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The map label for this gene is alsS [H]

Identifier: 15925197

GI number: 15925197

Start: 2349307

End: 2350971

Strand: Reverse

Name: alsS [H]

Synonym: SAV2207

Alternate gene names: 15925197

Gene position: 2350971-2349307 (Counterclockwise)

Preceding gene: 15925199

Following gene: 15925196

Centisome position: 81.67

GC content: 34.77

Gene sequence:

>1665_bases
ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGGGTAGAATATGTTTTTGGTAT
TCCGGGTGCAAAGATAGACTATCTATTTAATGCTTTAATTGATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAA
ATGCTGCAATGATGGCACAAGGTATTGGAAGATTAACAGGTAAACCGGGTGTAGTACTTGTTACAAGTGGCCCTGGTGTA
AGTAATTTAACGACTGGACTATTAACAGCTACATCTGAAGGGGATCCTGTATTAGCGTTAGGTGGCCAAGTGAAACGTAA
TGATTTATTACGATTAACGCATCAAAGTATTGATAATGCTGCGCTATTAAAATATTCATCAAAATACAGTGAAGAAGTAC
AAGATCCTGAATCATTATCAGAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
AGTATTCCGCAAGACGTTATTTCTTCACCAGTTGAATCTAAAGCTATATCACTTTGCCAAAAACCAAATTTAGGAGTACC
GAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAAAATGCATCATTTCCTGTTTTATTAGCTGGTATGAGAAGTT
CAAGTGCAGAAGAAACAAATGCCATTCGCAAATTAGTTGAGCGCACGAATTTACCAGTTGTAGAAACATTCCAAGGTGCA
GGTGTAATTAGTCGTGAATTAGAAAATCATTTCTTCGGTCGTGTGGGCTTATTCCGCAATCAAGTTGGTGATGAATTATT
ACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCAATTGAATACGAAGCTAGTAACTGGAATAAAGAATTAGAAA
CACAAATTATCAATATTGACGAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
GCTAAAACGATTGAAATGATTTCTGAAAAAGTGGATGAGCCATTTATAAATCAACAACATTTAGACGAATTAGAACAATT
AAGAACACATATTGATGAAGAAACTGGTATTAAAGCGACGCATGAAGAAGGAATTCTACATCCAGTGGAAATTATTGAAT
CTATGCAAAAGGTATTAACTGATGATACTACTGTAACAGTTGATGTTGGAAGTCACTATATTTGGATGGCACGTAATTTC
AGAAGTTACAATCCAAGACATTTATTATTTAGCAATGGTATGCAAACGCTTGGTGTAGCATTACCGTGGGCAATTTCAGC
TGCACTTGTGCGCCCTAATACGCAAGTTGTGTCCGTTGCTGGCGATGGTGGCTTTTTATTTTCATCACAAGATTTAGAAA
CGGCCGTACGTAAAAATTTAAATATCATCCAGCTTATTTGGAATGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
ATGAAATATAAACGTTCGTCAGGTGTAGACTTCGGTCCTGTAGATTTTGTAAAATATGCAGAATCATTTGGCGCGAAAGG
TTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATTAAAGAGGGCTATGAAACAGATGGTCCAGTATTAATTGATA
TACCTGTAAATTACAAAGATAATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA

Upstream 100 bases:

>100_bases
AACATCATTAATTTTGCGTAACTATCATTAGATTACAAATCACAATGTAATTACATGTAATACACATCTATACATCACAA
TTGGAAGGAAATGAATATAA

Downstream 100 bases:

>100_bases
AATAAAGATAAATGTTAAAGAGGAGTGGGAGATTTTATGACTAATGTTTTATACCAACATGGTACATTAGGCACATTAAT
GGCAGGATTATTAAAAGGAA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGV
SNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNI
AKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNF
RSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

Sequences:

>Translated_554_residues
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGV
SNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNI
AKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNF
RSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN
>Mature_553_residues
TDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVS
NLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFIS
IPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGAG
VISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIA
KTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFR
SYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEM
KYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=28.0141843971631, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI21361361, Length=582, Percent_Identity=22.1649484536082, Blast_Score=139, Evalue=8e-33,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=27.007299270073, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=27.1739130434783, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1787096, Length=543, Percent_Identity=26.8876611418048, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1786717, Length=579, Percent_Identity=25.3886010362694, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=24.1622574955908, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=25.4545454545455, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=25.4083484573503, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=563, Percent_Identity=24.5115452930728, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=28.719723183391, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=24.6376811594203, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6321524, Length=492, Percent_Identity=20.5284552845528, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=27.4475524475524, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61170; Mature: 61039

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQ
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNA
HHHHCCCCCCEEEEECCCCCCHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCH
ALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQ
HHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHC
KPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
CCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHEEECHH
EVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCC
HEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHH
LPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
HHHHHHHHEECCCCEEEEEECCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCEEEEEECCCCC
NIKLSTNMLPDVFN
CEEEECCCCCCCCC
>Mature Secondary Structure 
TDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQ
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNA
HHHHCCCCCCEEEEECCCCCCHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCH
ALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQ
HHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHC
KPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
CCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHEEECHH
EVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCC
HEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHH
LPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
HHHHHHHHEECCCCEEEEEECCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCEEEEEECCCCC
NIKLSTNMLPDVFN
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]