILKQIDDEGIFIPISHGSMTVVAPKDLEKVSFTQSQYELPFNEMQYK
HHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHCCHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]