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The map label for this gene is mcrB [H]

Identifier: 15921411

GI number: 15921411

Start: 1153684

End: 1155735

Strand: Direct

Name: mcrB [H]

Synonym: ST1153

Alternate gene names: 15921411

Gene position: 1153684-1155735 (Clockwise)

Preceding gene: 15921410

Following gene: 15921413

Centisome position: 42.81

GC content: 34.6

Gene sequence:

>2052_bases
ATGACAGATCTTAGTGAGTTATTACTTAAATATTTCCTTACTTCCTTAAATCTCATAGAAGTTGAGCCTAGAGGTAATGC
AGAAGAAACTAACAATAAAGAGGTAATGAAGATGTTGAATGAGGGATATATACCGCTTTTTTCGGCTGAAAAAATAAAGT
TCATAAAGAATCTTTCTCCAGTTTATGCACAACTCAAAGATATGGCTGATGCAATATTCAATGAGCTAAACATAAGGACT
GAACTTCCGGCTTCTTACAAAGTTGCAATTGCATTACAACTCTCCATATTGGAGACGTGGCGGAAAGTGATAGTAGAAAA
GGCAATGTTAGACGAAAGAATTGTTATAGACGAGGAAAATAAACTGGATATCTCCCCTTATGCTTTACTACTGTTATATA
GATTCATTGCAATTTCGAAATTTATGTGTACTGGTAACGAAATAGTGAAGCTTTTTAGCAAAGTCTTGGAAACACAGAAA
GAAGATAATGCTATTGTGGATATTAAAAGGCTTGAGGAGTATATAGAGAAGAAGAGGCAAGACAGAAACGAACAAAGAAG
GGCATATACAATGTATAATACGCTTTTTGCTCCATTAATACTTTCATATGCTTTAAGCGGTGCGAGTTTACTTAAATACT
ATAGGGGACTAACACAGAATCAGCTATCTTATACCTCTACGACACTCATGGAAATTCTAGAGTCTTATTTCTTTAGAGTA
ATTTACGATGATAATCGCTCCACTTTAATTTCTCTAGCTAAAGAATTAGTAGACTATTTTTCAGTTTTACACGAAATTGT
TTCTAACAAGGAACTAAAATCTATTATAAAAGAAGGTTCCCTATATTCTTACGTGTTGTCTAAGGCTAATGAACGTGGTA
TTACTGATATGAGTAAAATTTCCGTAATATGGGGTTTAATAAATGATGCTGTGATTGCTGCATTAGTCTCTTTAGAGCTT
TACGATAATGAAGAACTTAAAACTGCTTATAGGAACTTAGTTGGAGATATCACTATTTATGAATATGTAATTAAATATCC
ACAGAAAAACAAAGCTCAACAAATACAAGCTCAATATTCTCAAATCTCTACAGTCCTATGTAATAAGGGTTCACAGATCA
AGGTTGACGACCTATATCTTCCCCAAAATAATTCCCTCAATATTATATTAAGTTCGGTAAAAAACGGGAACGTGTTGTTC
ATAGGTCCTCCGGGAACTGGAAAGACTACTTTAGCAATAAGAATAGCTGAAGGGATTACTGGAAATAGAGAGTGTTACCA
AATAGTTACCGCAAATGCATTATGGTTCAGAAGGCACTTAATCGGTGGGGAAAGCCTAGAGAAAGAAACCGTTGTTTGGA
AGAGCGGTTTGCTCCTACAAGCTTACGTAAAAGCATCTAAGATAAAAGACGGATATTATTTTATAATTATAGATGAGCTG
AATAGAGCCGACATTGATAAAGCTTTTGGGGAGCTGTTTACAATATTCTCCAGCCCGTCAGCAGATGACTGGTCAATGCC
TAACAGTATTTACGAGGAAATTGAAAGATATGGAGATAACATTGATGAAATTGCAAGAGAATTTAAGAGAGTATATTCAA
AGTTAAAGAGTGAGGGAAGAGAGAACGAACCGTTAAGAAAAATAAGGGTAATAGGAACGCTCAATTTGATAGATGCCAGA
AACCTCTTCTATGTAGGAGATGCGTTAACTAGGAGGTTTGTTACAATCCAATTTGAATATCCAAAAGATGTGGAAGATCT
GGAGAAGTTCTTTACCTATTATCACCTTAATGAGAATGAAAAACAAGAGATTAGGGAGCTAGTAATGGAACTTAGACAAG
AATTCGGTAGAAGTAAGACCGTTAAGTTCAACATTTCACCTGCAAGTTTAAGGATAGCATTAAAGCTTTACTCCTCTCTT
GAGGAGAGGGATATAAACGCATTTATAAACATCTTGAAAAGTACACTAGGAACACTGAATCAGGATGTAATTAGGATGTT
TGATAATATTGTAGAGGAGTATGAAAAGAAGAAAAAAGGTATTATTAGTTAA

