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The map label for this gene is glyA

Identifier: 15921638

GI number: 15921638

Start: 1361121

End: 1362422

Strand: Direct

Name: glyA

Synonym: ST1354

Alternate gene names: 15921638

Gene position: 1361121-1362422 (Clockwise)

Preceding gene: 15921637

Following gene: 15921645

Centisome position: 50.51

GC content: 34.33

Gene sequence:

>1302_bases
ATGAGTCAAATTCCTAAAGAGCTTGAAAAAGTAATTGAGCTAACAAGAGAACAAAATAGATGGAGAAGAACAGAGGTAAT
AAATTTAATAGCTTCTGAAAACGTAATGAGCCCTTTAGCTGAAACGGTTTATATGAGTGACTTTATGTCAAGATATGCAG
AAGGTAAGCCATATAAAAGATATTATCAAGGAACAAAGTATGTTGATGAAGTTGAAACATTAGCTATGCAATTAATGAAT
GAAATTACTAATACTAAATTCTGTGATTTACGAGCTACTAGTGGGACTATAGCAAATGCTGCTGTGTTTAGAGTACTAGC
TAACCCTGGTGAAAAGGCATTAATTGCTCCAGTTCAAGCTGGGGCCCATGTAAGTCATACTAAATTTGGCACATTGGGAG
CTTTAGGCATAGAACATATTGAACTTCCTTATGATGCTGATAAAATGAATGTAGATGTAGATAAAGCCATAAAGATGATT
GAACAGATTAAGCCTAAATTTATTGTAATGGGAGGAAGTTTATATTTATTTCCTCATCCAGTTAAAGAATTAGCCCCTCA
TGCACATGCTGTTGGAGCTAAAGTAGTCTATGATGCTGCTCATGTTTATGGTTTAATAACTGGAAAAGCGTGGCATAATC
CATTAGAAGAAGGAGCAGATATTATGACTTCTTCAACGCATAAAACTTTCCCTGGACCTCAAGGTGGTGCTGTATTTTCA
AATGAAGAAGAAATATTTAAGAAAGTTGCTGACACAATATTTCCTTGGTTTGTTAGTAATCATCATTTACATAGGTTACC
AGCCACTGCTGTTACTGCTCTTGAAATGAAGTATTTTGGTGAAGATTATGCAAAACAAATAACTAAAAATGCAAAGGCTT
TTGCTGAGGCATTAGCAGCTGAAGGTTTTAAGGTTATAGGAGAGCATTTAGGTTATACTCAGAGCCATCAAGTAGTGCTG
GATGTTAGAAACTTAGGTGGAGGTGCTAAGATAGCTAAATTGTTCGAAGATGCTAACATAATAACTAATAAAAACTTATT
ACCTTATGATCCACCTAGTGCTGTAAAAGATCCTAGTGGTATAAGGTTAGGAGTACAAGAAATGACCAGGTTTGGTATGA
AAGAAGAAGAAATGAGAGAAATAGCAAAGCTAATGAGAGAAGTGGCAATTGATGGTAAAGATCCAAATGAAGTAAAGAAA
AAAGTAATTGAATTTAGAAAGAATTATTTAGAGGTCAAATACACATTCAGCGTCGATTTATCAAAGTATTCTAATGGAAA
AATGCTACCATTACTTATCTAA

Upstream 100 bases:

>100_bases
ATTTACTTGCAGCATTGGCGAATAAGGAATTAGGTAAAGCCCATTTAAGGTTAGGCAGAGGGGCCAAAGTTTAAAAGTTA
TGAAAAATTAACTTAACCAT

Downstream 100 bases:

>100_bases
CTAATATAACTGTTGTTGGTGCATTTACTGAAATAGATAAAGCAATTGAGCCAATATTTATATCACTATTTATTGATAGC
CCTCTAGCACCTAGTATAAC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSQIPKELEKVIELTREQNRWRRTEVINLIASENVMSPLAETVYMSDFMSRYAEGKPYKRYYQGTKYVDEVETLAMQLMN
EITNTKFCDLRATSGTIANAAVFRVLANPGEKALIAPVQAGAHVSHTKFGTLGALGIEHIELPYDADKMNVDVDKAIKMI
EQIKPKFIVMGGSLYLFPHPVKELAPHAHAVGAKVVYDAAHVYGLITGKAWHNPLEEGADIMTSSTHKTFPGPQGGAVFS
NEEEIFKKVADTIFPWFVSNHHLHRLPATAVTALEMKYFGEDYAKQITKNAKAFAEALAAEGFKVIGEHLGYTQSHQVVL
DVRNLGGGAKIAKLFEDANIITNKNLLPYDPPSAVKDPSGIRLGVQEMTRFGMKEEEMREIAKLMREVAIDGKDPNEVKK
KVIEFRKNYLEVKYTFSVDLSKYSNGKMLPLLI

Sequences:

