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The map label for this gene is iscS2 [H]

Identifier: 15924706

GI number: 15924706

Start: 1834783

End: 1835922

Strand: Reverse

Name: iscS2 [H]

Synonym: SAV1716

Alternate gene names: 15924706

Gene position: 1835922-1834783 (Counterclockwise)

Preceding gene: 15924707

Following gene: 15924705

Centisome position: 63.78

GC content: 31.75

Gene sequence:

>1140_bases
TTGATATATCTAGATAATGCGGCAACGACGAAAGCATTTGAAGAAGTGTTAGATACTTATTTAAAAGTAAATCAATCAAT
GTATTATAATCCGAATAGTCCGCATAAAGCTGGTTTGCAGGCAAATCAATTACTACAACAAGCAAAAGCCCAAATTAATG
CAATGATTAATTCAAAAACAAATTATGATGTTGTATTCACTAGTGGTGCAACTGAATCCAATAATCTTGCTTTAAAAGGT
ATTGCCTATCGTAAATTTGATACAGCGAAGGAAATAATTACATCCGTGTTAGAGCATCCGTCCGTATTAGAGGTTGTAAG
ATATTTGGAAGCACACGAAGGATTTAAAGTTAAATATGTTGATGTAAAGAAAGATGGCAGTATTAACTTAGAACACTTCA
AAGAATTAGTGTCAGACAAAGTCGGTTTAGTAACATGTATGTATGTAAATAATGTAACTGGACAAATACAGCCTATTCCA
CAAATGGCTAAAGTTATAAAAAATTATCCTAAGGCACATTTTCATGTAGATGCGGTTCAAGCATTCGGCAAAATTTCAAT
GGATCTCAATAACATAGATAGTATTAGTTTAAGTGGACACAAGTTTAATGGTTTAAAAGGACAAGGCGTCTTACTTGTAA
ATCACATTCAAAATGTTGAACCAACTGTCCATGGTGGTGGTCAAGAATATGGCGTTAGAAGTGGAACAGTTAATTTGCCA
AATGATATTGCAATGGTTAAAGCGATGAAGATAGCTAATGAAAACTTTGAAGCATTGAATGCATTTGTTACTGAGTTAAA
TAATGACGTCCGTCAATTTTTAAATAAATATCATGGAGTTTATATTAATTCTTCAACTTCAGGTTCACCATTCGTTTTAA
ATATTAGTTTTCCTGGCGTAAAAGGTGAAGTATTAGTTAATGCTTTTTCAAAATATGACATTATGATATCTACGACGAGT
GCTTGTTCATCTAAACGTAATAAATTAAATGAAGTATTGGCTGCAATGGGATTATCAGACAAATCTATTGAAGGTAGTAT
AAGATTATCATTTGGGGCTACTACAACTAAAGAAGATATAGCAAGGTTTAAAGAAATATTTATCATCATTTATGAGGAAA
TTAAGGAGTTGCTAAAATAA

Upstream 100 bases:

>100_bases
TTATTTTGCGCTATAACCTTTATTTTTTGTCGGATAATAGTATAATGCTTGAGTAAAACAATTTTAACGATTGATGATTA
ATGAAAGAGAAGTGTTTATT

Downstream 100 bases:

>100_bases
TGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGAAAAAAATTTGTAAATCAATTA
AGAAATAATGTAAATAAGTC

Product: iron-sulfur cofactor synthesis protein nifZ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKG
IAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIP
QMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLP
NDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTS
ACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

Sequences:

>Translated_379_residues
MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKG
IAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIP
QMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLP
NDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTS
ACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK
>Mature_379_residues
MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKTNYDVVFTSGATESNNLALKG
IAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIP
QMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLP
NDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTS
ACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=383, Percent_Identity=35.509138381201, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI156713448, Length=400, Percent_Identity=29, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI48994898, Length=367, Percent_Identity=37.6021798365123, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789175, Length=385, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787970, Length=231, Percent_Identity=26.4069264069264, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=37.0466321243523, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17533177, Length=315, Percent_Identity=29.8412698412698, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319831, Length=365, Percent_Identity=38.6301369863014, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=36.1979166666667, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41931; Mature: 41931

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKT
CEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
NYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYV
CEEEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
DVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQ
EECCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEECCHHHHHHHHHCCCCEEEHHHHH
AFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLP
HHHHHEECCCCCCEEEECCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
NDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCC
KGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDI
CCHHHEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHH
ARFKEIFIIIYEEIKELLK
HHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQANQLLQQAKAQINAMINSKT
CEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
NYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEHPSVLEVVRYLEAHEGFKVKYV
CEEEEEECCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEE
DVKKDGSINLEHFKELVSDKVGLVTCMYVNNVTGQIQPIPQMAKVIKNYPKAHFHVDAVQ
EECCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEECCHHHHHHHHHCCCCEEEHHHHH
AFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNHIQNVEPTVHGGGQEYGVRSGTVNLP
HHHHHEECCCCCCEEEECCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
NDIAMVKAMKIANENFEALNAFVTELNNDVRQFLNKYHGVYINSSTSGSPFVLNISFPGV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEECCCC
KGEVLVNAFSKYDIMISTTSACSSKRNKLNEVLAAMGLSDKSIEGSIRLSFGATTTKEDI
CCHHHEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHH
ARFKEIFIIIYEEIKELLK
HHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]