Upstream 100 bases:

>100_bases
AGTTTAAATTTATCTTCTCTTTAACGTTAGTTTGTGGCTACTAATTCCGAAGTTTGCATAGAGAAGCTATTACTTCGTCC
GCATAGGAGGTATATAAGAA

Downstream 100 bases:

>100_bases
TTACCTTTCATCTAAGTCCACATCTTTAACCTCTGCGTCCTTTAAATCTTCTAGTATTTTCTTCATAGTCTTCTCGTCTG
GAAAGGTTACTATTAACCCA

Product: 5-methylcytosine-specific restriction enzyme

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MTDLSELLLKYFLTSLNLIEVEPRGNAEETNNKEVMKMLNEGYIPLFSAEKIKFIKNLSPVYAQLKDMADAIFNELNIRT
ELPASYKVAIALQLSILETWRKVIVEKAMLDERIVIDEENKLDISPYALLLLYRFIAISKFMCTGNEIVKLFSKVLETQK
EDNAIVDIKRLEEYIEKKRQDRNEQRRAYTMYNTLFAPLILSYALSGASLLKYYRGLTQNQLSYTSTTLMEILESYFFRV
IYDDNRSTLISLAKELVDYFSVLHEIVSNKELKSIIKEGSLYSYVLSKANERGITDMSKISVIWGLINDAVIAALVSLEL
YDNEELKTAYRNLVGDITIYEYVIKYPQKNKAQQIQAQYSQISTVLCNKGSQIKVDDLYLPQNNSLNIILSSVKNGNVLF
IGPPGTGKTTLAIRIAEGITGNRECYQIVTANALWFRRHLIGGESLEKETVVWKSGLLLQAYVKASKIKDGYYFIIIDEL
NRADIDKAFGELFTIFSSPSADDWSMPNSIYEEIERYGDNIDEIAREFKRVYSKLKSEGRENEPLRKIRVIGTLNLIDAR
NLFYVGDALTRRFVTIQFEYPKDVEDLEKFFTYYHLNENEKQEIRELVMELRQEFGRSKTVKFNISPASLRIALKLYSSL
EERDINAFINILKSTLGTLNQDVIRMFDNIVEEYEKKKKGIIS

Sequences:

>Translated_683_residues
MTDLSELLLKYFLTSLNLIEVEPRGNAEETNNKEVMKMLNEGYIPLFSAEKIKFIKNLSPVYAQLKDMADAIFNELNIRT
ELPASYKVAIALQLSILETWRKVIVEKAMLDERIVIDEENKLDISPYALLLLYRFIAISKFMCTGNEIVKLFSKVLETQK
EDNAIVDIKRLEEYIEKKRQDRNEQRRAYTMYNTLFAPLILSYALSGASLLKYYRGLTQNQLSYTSTTLMEILESYFFRV
IYDDNRSTLISLAKELVDYFSVLHEIVSNKELKSIIKEGSLYSYVLSKANERGITDMSKISVIWGLINDAVIAALVSLEL
YDNEELKTAYRNLVGDITIYEYVIKYPQKNKAQQIQAQYSQISTVLCNKGSQIKVDDLYLPQNNSLNIILSSVKNGNVLF
IGPPGTGKTTLAIRIAEGITGNRECYQIVTANALWFRRHLIGGESLEKETVVWKSGLLLQAYVKASKIKDGYYFIIIDEL
NRADIDKAFGELFTIFSSPSADDWSMPNSIYEEIERYGDNIDEIAREFKRVYSKLKSEGRENEPLRKIRVIGTLNLIDAR
NLFYVGDALTRRFVTIQFEYPKDVEDLEKFFTYYHLNENEKQEIRELVMELRQEFGRSKTVKFNISPASLRIALKLYSSL
EERDINAFINILKSTLGTLNQDVIRMFDNIVEEYEKKKKGIIS
>Mature_682_residues
TDLSELLLKYFLTSLNLIEVEPRGNAEETNNKEVMKMLNEGYIPLFSAEKIKFIKNLSPVYAQLKDMADAIFNELNIRTE
LPASYKVAIALQLSILETWRKVIVEKAMLDERIVIDEENKLDISPYALLLLYRFIAISKFMCTGNEIVKLFSKVLETQKE
DNAIVDIKRLEEYIEKKRQDRNEQRRAYTMYNTLFAPLILSYALSGASLLKYYRGLTQNQLSYTSTTLMEILESYFFRVI
YDDNRSTLISLAKELVDYFSVLHEIVSNKELKSIIKEGSLYSYVLSKANERGITDMSKISVIWGLINDAVIAALVSLELY
DNEELKTAYRNLVGDITIYEYVIKYPQKNKAQQIQAQYSQISTVLCNKGSQIKVDDLYLPQNNSLNIILSSVKNGNVLFI
GPPGTGKTTLAIRIAEGITGNRECYQIVTANALWFRRHLIGGESLEKETVVWKSGLLLQAYVKASKIKDGYYFIIIDELN
RADIDKAFGELFTIFSSPSADDWSMPNSIYEEIERYGDNIDEIAREFKRVYSKLKSEGRENEPLRKIRVIGTLNLIDARN
LFYVGDALTRRFVTIQFEYPKDVEDLEKFFTYYHLNENEKQEIRELVMELRQEFGRSKTVKFNISPASLRIALKLYSSLE
ERDINAFINILKSTLGTLNQDVIRMFDNIVEEYEKKKKGIIS

