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The map label for this gene is ycnB [H]

Identifier: 15925405

GI number: 15925405

Start: 2542720

End: 2544159

Strand: Reverse

Name: ycnB [H]

Synonym: SAV2415

Alternate gene names: 15925405

Gene position: 2544159-2542720 (Counterclockwise)

Preceding gene: 15925406

Following gene: 15925404

Centisome position: 88.38

GC content: 34.1

Gene sequence:

>1440_bases
ATGTCGAAGAAACAAAAATTAACGATGATTATTACTATGCTGATGGGTGGATTTTTTGGATTATTAAATGAAACACTATT
AGTGACGGCTTTACCAAGTATTATGAAAGATTTTGAAATTTCATATACACAAGTTCAATGGCTGACAACAGCTTTTTTAT
TGACTAATGGGATTGTTATTCCTTTGTCCGCGCTTGTTATACAACGTTATACAACAAGACAAGTGTTTTTAGTGGGTATT
TCTATCTTTTTCTTAGGTACATTACTCGGCGGCTTGAGTCCGCACTTTGCAACATTATTAGTTGCTAGAATTATTCAGGC
GTTAGGCGCAGGTATTATGATGCCATTGATGATGACAACGATTTTGGATGTTTTCCAACCGCATGAACGCGGTAAATATA
TGGGGATATTTGGTTTGGTAATTGGTTTAGCACCAGCTATTGGACCTACTCTTTCAGGTTACCTTGTTGAATATTTAAAC
TGGAGATCGCTTTTCCATGTTGTCGCTCCAATTGCAGCTGTTACATTTTTAATTGGATTTAAAACGATAAAAAATGTTGG
AACTACAATTAAAGTACCTATTGATTTTATTTCTGTCATTTTTTCTGTACTAGGTTTCGGCGGGTTATTGTATGGAACGA
GTTCAATTTCAGAAAAAGGTTTTGATAATCCTATCGTATTAGTATCTATGATTGGAGGCGTTGTTTTAGTCGCATTATTT
GTATTACGTCAATATCGGCTATCAACACCATTATTAAATTTTGCTGTATTTAAAAATAAACAATTTACAGTTGGTATCAT
TATTATGGGTGTCACAATGGTATCGATGATTGGTTCGGAAACGATTTTACCTATCTTTGTGCAAAATTTATTGCATCGTT
CAGCTTTAGATTCTGGATTAACTTTATTACCAGGAGCAATTGTTATGGCATTTATGTCGATGACTTCGGGTGCTTTATAT
GAAAAGTTTGGTCCTAGAAAGCTTGCTTTAGTAGGTATGGCGATTGTTGTTATTACTACGGCTTATTTTGTTGTAATGGA
TGAACAAACATCAACAATCATGTTGGCAACAGTTTATGCGATTCGAATGGTTGGTATCGCGTTAGGATTAATTCCAGTAA
TGACCCATACGATGAATCAATTAAAGCCAGAAATGAATGCACACGGTTCATCTATGACAAACACAGTACAACAAATTGCT
GGTTCTATCGGAACAGCTGCATTAATTACTATTTTATCTCATGCAAGCAAAAACTTTTCTCCAACTATGTCAGACTATAA
TGGCATGAACAAAATTGATATGATGAATCAAATTAAAGTTGATACGATGTTGCATGGTTATCACGCTGGATTTTTATTTG
CATTATTGATTACAGTTGTTAGTTTCTTTTGTTCATTTATGTTACAAGGGAAGAAAAAAGAAGTTGATTCTCGTCAATAA

Upstream 100 bases:

>100_bases
AAATTGTAATAAAGTCGTGCTAATTTTTTGGAAAATGGGTATAATTACCGGATATCTAAAAATGTGTGTCGTTTTTTAGA
TGGTGAGGGGGAAGCTTTAA

Downstream 100 bases:

>100_bases
ATATAAGTTGCTAGCTATATAAAGCTTTTTAGCAAAAGTTCAACATATTGACTTATCCGGCATTTCAGATTAAAATATTT
TTATTCCGATTAGAATAATA

Product: multidrug resistance protein YcnB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGI
SIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLN
WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALF
VLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALY
EKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIA
GSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Sequences:

>Translated_479_residues
MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGI
SIFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLN
WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALF
VLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALY
EKFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIA
GSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ
>Mature_478_residues
SKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGIS
IFFLGTLLGGLSPHFATLLVARIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYLNW
RSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISEKGFDNPIVLVSMIGGVVLVALFV
LRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSMIGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYE
KFGPRKLALVGMAIVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSMTNTVQQIAG
SIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAGFLFALLITVVSFFCSFMLQGKKKEVDSRQ

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=463, Percent_Identity=24.1900647948164, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788710, Length=414, Percent_Identity=24.3961352657005, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790195, Length=425, Percent_Identity=25.6470588235294, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=23.5714285714286, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788392, Length=410, Percent_Identity=22.4390243902439, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=327, Percent_Identity=26.2996941896024, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=138, Percent_Identity=24.6376811594203, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=190, Percent_Identity=23.6842105263158, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52170; Mature: 52038

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQVQWLTTAFLLTNGIVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHH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CCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]