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The map label for this gene is radA

Identifier: 15920489

GI number: 15920489

Start: 316394

End: 317368

Strand: Reverse

Name: radA

Synonym: ST0297

Alternate gene names: 15920489

Gene position: 317368-316394 (Counterclockwise)

Preceding gene: 15920490

Following gene: 15920488

Centisome position: 11.78

GC content: 37.33

Gene sequence:

>975_bases
GTGAATGATATGTCTTCTGATGGGAAGAAAGTAAAAAGTCTCTCAGATCTTCCTGGAGTAGGACAGAGTATTCTTAACAA
ACTTATTGAAGCTGGGTATTCGTCATTAGAAGCAGTTGCTGTAGCTTCTCCTCAAGATTTGAGTGTAGCTGCAGGAATAC
CACTAACTACAGCCCAAAGAATAATTAAAGAGGCGAGAGAGGCTTTAGATATAAGATTTAAAACAGCCTTGGAAGTAAAG
AAGGAGAGGATAAATACTAAGAAAATAACTACTGGTAGTCAAGCTTTAGACGGACTTTTAGGCGGAGGAATAGAGACAAG
AACAATGACTGAACTCTTCGGAGAGTTTGGCTCTGGTAAAACTCAATTATGCCATCAGTTAAGTGTTAATGTTCAATTAC
CGTTAGAAAAAGGCGGATTAGGAGGTAAAGCTGTTTATATAGATACTGAGGGTACTTTTAGATGGGAAAGAATAGAAGCT
ATGTCAAAGGCTATAGGGTTAGAACCAGATAGTGCAATGAATAATATATATTATATGAGAGCGATTAACAGTGATCATCA
AATGGCAATAGTTGATGATCTTCAAGAACTCATTAGCAAAGATCCAGCAATTAAACTAGTAATTGTAGATTCTGTAACTT
CACACTTTAGGGCTGAATTCCCTGGGAGAGAAAATTTAGCTGTGAGACAGCAAAAATTAAACAAACACTTACATCAATTA
GTAAGATTAGCGGAAATGTATGACCTTGCGGTTATAATAACAAATCAAGTTATGGCGAGGCCAGATATGTTTTATGGTGA
TCCTACAGTGGCTGTAGGTGGTCATACTCTATATCATGTTCCAGGTATAAGAGTTCAGCTTAAGAAAAGTAGAGGCAATA
AAAGAATAGCTAGAATAGTTGATGCTCCTCATTTGCCAGAAGGAGAAGTAGTATTTGCGATTACGGAAGAGGGAGTAAGA
GACGCTGAAGAGTAA

Upstream 100 bases:

>100_bases
TCAGTTAAGATAATACCAGAATTAGATATAGGTTGGTATTTAAGAAAGGGAGGATAAAAGTTTATTAGGAGTAAATAAGA
AGAGTAACATTTAGAAATTG

Downstream 100 bases:

>100_bases
TTTTTAATCCATCTCTTAATTTATTTCACTTATGGATATTCTCTGGGCTCCTTGGAGGTCAAAATATGTCTCTGAAGCTT
CAAAAAATAAGTCCTCCTCG

Product: DNA repair and recombination protein RadA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNDMSSDGKKVKSLSDLPGVGQSILNKLIEAGYSSLEAVAVASPQDLSVAAGIPLTTAQRIIKEAREALDIRFKTALEVK
KERINTKKITTGSQALDGLLGGGIETRTMTELFGEFGSGKTQLCHQLSVNVQLPLEKGGLGGKAVYIDTEGTFRWERIEA
MSKAIGLEPDSAMNNIYYMRAINSDHQMAIVDDLQELISKDPAIKLVIVDSVTSHFRAEFPGRENLAVRQQKLNKHLHQL
VRLAEMYDLAVIITNQVMARPDMFYGDPTVAVGGHTLYHVPGIRVQLKKSRGNKRIARIVDAPHLPEGEVVFAITEEGVR
DAEE

Sequences:

>Translated_324_residues
MNDMSSDGKKVKSLSDLPGVGQSILNKLIEAGYSSLEAVAVASPQDLSVAAGIPLTTAQRIIKEAREALDIRFKTALEVK
KERINTKKITTGSQALDGLLGGGIETRTMTELFGEFGSGKTQLCHQLSVNVQLPLEKGGLGGKAVYIDTEGTFRWERIEA
MSKAIGLEPDSAMNNIYYMRAINSDHQMAIVDDLQELISKDPAIKLVIVDSVTSHFRAEFPGRENLAVRQQKLNKHLHQL
VRLAEMYDLAVIITNQVMARPDMFYGDPTVAVGGHTLYHVPGIRVQLKKSRGNKRIARIVDAPHLPEGEVVFAITEEGVR
DAEE
>Mature_324_residues
MNDMSSDGKKVKSLSDLPGVGQSILNKLIEAGYSSLEAVAVASPQDLSVAAGIPLTTAQRIIKEAREALDIRFKTALEVK
KERINTKKITTGSQALDGLLGGGIETRTMTELFGEFGSGKTQLCHQLSVNVQLPLEKGGLGGKAVYIDTEGTFRWERIEA
MSKAIGLEPDSAMNNIYYMRAINSDHQMAIVDDLQELISKDPAIKLVIVDSVTSHFRAEFPGRENLAVRQQKLNKHLHQL
VRLAEMYDLAVIITNQVMARPDMFYGDPTVAVGGHTLYHVPGIRVQLKKSRGNKRIARIVDAPHLPEGEVVFAITEEGVR
DAEE