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=250, Percent_Identity=26.4, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 78838; Mature: 78707

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSELLLKYFLTSLNLIEVEPRGNAEETNNKEVMKMLNEGYIPLFSAEKIKFIKNLSP
CCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHCCCH
VYAQLKDMADAIFNELNIRTELPASYKVAIALQLSILETWRKVIVEKAMLDERIVIDEEN
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCEEEECCCC
KLDISPYALLLLYRFIAISKFMCTGNEIVKLFSKVLETQKEDNAIVDIKRLEEYIEKKRQ
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHH
DRNEQRRAYTMYNTLFAPLILSYALSGASLLKYYRGLTQNQLSYTSTTLMEILESYFFRV
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEE
IYDDNRSTLISLAKELVDYFSVLHEIVSNKELKSIIKEGSLYSYVLSKANERGITDMSKI
EEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHH
SVIWGLINDAVIAALVSLELYDNEELKTAYRNLVGDITIYEYVIKYPQKNKAQQIQAQYS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHH
QISTVLCNKGSQIKVDDLYLPQNNSLNIILSSVKNGNVLFIGPPGTGKTTLAIRIAEGIT
HHHHHHHCCCCEEEEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCEEEEEEEECCCC
GNRECYQIVTANALWFRRHLIGGESLEKETVVWKSGLLLQAYVKASKIKDGYYFIIIDEL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEECC
NRADIDKAFGELFTIFSSPSADDWSMPNSIYEEIERYGDNIDEIAREFKRVYSKLKSEGR
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
ENEPLRKIRVIGTLNLIDARNLFYVGDALTRRFVTIQFEYPKDVEDLEKFFTYYHLNENE
CCCCHHHEEEEEEEEEECCCCEEEECHHHHCEEEEEEECCCCCHHHHHHHHHHHCCCCCH
KQEIRELVMELRQEFGRSKTVKFNISPASLRIALKLYSSLEERDINAFINILKSTLGTLN
HHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDVIRMFDNIVEEYEKKKKGIIS
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDLSELLLKYFLTSLNLIEVEPRGNAEETNNKEVMKMLNEGYIPLFSAEKIKFIKNLSP
CCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHCCCCEEECHHHHHHHHCCCH
VYAQLKDMADAIFNELNIRTELPASYKVAIALQLSILETWRKVIVEKAMLDERIVIDEEN
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCEEEECCCC
KLDISPYALLLLYRFIAISKFMCTGNEIVKLFSKVLETQKEDNAIVDIKRLEEYIEKKRQ
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHH
DRNEQRRAYTMYNTLFAPLILSYALSGASLLKYYRGLTQNQLSYTSTTLMEILESYFFRV
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEE
IYDDNRSTLISLAKELVDYFSVLHEIVSNKELKSIIKEGSLYSYVLSKANERGITDMSKI
EEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHH
SVIWGLINDAVIAALVSLELYDNEELKTAYRNLVGDITIYEYVIKYPQKNKAQQIQAQYS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHH
QISTVLCNKGSQIKVDDLYLPQNNSLNIILSSVKNGNVLFIGPPGTGKTTLAIRIAEGIT
HHHHHHHCCCCEEEEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCEEEEEEEECCCC
GNRECYQIVTANALWFRRHLIGGESLEKETVVWKSGLLLQAYVKASKIKDGYYFIIIDEL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEECC
NRADIDKAFGELFTIFSSPSADDWSMPNSIYEEIERYGDNIDEIAREFKRVYSKLKSEGR
CCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
ENEPLRKIRVIGTLNLIDARNLFYVGDALTRRFVTIQFEYPKDVEDLEKFFTYYHLNENE
CCCCHHHEEEEEEEEEECCCCEEEECHHHHCEEEEEEECCCCCHHHHHHHHHHHCCCCCH
KQEIRELVMELRQEFGRSKTVKFNISPASLRIALKLYSSLEERDINAFINILKSTLGTLN
HHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDVIRMFDNIVEEYEKKKKGIIS
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]