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The map label for this gene is leuS [H]

Identifier: 15924750

GI number: 15924750

Start: 1893973

End: 1896387

Strand: Reverse

Name: leuS [H]

Synonym: SAV1760

Alternate gene names: 15924750

Gene position: 1896387-1893973 (Counterclockwise)

Preceding gene: 15924751

Following gene: 15924749

Centisome position: 65.88

GC content: 34.33

Gene sequence:

>2415_bases
TTGAATTACAACCACAATCAAATTGAAAAGAAATGGCAAGACTATTGGGACGAAAATAAAACATTTAAAACAAATGATAA
CTTAGGTCAAAAGAAATTTTATGCTTTAGACATGTTTCCATATCCATCAGGTGCTGGTTTACATGTTGGACATCCTGAGG
GCTATACAGCAACAGATATCATTTCAAGATATAAAAGGATGCAAGGTTATAATGTATTACATCCGATGGGGTGGGATGCA
TTCGGATTACCAGCAGAGCAATATGCTTTAGACACTGGCAACGACCCACGTGAATTTACAAAGAAAAATATCCAAACTTT
TAAACGACAAATTAAAGAATTAGGGTTCAGTTATGATTGGGATCGTGAAGTTAATACAACAGATCCAGAATACTATAAAT
GGACACAGTGGATTTTCATACAGTTATATAACAAAGGTTTAGCATACGTTGATGAAGTTGCAGTTAACTGGTGTCCAGCA
TTAGGCACTGTTTTATCTAACGAAGAAGTGATTGATGGTGTCTCTGAACGTGGTGGACATCCAGTTTATCGTAAGCCGAT
GAAACAATGGGTACTTAAAATCACAGAATATGCAGATCAATTATTAGCAGATTTAGATGATTTAGATTGGCCTGAGTCTT
TAAAAGATATGCAGCGCAATTGGATTGGACGTTCTGAAGGGGCCAAAGTTTCATTTGATGTAGATAATACGGAAGGAAAA
GTAGAAGTATTTACGACTAGACCAGACACTATCTATGGTGCATCATTCTTAGTCTTAAGTCCTGAACATGCATTAGTTAA
TTCAATTACAACTGATGAATATAAAGAAAAAGTAAAAGCTTATCAAACAGAAGCTTCTAAAAAGTCAGATTTAGAACGTA
CAGATTTAGCAAAAGATAAATCAGGTGTGTTTACTGGTGCATATGCAATTAATCCTTTATCTGGTGAAAAAGTACAAATT
TGGATTGCTGATTATGTATTATCAACATATGGTACTGGAGCAATTATGGCAGTACCAGCGCATGATGACAGAGATTATGA
ATTTGCTAAAAAGTTTGATTTGCCAATCATTGAAGTCATCGAAGGTGGAAATGTTGAAGAAGCAGCATACACTGGTGAAG
GTAAACATATTAATTCTGGTGAACTTGATGGTTTAGAAAATGAAGCGGCAATTACTAAAGCTATTCAATTATTAGAGCAA
AAAGGTGCTGGCGAAAAGAAAGTTAATTACAAATTAAGAGATTGGTTATTCAGTCGTCAGCGTTATTGGGGCGAACCAAT
TCCTGTCATTCATTGGGAAGATGGAACAATGACAACTGTTCCTGAAGAAGAGCTACCATTGTTGTTACCTGAAACAGATG
AAATCAAGCCATCAGGGACTGGTGAGTCTCCACTAGCTAATATTGATTCATTTGTAAATGTTGTAGATGAAAAAACAGGT
ATGAAAGGACGTCGTGAAACAAATACAATGCCACAATGGGCAGGTAGTTGTTGGTATTATTTACGTTACATCGATCCTAA
AAATGAAAATATGTTAGCAGATCCTGAAAAATTAAAACATTGGTTACCTGTTGATTTATATATCGGTGGAGTAGAACATG
CGGTTCTTCACTTATTATATGCAAGATTTTGGCATAAAGTCCTTTATGATTTGGGTATCGTACCTACTAAAGAACCTTTC
CAAAAATTATTTAACCAAGGTATGATTTTAGGAGAAGGTAATGAGAAGATGAGTAAATCTAAAGGAAATGTAATCAATCC
TGATGATATAGTACAGTCTCATGGTGCAGATACTTTACGTCTTTACGAAATGTTTATGGGACCTTTAGATGCTGCAATTG
CATGGAGTGAAAAAGGATTAGATGGGTCTCGTCGATTCTTAGATCGCGTATGGCGTTTAATGGTAAATGAAGATGGAACA
TTGAGTTCAAAAATTGTAACTACAAATAATAAATCTTTAGATAAAGTTTATAACCAAACTGTTAAAAAGGTAACAGAAGA
CTTTGAAACATTAGGATTTAATACTGCTATTAGTCAATTAATGGTATTTATTAATGAGTGTTATAAAGTTGATGAAGTTT
ATAAACCTTACATTGAAGGCTTCGTTAAAATGTTAGCACCTATTGCACCACATATCGGTGAAGAATTATGGTCAAAATTA
GGACATGAAGAGTCTATTACGTACCAACCTTGGCCAACTTATGACGAAGCACTACTTGTAGATGATGAAGTAGAAATCGT
TGTTCAAGTAAATGGTAAATTGAGAGCTAAAATTAAAATTGCTAAAGATACATCAAAAGAAGAAATGCAAGAAATTGCCT
TATCTAATGACAATGTTAAAGCGAGTATTGAAGGTAAAGACATCATGAAAGTCATCGCTGTTCCTCAAAAATTAGTCAAT
ATTGTAGCTAAATAA

