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The map label for this gene is tagF [H]

Identifier: 15923244

GI number: 15923244

Start: 296756

End: 297925

Strand: Direct

Name: tagF [H]

Synonym: SAV0254

Alternate gene names: 15923244

Gene position: 296756-297925 (Clockwise)

Preceding gene: 15923243

Following gene: 15923245

Centisome position: 10.31

GC content: 33.42

Gene sequence:

>1170_bases
ATGATTAAAAATACAATTAAAAAATTGATAGAACATAGTATATATACGACTTTTAAATTACTATCAAAATTGCCAAACAA
GAATCTAATTTATTTTGAAAGCTTTCATGGTAAACAATACAGCGACAACCCCAAAGCATTATATGAATACTTAACTGAAC
ATAGCGATGCCCAATTAATATGGGGTGTGAAAAAAGGATATGAACACATATTCCAACAGTACAATGTACCATATGTTACA
AAGTTTTCAATGAAATGGTTTTTAGCGATGCCAAGAGCGAAAGCGTGGATGATTAACACACGTACACCAGATTGGTTATA
TAAATCACCGCGAACGACGTACTTACAAACATGGCATGGCACGCCATTAAAAAAGATTGGTTTGGATATTAGTAACGTTA
AAATGCTAGGAACAAATACTCAAAATTACCAAGATGGCTTTAAAAAAGAAAGCCAACGGTGGGATTATCTAGTGTCACCT
AATCCATATTCGACATCGATATTTCAACATGCATTTCATGTTAGTCGAGATAAGATTTTGGAAACAGGTTATCCAAGAAA
TGATAAATTATCACATAAACGCAATGATACTGAATATATTAATGGTATTAAGACAAGATTAAATATTCCATTAGATAAAA
AAGTGATTATGTACGCGCCAACTTGGCGTGACGATGAAGCGATTCGAGAAGGTTCATATCAATTTAATGTTAACTTTGAT
ATAGAAGCTTTGCGTCAAGCGCTGGATGATGATTATGTTATTTTATTACGCATGCATTATTTAGTTGTGACACGTATTGA
TGAACATGATGATTTTGTGAAAGACGTTTCAGATTATGAAGACATTTCGGATTTATACTTAATCAGCGATGCGTTAGTTA
CCGACTACTCATCTGTCATGTTCGACTTCGGTGTATTAAAGCGTCCGCAAATTTTCTATGCATATGACTTAGATAAATAT
GGCGATGAGCTTAGAGGTTTTTACATGGATTATAAAAAAGAGTTGCCAGGTCCAATTGTTGAAAATCAAACAGCACTCAT
TGATGCATTAAAACAAATCGATGAGACTGCAAATGAGTATATTGAAGCACGAACGGTATTTTATCAAAAATTCTGTTCAT
TAGAAGATGGACAAGCGTCACAACGAATTTGCCAAACGATTTTTAAGTGA

Upstream 100 bases:

>100_bases
GGTTAAATGTATTTTATGATGAAATGCTATAATAGGCATGGTTACAATGAGCTTGCTCATACATATTAATATAATTACAA
AAACACGTCGGAGGTACGAC

Downstream 100 bases:

>100_bases
TAACTTAAAAACAATAAAAAATTATAAATTAATTAGTTAAGTGATATAAATAATAAACGAAATGTTTGCTTGTATGTTAT
TATTTGTGTATGAAAACGTC

Product: trancated TagF

Products: NA

Alternate protein names: CGPTase; Major teichoic acid biosynthesis protein F; Polyglycerol phosphate polymerase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNPKALYEYLTEHSDAQLIWGVKKGYEHIFQQYNVPYVT
KFSMKWFLAMPRAKAWMINTRTPDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMYAPTWRDDEAIREGSYQFNVNFD
IEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDVSDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKY
GDELRGFYMDYKKELPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK

Sequences:

>Translated_389_residues
MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNPKALYEYLTEHSDAQLIWGVKKGYEHIFQQYNVPYVT
KFSMKWFLAMPRAKAWMINTRTPDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMYAPTWRDDEAIREGSYQFNVNFD
IEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDVSDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKY
GDELRGFYMDYKKELPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK
>Mature_389_residues
MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNPKALYEYLTEHSDAQLIWGVKKGYEHIFQQYNVPYVT
KFSMKWFLAMPRAKAWMINTRTPDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMYAPTWRDDEAIREGSYQFNVNFD
IEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDVSDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKY
GDELRGFYMDYKKELPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK

Specific function: Is responsible for the polymerization of the main chain of the major teichoic acid by sequential transfer of glycerol phosphate units from CDP-glycerol to the dissacharide linkage unit. Synthesizes polymers of approximately 35 glycerol phosphate units in

COG id: COG1887

COG function: function code M; Putative glycosyl/glycerophosphate transferases involved in teichoic acid biosynthesis TagF/TagB/EpsJ/RodC

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CDP-glycerol glycerophosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007554 [H]

Pfam domain/function: PF04464 Glyphos_transf [H]

EC number: =2.7.8.12 [H]

Molecular weight: Translated: 46011; Mature: 46011

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNPKALYEYLTEHSDAQLI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEE
WGVKKGYEHIFQQYNVPYVTKFSMKWFLAMPRAKAWMINTRTPDWLYKSPRTTYLQTWHG
HHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCEEEEECCCCCHHCCCCCCCEEEECCC
TPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSPNPYSTSIFQHAFHVSRDKIL
CCHHHHCCCHHCEEEECCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHH
ETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMYAPTWRDDEAIREGSYQFNVNFD
HCCCCCCCCCCCCCCCHHHHCCHHHEECCCCCCEEEEECCCCCCHHHHHCCCEEEEECCC
IEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDVSDYEDISDLYLISDALVTDYSSVM
HHHHHHHCCCCCEEHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKELPGPIVENQTALIDALKQIDETANEY
HHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IEARTVFYQKFCSLEDGQASQRICQTIFK
HHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNPKALYEYLTEHSDAQLI
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEE
WGVKKGYEHIFQQYNVPYVTKFSMKWFLAMPRAKAWMINTRTPDWLYKSPRTTYLQTWHG
HHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCEEEEECCCCCHHCCCCCCCEEEECCC
TPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSPNPYSTSIFQHAFHVSRDKIL
CCHHHHCCCHHCEEEECCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHH
ETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMYAPTWRDDEAIREGSYQFNVNFD
HCCCCCCCCCCCCCCCHHHHCCHHHEECCCCCCEEEEECCCCCCHHHHHCCCEEEEECCC
IEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDVSDYEDISDLYLISDALVTDYSSVM
HHHHHHHCCCCCEEHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKELPGPIVENQTALIDALKQIDETANEY
HHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IEARTVFYQKFCSLEDGQASQRICQTIFK
HHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2507871; 9384377; 1309530; 12637499 [H]