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The map label for this gene is folE

Identifier: 15921673

GI number: 15921673

Start: 1389143

End: 1389790

Strand: Direct

Name: folE

Synonym: ST1385

Alternate gene names: 15921673

Gene position: 1389143-1389790 (Clockwise)

Preceding gene: 15921672

Following gene: 15921674

Centisome position: 51.55

GC content: 33.64

Gene sequence:

>648_bases
ATGGGGGTCAATAACATTATTGTTATGGAAGAGACATTAAACCAAGAAAAGATGGTTGAAGAGATAGCTAAAAGGATAAG
AGAGATCCTTGAAATTTTAGGGGAAAACCCGGATAGGGAAGGACTAAGAGAAACTCCATTAAGGGTAGCTAGAGCTTTAT
TAGAAATGACAAGTGGACTTAGAACTCCTCAACCTCAAATTAAAACATTTAACCTATCCGAAGATGGTATATCTGAAGTT
GAAGACCAAATTATTCTTGTAAAGAATATAGGTTTTTCTTCTTTATGTGAACATCATTTACTACCTATAATAGGAAAAGT
ACATGTAGCATATATAGTAGGTCAAGAAAGAAAAGTAGCCGGTTTTAGTAAGATTATAAGGATTGTGAATTATTACGCAT
CAAGGCCACAAATTCAAGAAAGATTAGTTCAGCAAATTGCTGATGCTATAATGAATAGCGATATTCATCCAAAAGGTGTA
ATGGTTATAGGAGATGCTCTTCATATGTGTGCATACGTTAGGGGTGTTAAAGATAGAGAGGCAAGTTTATTATCAGTAGC
AACGAGAGGGTTATTCAAAAATAATGTGTCATTAAGGAACTATGTATTTAGATTACTCGAAACGTCAAAGAAGACAAGTT
TATTATAG

Upstream 100 bases:

>100_bases
AGATACAGTTATTGTAACAAAGAGAAAACCCATTGTTCTTGAGACTACATTCAAGTATTTATAACTTTTTAGTACTCTTT
TTAATTTAACTTTATATTTA

Downstream 100 bases:

>100_bases
GTAAAATAGATTTTTTATTGAATGAGTGACTCAAAATCTAGGATTATTGAAATCTTAAAAAAGTTTGGTGAATTACCTCA
ATCTGAACTAGTTAGGATTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MGVNNIIVMEETLNQEKMVEEIAKRIREILEILGENPDREGLRETPLRVARALLEMTSGLRTPQPQIKTFNLSEDGISEV
EDQIILVKNIGFSSLCEHHLLPIIGKVHVAYIVGQERKVAGFSKIIRIVNYYASRPQIQERLVQQIADAIMNSDIHPKGV
MVIGDALHMCAYVRGVKDREASLLSVATRGLFKNNVSLRNYVFRLLETSKKTSLL

Sequences:

>Translated_215_residues
MGVNNIIVMEETLNQEKMVEEIAKRIREILEILGENPDREGLRETPLRVARALLEMTSGLRTPQPQIKTFNLSEDGISEV
EDQIILVKNIGFSSLCEHHLLPIIGKVHVAYIVGQERKVAGFSKIIRIVNYYASRPQIQERLVQQIADAIMNSDIHPKGV
MVIGDALHMCAYVRGVKDREASLLSVATRGLFKNNVSLRNYVFRLLETSKKTSLL
>Mature_214_residues
GVNNIIVMEETLNQEKMVEEIAKRIREILEILGENPDREGLRETPLRVARALLEMTSGLRTPQPQIKTFNLSEDGISEVE
DQIILVKNIGFSSLCEHHLLPIIGKVHVAYIVGQERKVAGFSKIIRIVNYYASRPQIQERLVQQIADAIMNSDIHPKGVM
VIGDALHMCAYVRGVKDREASLLSVATRGLFKNNVSLRNYVFRLLETSKKTSLL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=188, Percent_Identity=38.2978723404255, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI4503949, Length=188, Percent_Identity=38.2978723404255, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI66932970, Length=164, Percent_Identity=39.6341463414634, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI66932972, Length=146, Percent_Identity=42.4657534246575, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1788476, Length=185, Percent_Identity=37.2972972972973, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560486, Length=195, Percent_Identity=36.4102564102564, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=41.4364640883978, Blast_Score=126, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24656782, Length=194, Percent_Identity=35.5670103092784, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24656796, Length=194, Percent_Identity=35.5670103092784, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24656791, Length=194, Percent_Identity=35.5670103092784, Blast_Score=117, Evalue=5e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_SULTO (Q971G9)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377342.1
- ProteinModelPortal:   Q971G9
- SMR:   Q971G9
- GeneID:   1459413
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1385
- NMPDR:   fig|273063.1.peg.1510
- HOGENOM:   HBG370191
- OMA:   FSSHCEH
- ProtClustDB:   PRK09347
- BioCyc:   STOK273063:ST1385-MONOMER
- BRENDA:   3.5.4.16
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24276; Mature: 24145

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVNNIIVMEETLNQEKMVEEIAKRIREILEILGENPDREGLRETPLRVARALLEMTSGL
CCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
RTPQPQIKTFNLSEDGISEVEDQIILVKNIGFSSLCEHHLLPIIGKVHVAYIVGQERKVA
CCCCCCCEEECCCCCCHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCHHH
GFSKIIRIVNYYASRPQIQERLVQQIADAIMNSDIHPKGVMVIGDALHMCAYVRGVKDRE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCHHH
ASLLSVATRGLFKNNVSLRNYVFRLLETSKKTSLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GVNNIIVMEETLNQEKMVEEIAKRIREILEILGENPDREGLRETPLRVARALLEMTSGL
CCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
RTPQPQIKTFNLSEDGISEVEDQIILVKNIGFSSLCEHHLLPIIGKVHVAYIVGQERKVA
CCCCCCCEEECCCCCCHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHEECCCCHHH
GFSKIIRIVNYYASRPQIQERLVQQIADAIMNSDIHPKGVMVIGDALHMCAYVRGVKDRE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCHHH
ASLLSVATRGLFKNNVSLRNYVFRLLETSKKTSLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479