Upstream 100 bases:

>100_bases
TGCACAATTTTTGTGAAATAGGGTGGTAACGCGGCAAATGTCGTCCCTATGTAAATAGAATAGTTAGAGGTGTCTTTTTT
ATTGAATAGGAGGAAATGTG

Downstream 100 bases:

>100_bases
TGTTTTAAGGAGGACTTTGAAATGAAGTCAATTACTACAGATGAATTAAAAAACAAACTTTTTGAATCTAAACCAGTTCA
AATTGTTGATGTTCGTACTG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPA
LGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGK
VEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQI
WIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQ
KGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTG
MKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPF
QKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGT
LSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKL
GHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLVN
IVAK

Sequences:

>Translated_804_residues
MNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPA
LGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGK
VEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQI
WIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQ
KGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTG
MKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPF
QKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGT
LSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKL
GHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLVN
IVAK
>Mature_804_residues
MNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDA
FGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPA
LGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGK
VEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQI
WIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQ
KGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTG
MKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPF
QKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGT
LSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKL
GHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLVN
IVAK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=870, Percent_Identity=33.5632183908046, Blast_Score=458, Evalue=1e-128,
Organism=Homo sapiens, GI94721239, Length=188, Percent_Identity=29.2553191489362, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI94721241, Length=188, Percent_Identity=29.2553191489362, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI5454158, Length=192, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=3e-11,
Organism=Escherichia coli, GI1786861, Length=862, Percent_Identity=42.2273781902552, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=888, Percent_Identity=22.4099099099099, Blast_Score=140, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71997510, Length=278, Percent_Identity=39.568345323741, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI71997517, Length=274, Percent_Identity=39.7810218978102, Blast_Score=196, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI212645227, Length=345, Percent_Identity=29.5652173913043, Blast_Score=149, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17510661, Length=455, Percent_Identity=23.956043956044, Blast_Score=90, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17554638, Length=298, Percent_Identity=26.1744966442953, Blast_Score=76, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323414, Length=813, Percent_Identity=37.1463714637146, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321531, Length=385, Percent_Identity=25.4545454545455, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319395, Length=180, Percent_Identity=30.5555555555556, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355409, Length=862, Percent_Identity=32.2505800464037, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28574730, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24668547, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24668543, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 91672; Mature: 91672

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHHH
ISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGGH
CCCCCCCCCCHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHCCCC
PVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
VEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDK
EEEEEECCCCEECCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
SGVFTGAYAINPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVI
CCCEEEEEEECCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEEE
EGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSRQ
CCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTG
HHCCCCCCEEEECCCCEECCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
MKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHH
ARFWHKVLYDLGIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHHHCCCCHHH
LYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQT
HHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHH
VKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKL
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVK
CCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHCCCCEE
ASIEGKDIMKVIAVPQKLVNIVAK
EEECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCEECCCCCCCCHHHH
ISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
DREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGGH
CCCCCCCCCCHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHCHHHCCCC
PVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCE
VEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDK
EEEEEECCCCEECCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
SGVFTGAYAINPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVI
CCCEEEEEEECCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEEE
EGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSRQ
CCCCCCHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
RYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTG
HHCCCCCCEEEECCCCEECCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
MKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLY
CCCCCCCCCCCHHCCCEEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHH
ARFWHKVLYDLGIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCHHHHHCCCCCCCHHHHHHHCCCCHHH
LYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQT
HHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHH
VKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKL
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVK
CCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHCCCCEE
ASIEGKDIMKVIAVPQKLVNIVAK
EEECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12044378 [H]