>Translated_433_residues
MSQIPKELEKVIELTREQNRWRRTEVINLIASENVMSPLAETVYMSDFMSRYAEGKPYKRYYQGTKYVDEVETLAMQLMN
EITNTKFCDLRATSGTIANAAVFRVLANPGEKALIAPVQAGAHVSHTKFGTLGALGIEHIELPYDADKMNVDVDKAIKMI
EQIKPKFIVMGGSLYLFPHPVKELAPHAHAVGAKVVYDAAHVYGLITGKAWHNPLEEGADIMTSSTHKTFPGPQGGAVFS
NEEEIFKKVADTIFPWFVSNHHLHRLPATAVTALEMKYFGEDYAKQITKNAKAFAEALAAEGFKVIGEHLGYTQSHQVVL
DVRNLGGGAKIAKLFEDANIITNKNLLPYDPPSAVKDPSGIRLGVQEMTRFGMKEEEMREIAKLMREVAIDGKDPNEVKK
KVIEFRKNYLEVKYTFSVDLSKYSNGKMLPLLI
>Mature_432_residues
SQIPKELEKVIELTREQNRWRRTEVINLIASENVMSPLAETVYMSDFMSRYAEGKPYKRYYQGTKYVDEVETLAMQLMNE
ITNTKFCDLRATSGTIANAAVFRVLANPGEKALIAPVQAGAHVSHTKFGTLGALGIEHIELPYDADKMNVDVDKAIKMIE
QIKPKFIVMGGSLYLFPHPVKELAPHAHAVGAKVVYDAAHVYGLITGKAWHNPLEEGADIMTSSTHKTFPGPQGGAVFSN
EEEIFKKVADTIFPWFVSNHHLHRLPATAVTALEMKYFGEDYAKQITKNAKAFAEALAAEGFKVIGEHLGYTQSHQVVLD
VRNLGGGAKIAKLFEDANIITNKNLLPYDPPSAVKDPSGIRLGVQEMTRFGMKEEEMREIAKLMREVAIDGKDPNEVKKK
VIEFRKNYLEVKYTFSVDLSKYSNGKMLPLLI

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=427, Percent_Identity=29.5081967213115, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI261862350, Length=427, Percent_Identity=29.5081967213115, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI261862348, Length=427, Percent_Identity=29.5081967213115, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI19923315, Length=427, Percent_Identity=29.5081967213115, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI261862346, Length=422, Percent_Identity=29.6208530805687, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI22547186, Length=437, Percent_Identity=28.3752860411899, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI22547189, Length=418, Percent_Identity=28.4688995215311, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1788902, Length=404, Percent_Identity=34.6534653465347, Blast_Score=178, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI25144729, Length=434, Percent_Identity=27.4193548387097, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25144732, Length=434, Percent_Identity=27.4193548387097, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6319739, Length=395, Percent_Identity=29.873417721519, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6323087, Length=392, Percent_Identity=29.8469387755102, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640005, Length=410, Percent_Identity=29.7560975609756, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI221329721, Length=410, Percent_Identity=29.7560975609756, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_SULTO (Q971K4)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377307.1
- ProteinModelPortal:   Q971K4
- GeneID:   1459376
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1354
- NMPDR:   fig|273063.1.peg.1475
- HOGENOM:   HBG301263
- OMA:   SSTHKTF
- ProtClustDB:   PRK00011
- BioCyc:   STOK273063:ST1354-MONOMER
- BRENDA:   2.1.2.1
- GO:   GO:0005737
- HAMAP:   MF_00051_A
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 48379; Mature: 48248

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIPKELEKVIELTREQNRWRRTEVINLIASENVMSPLAETVYMSDFMSRYAEGKPYKR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YYQGTKYVDEVETLAMQLMNEITNTKFCDLRATSGTIANAAVFRVLANPGEKALIAPVQA
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCC
GAHVSHTKFGTLGALGIEHIELPYDADKMNVDVDKAIKMIEQIKPKFIVMGGSLYLFPHP
CCCCCCCCCCCHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEEEECCCC
VKELAPHAHAVGAKVVYDAAHVYGLITGKAWHNPLEEGADIMTSSTHKTFPGPQGGAVFS
HHHHCCHHHHHHHHHHHHHHHHHHEEECHHHCCHHHHCHHHHCCCCCCCCCCCCCCCCCC
NEEEIFKKVADTIFPWFVSNHHLHRLPATAVTALEMKYFGEDYAKQITKNAKAFAEALAA
CHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGFKVIGEHLGYTQSHQVVLDVRNLGGGAKIAKLFEDANIITNKNLLPYDPPSAVKDPSG
HHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
IRLGVQEMTRFGMKEEEMREIAKLMREVAIDGKDPNEVKKKVIEFRKNYLEVKYTFSVDL
CEECHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEE
SKYSNGKMLPLLI
EECCCCCEEEEEC
>Mature Secondary Structure 
SQIPKELEKVIELTREQNRWRRTEVINLIASENVMSPLAETVYMSDFMSRYAEGKPYKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YYQGTKYVDEVETLAMQLMNEITNTKFCDLRATSGTIANAAVFRVLANPGEKALIAPVQA
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCC
GAHVSHTKFGTLGALGIEHIELPYDADKMNVDVDKAIKMIEQIKPKFIVMGGSLYLFPHP
CCCCCCCCCCCHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEECCEEEECCCC
VKELAPHAHAVGAKVVYDAAHVYGLITGKAWHNPLEEGADIMTSSTHKTFPGPQGGAVFS
HHHHCCHHHHHHHHHHHHHHHHHHEEECHHHCCHHHHCHHHHCCCCCCCCCCCCCCCCCC
NEEEIFKKVADTIFPWFVSNHHLHRLPATAVTALEMKYFGEDYAKQITKNAKAFAEALAA
CHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGFKVIGEHLGYTQSHQVVLDVRNLGGGAKIAKLFEDANIITNKNLLPYDPPSAVKDPSG
HHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
IRLGVQEMTRFGMKEEEMREIAKLMREVAIDGKDPNEVKKKVIEFRKNYLEVKYTFSVDL
CEECHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEE
SKYSNGKMLPLLI
EECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479