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family

Homologues:

Organism=Homo sapiens, GI19924133, Length=309, Percent_Identity=44.0129449838188, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI23238219, Length=310, Percent_Identity=40.3225806451613, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI256017143, Length=310, Percent_Identity=40.3225806451613, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI256017141, Length=310, Percent_Identity=40.3225806451613, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI256017145, Length=227, Percent_Identity=43.6123348017621, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI17402896, Length=328, Percent_Identity=32.6219512195122, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI10835029, Length=335, Percent_Identity=28.3582089552239, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI46255039, Length=335, Percent_Identity=28.3582089552239, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI19924117, Length=335, Percent_Identity=28.3582089552239, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI153946430, Length=277, Percent_Identity=31.0469314079422, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4885659, Length=277, Percent_Identity=31.0469314079422, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI153946427, Length=277, Percent_Identity=31.0469314079422, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71997301, Length=302, Percent_Identity=44.0397350993377, Blast_Score=253, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI71997295, Length=302, Percent_Identity=44.0397350993377, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6320942, Length=309, Percent_Identity=43.6893203883495, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321027, Length=327, Percent_Identity=40.6727828746177, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6320207, Length=184, Percent_Identity=39.1304347826087, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864108, Length=313, Percent_Identity=40.2555910543131, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24651285, Length=270, Percent_Identity=42.2222222222222, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136506, Length=291, Percent_Identity=30.9278350515464, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650551, Length=207, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RADA_SULTO (Q975Y1)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_376158.1
- ProteinModelPortal:   Q975Y1
- SMR:   Q975Y1
- GeneID:   1458202
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST0297
- NMPDR:   fig|273063.1.peg.326
- HOGENOM:   HBG413235
- OMA:   FVARAYN
- ProtClustDB:   PRK04301
- BioCyc:   STOK273063:ST0297-MONOMER
- HAMAP:   MF_00348
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR020587
- PIRSF:   PIRSF005856
- SMART:   SM00382
- TIGRFAMs:   TIGR02236

Pfam domain/function: PF08423 Rad51; SSF47794 Rad51_N

EC number: NA

Molecular weight: Translated: 35530; Mature: 35530

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMSSDGKKVKSLSDLPGVGQSILNKLIEAGYSSLEAVAVASPQDLSVAAGIPLTTAQR
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHEEEECCCCCCEEECCCCHHHHHH
IIKEAREALDIRFKTALEVKKERINTKKITTGSQALDGLLGGGIETRTMTELFGEFGSGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHCCCH
TQLCHQLSVNVQLPLEKGGLGGKAVYIDTEGTFRWERIEAMSKAIGLEPDSAMNNIYYMR
HHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHCCEEEEE
AINSDHQMAIVDDLQELISKDPAIKLVIVDSVTSHFRAEFPGRENLAVRQQKLNKHLHQL
EECCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VRLAEMYDLAVIITNQVMARPDMFYGDPTVAVGGHTLYHVPGIRVQLKKSRGNKRIARIV
HHHHHHHHHHHEEHHHHHHCCCCEECCCEEEECCCEEEECCCCEEEEECCCCCHHHHHHH
DAPHLPEGEVVFAITEEGVRDAEE
CCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MNDMSSDGKKVKSLSDLPGVGQSILNKLIEAGYSSLEAVAVASPQDLSVAAGIPLTTAQR
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHEEEECCCCCCEEECCCCHHHHHH
IIKEAREALDIRFKTALEVKKERINTKKITTGSQALDGLLGGGIETRTMTELFGEFGSGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCCCCCHHHHHHHHHHHCCCH
TQLCHQLSVNVQLPLEKGGLGGKAVYIDTEGTFRWERIEAMSKAIGLEPDSAMNNIYYMR
HHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHCCEEEEE
AINSDHQMAIVDDLQELISKDPAIKLVIVDSVTSHFRAEFPGRENLAVRQQKLNKHLHQL
EECCCCCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
VRLAEMYDLAVIITNQVMARPDMFYGDPTVAVGGHTLYHVPGIRVQLKKSRGNKRIARIV
HHHHHHHHHHHEEHHHHHHCCCCEECCCEEEECCCEEEECCCCEEEEECCCCCHHHHHHH
DAPHLPEGEVVFAITEEGVRDAEE
CCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572479