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The map label for this gene is holB [H]

Identifier: 15923474

GI number: 15923474

Start: 538933

End: 539859

Strand: Direct

Name: holB [H]

Synonym: SAV0484

Alternate gene names: 15923474

Gene position: 538933-539859 (Clockwise)

Preceding gene: 15923473

Following gene: 15923475

Centisome position: 18.72

GC content: 30.74

Gene sequence:

>927_bases
ATGGATGAACAGCAACAATTGACGAATGCATATCATTCAAATAAATTATCGCATGCCTATTTATTTGAAGGTGATGATGC
ACAAACGATGAAACAAGTTGCGATTAATTTTGCAAAGCTTATTTTATGTCAAACAGATAGTCAATGTGAAACAAAGGTTA
GTACATATAATCATCCAGACTTTATGTATATATCAACAACTGAGAATGCAATTAAGAAAGAACAAGTTGAACAACTTGTG
CGTCATATGAATCAACTTCCTATAGAAAGCACAAATAAAGTGTACATCATTGAAGACTTTGAAAAGTTAACTGTTCAAGG
GGAAAACAGTATCTTGAAATTTCTTGAAGAACCACCGGACAATACGATTGCTATTTTATTGTCTACAAAACCTGAGCAAA
TTTTAGACACAATCCATTCAAGGTGTCAGCATGTGTATTTCAAGCCTATTGATAAAGAAAAGTTTATAAATAGATTAGTT
GAACAAAACATGTCTAAGCCAGTAGCTGAAATGATTAGTACTTATACTACGCAAATAGATAATGCAATAGCTTTAAATGA
AGAATTTGATTTATTAGCATTAAGGAAATCAGTTATACGTTGGTGTGAATTGTTGCTTACTAACAAGCCAATGGCACTTA
TAGGTATTATTGATTTATTGAAACAGGCTAAAAATAAAAAACTGCAATCTTTAACTATTGCAGCTGTGAATGGTTTCTTC
GAAGATATCATACATACAAAGGTAAATGTAGAGGATAAACAAATATATAGTGATTTAAAAAATGATATTGATCAATATGC
GCAAAAGTTGTCGTTTAATCAATTAATTTTGATGTTTGATCAACTGACGGAAGCACATAAGAAATTGAATCAAAATGTAA
ATCCAACGCTTGTATTTGAACAAATCGTAATTAAGGGTGTGAGTTAG

Upstream 100 bases:

>100_bases
TGGTTCTAAAAATAATCACAAATCATGGTCGCTATTGTTGCAGTAATTAGTTGCTCTTTGGCAACCTTTTTATATAAAAG
CAAAAGGGAGTTTGTAATGA

Downstream 100 bases:

>100_bases
ATGCCAAATGTAATAGGTGTTCAGTTTCAAAAAGCGGGAAAATTAGAATATTATACACCTAATGATATACAAGTAGATAT
AGAAGACTGGGTAGTTGTCG

Product: DNA polymerase III delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLV
RHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
EQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFF
EDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS

Sequences:

>Translated_308_residues
MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLV
RHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
EQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFF
EDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS
>Mature_308_residues
MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLV
RHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
EQNMSKPVAEMISTYTTQIDNAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFF
EDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=161, Percent_Identity=27.9503105590062, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35498; Mature: 35498

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPD
CCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCC
FMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPD
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHEECCCHHHHHHHHCCCC
NTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLVEQNMSKPVAEMISTYTTQID
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
NAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFF
CEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QIVIKGVS
HHHHHCCC
>Mature Secondary Structure
MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQTDSQCETKVSTYNHPD
CCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCC
FMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEKLTVQGENSILKFLEEPPD
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHEECCCHHHHHHHHCCCC
NTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLVEQNMSKPVAEMISTYTTQID
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
NAIALNEEFDLLALRKSVIRWCELLLTNKPMALIGIIDLLKQAKNKKLQSLTIAAVNGFF
CEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLILMFDQLTEAHKKLNQNVNPTLVFE
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QIVIKGVS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]