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The map label for this gene is rsmB [H]

Identifier: 15921679

GI number: 15921679

Start: 1394933

End: 1395982

Strand: Direct

Name: rsmB [H]

Synonym: ST1390

Alternate gene names: 15921679

Gene position: 1394933-1395982 (Clockwise)

Preceding gene: 15921678

Following gene: 15921680

Centisome position: 51.76

GC content: 30.38

Gene sequence:

>1050_bases
ATGGTTGAAAGATTAAATTATGATGAATTTGTGATGGAAGATTTAAAGAACGTTTATAAAGGATTTATTAATGATTTTTT
GAGATCTATTTTAGTTCCTAATAGTAGGCTTTATGTTAGAGTAAATACTTTGAAGATTAATGTAGAGGAAATCTTAGCTG
AATTAAATTTTCTTGAAAGGGATGAAGATTTTGAAGAAGCTCTTTTTGTGAAGCTAAAGGGACCAAATAAAATTGAAGAG
CATGAGACAAAGGTAATTGTAGATAAACGTACTGCTGAAAGTGTGATTATGGGTGCTGATGTATATCGTCCAGGAATAAA
GAAAGTACTTGGAAATGGTAAGTATGTTAACGTTGTTAGTGAGAACGGTGTAATAGTAGGTGAAGGGGAATTAGTAAATA
AAGGCAATTTAGTTGTTAGAGTTTTAAACCCATTGTATTCAGCTCCTAAGTTTGCTGATTTAGAGTATGTTAAAGATGGC
TCACTAATCTTACAAGGAAAGGCTTCAATGTATGTTGCTCACTTGCTTGACCCAAAACCTAATGAAAAAATTATTGATAT
GACGGCTTATCCTGGAGGGAAATTAACTCATATATATCAACTTGAACCTAGGAGTAAGGTTATTGGTTTTGATCATACCA
AGAAAAAAGTAGATGAGTTAAGAGAAAAAGTAAAAAAGATGAAAATGAACATAGAAGTTTATTTGGCAGATTCTAGGTAT
CTTTATGAGGGTTTTGGATTAAGGGATGTAGATAAAGTAATTATTGATCCACCTTGTTCAGCTCTAGGTATAAGACCAAA
GGTTTATGACAGGAAAACTAAAGAAGATATTATTAATTTTCATAATTATCAGAGACAATTTATTAATTCTGCATATAAGA
TTCTGAAGAAAAATGGCATATTAGTTTATTCTACTTGTACAGTAACTACATGGGAAAATGAGAAGGTAATTGATGATGAA
AGATTTGAAGTTGAAGATATTATTAGATTTCATCCCAATATTCATGATATGACAGGATTTTTTATAGCCAAATTAATTAA
GAGAAAATGA

Upstream 100 bases:

>100_bases
CAGTATATACTTTTGTAAGAAGCAGAAGGGAAGGAGAAGAGTTAGTTTCCTATTTTGGTGGTTTTGTTACAGAGCCTAAT
AATGAGGGTGCAAAAGTCTT

Downstream 100 bases:

>100_bases
TGGTAATAGAAGAATATTTAGGTAAGAAACCTAAAATTGCTGACAAAGTGTATATTCATCCTACAGCTTATGTAATAGGA
GATGTAAGTATAGGAGAGTT

Product: Sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MVERLNYDEFVMEDLKNVYKGFINDFLRSILVPNSRLYVRVNTLKINVEEILAELNFLERDEDFEEALFVKLKGPNKIEE
HETKVIVDKRTAESVIMGADVYRPGIKKVLGNGKYVNVVSENGVIVGEGELVNKGNLVVRVLNPLYSAPKFADLEYVKDG
SLILQGKASMYVAHLLDPKPNEKIIDMTAYPGGKLTHIYQLEPRSKVIGFDHTKKKVDELREKVKKMKMNIEVYLADSRY
LYEGFGLRDVDKVIIDPPCSALGIRPKVYDRKTKEDIINFHNYQRQFINSAYKILKKNGILVYSTCTVTTWENEKVIDDE
RFEVEDIIRFHPNIHDMTGFFIAKLIKRK

Sequences:

>Translated_349_residues
MVERLNYDEFVMEDLKNVYKGFINDFLRSILVPNSRLYVRVNTLKINVEEILAELNFLERDEDFEEALFVKLKGPNKIEE
HETKVIVDKRTAESVIMGADVYRPGIKKVLGNGKYVNVVSENGVIVGEGELVNKGNLVVRVLNPLYSAPKFADLEYVKDG
SLILQGKASMYVAHLLDPKPNEKIIDMTAYPGGKLTHIYQLEPRSKVIGFDHTKKKVDELREKVKKMKMNIEVYLADSRY
LYEGFGLRDVDKVIIDPPCSALGIRPKVYDRKTKEDIINFHNYQRQFINSAYKILKKNGILVYSTCTVTTWENEKVIDDE
RFEVEDIIRFHPNIHDMTGFFIAKLIKRK
>Mature_349_residues
MVERLNYDEFVMEDLKNVYKGFINDFLRSILVPNSRLYVRVNTLKINVEEILAELNFLERDEDFEEALFVKLKGPNKIEE
HETKVIVDKRTAESVIMGADVYRPGIKKVLGNGKYVNVVSENGVIVGEGELVNKGNLVVRVLNPLYSAPKFADLEYVKDG
SLILQGKASMYVAHLLDPKPNEKIIDMTAYPGGKLTHIYQLEPRSKVIGFDHTKKKVDELREKVKKMKMNIEVYLADSRY
LYEGFGLRDVDKVIIDPPCSALGIRPKVYDRKTKEDIINFHNYQRQFINSAYKILKKNGILVYSTCTVTTWENEKVIDDE
RFEVEDIIRFHPNIHDMTGFFIAKLIKRK

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI32698918, Length=356, Percent_Identity=28.6516853932584, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI76150625, Length=238, Percent_Identity=30.2521008403361, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI76150623, Length=238, Percent_Identity=30.2521008403361, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI2367212, Length=220, Percent_Identity=28.6363636363636, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87081985, Length=231, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536757, Length=235, Percent_Identity=28.5106382978723, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71998419, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115534021, Length=230, Percent_Identity=28.695652173913, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324268, Length=237, Percent_Identity=30.379746835443, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324306, Length=216, Percent_Identity=26.8518518518519, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319447, Length=190, Percent_Identity=27.3684210526316, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356513, Length=339, Percent_Identity=26.5486725663717, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24668781, Length=339, Percent_Identity=26.5486725663717, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI22024126, Length=265, Percent_Identity=29.4339622641509, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 40344; Mature: 40344

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVERLNYDEFVMEDLKNVYKGFINDFLRSILVPNSRLYVRVNTLKINVEEILAELNFLER
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEHHHHHHHHHHHCC
DEDFEEALFVKLKGPNKIEEHETKVIVDKRTAESVIMGADVYRPGIKKVLGNGKYVNVVS
CCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHEEECCHHHCCHHHHHHCCCCEEEEEE
ENGVIVGEGELVNKGNLVVRVLNPLYSAPKFADLEYVKDGSLILQGKASMYVAHLLDPKP
CCCEEEECCCEECCCCEEEEEEHHHHCCCCCCCCEEECCCCEEEECCHHEEEEEECCCCC
NEKIIDMTAYPGGKLTHIYQLEPRSKVIGFDHTKKKVDELREKVKKMKMNIEVYLADSRY
CCEEEEEEECCCCCEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCEE
LYEGFGLRDVDKVIIDPPCSALGIRPKVYDRKTKEDIINFHNYQRQFINSAYKILKKNGI
EEECCCCCCCCCEEECCCCHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
LVYSTCTVTTWENEKVIDDERFEVEDIIRFHPNIHDMTGFFIAKLIKRK
EEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVERLNYDEFVMEDLKNVYKGFINDFLRSILVPNSRLYVRVNTLKINVEEILAELNFLER
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEHHHHHHHHHHHCC
DEDFEEALFVKLKGPNKIEEHETKVIVDKRTAESVIMGADVYRPGIKKVLGNGKYVNVVS
CCCCCEEEEEEECCCCCCCCCCCEEEEECCCHHHEEECCHHHCCHHHHHHCCCCEEEEEE
ENGVIVGEGELVNKGNLVVRVLNPLYSAPKFADLEYVKDGSLILQGKASMYVAHLLDPKP
CCCEEEECCCEECCCCEEEEEEHHHHCCCCCCCCEEECCCCEEEECCHHEEEEEECCCCC
NEKIIDMTAYPGGKLTHIYQLEPRSKVIGFDHTKKKVDELREKVKKMKMNIEVYLADSRY
CCEEEEEEECCCCCEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCEE
LYEGFGLRDVDKVIIDPPCSALGIRPKVYDRKTKEDIINFHNYQRQFINSAYKILKKNGI
EEECCCCCCCCCEEECCCCHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
LVYSTCTVTTWENEKVIDDERFEVEDIIRFHPNIHDMTGFFIAKLIKRK
EEEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]