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The map label for this gene is rlmN

Identifier: 159185320

GI number: 159185320

Start: 2657288

End: 2658460

Strand: Reverse

Name: rlmN

Synonym: Atu2673

Alternate gene names: 159185320

Gene position: 2658460-2657288 (Counterclockwise)

Preceding gene: 159185445

Following gene: 159185319

Centisome position: 93.56

GC content: 60.36

Gene sequence:

>1173_bases
ATGTCCGGCAAGCCGTCGCTGATCGGCCTGACGCGTGAGGAAATGGGCGAGGCGCTGGCCGAGATCGGTGTGCCGCAGAA
GCAGGTGAAAATGCGCGTCAGCCAGTTGTGGAACTGGCTCTACGTGCGTGGCGTCTCCGATTTCGACAATATGACAAATG
TCGCCAAGGAACTTCGCGAGAAGCTGAAAGCGGCCTTCACCATCGCTCGCCCGGAAATCGTCGAGGAGCAGATCTCCAAT
GACGGCACCCGCAAATGGCTGATGCGTTTTCCGCCGCGCGGCGCGGGCCGTCCCGTCGAGATCGAGACGGTCTATATTCC
CGAAGAGGGACGCGGCACGTTGTGCATATCGAGCCAGGTGGGCTGTTCGCTGACCTGCTCTTTCTGTCACACCGGCACGC
AGCGGCTGGTGCGCAACCTGACGGCGGAGGAAATTCTCTCCCAGCTTTTGCTCGCCCGCGACCGGCTGGGTGACTTCCCT
GATGGGTCGACGCCGGTCGGCGCCTATGTGCCGAGCGAAGGCCGCAAGGTCTCCAACATCGTGATGATGGGCATGGGCGA
GCCGCTCTATAATTTCGAGCATGTGAAGACCGCGCTTCTGATCGCCACCGATGGTGACGGCCTGTCGCTTTCCAAGCGTC
GGGTGACGCTCTCCACATCAGGCGTCGTGCCGGAAATCTTCCGCACGGGCGACGAGATCGGCGTGATGCTGGCGATTTCG
CTGCATGCGGTGCGCGACGATCTGCGCGACATGCTGGTGCCGATCAACAAGAAATATCCCCTGAAGGAGCTGATCGAGGC
CTGCCGCAACTATCCGGGTGTTTCCAATGCCCGCCGCATCACCTTCGAATACGTGATGCTGAAGGATGTCAATGACAGCC
TGGAAGACGCCAAGATGCTGGTGCAGCTTCTGAAGGGCGTTCCGGCCAAGATCAATCTCATTCCGTTCAATCCCTGGCCC
GGTACGAACTACCAGTGCTCGGAGTGGGCGCAGATCGAAAAATTCGCCGACTTCATCAATCAGGCGGGTTATGCGTCGCC
GATCCGCACGCCGCGCGGCCGCGACATTCTCGCCGCCTGCGGCCAGTTGAAGTCGGAATCTGAACGCATGCGCAAGACCG
AAAGACTGGCTTTTGAGGCGATGATGATCGCCAACCACGGCGCAGATGACTGA

Upstream 100 bases:

>100_bases
GGTCGTGCGATGCATGGAACGATGGGATAACGTGATGTCCGTAATGCAAGCTCCTGCCGGCCTGGCAGTCAAAGCCCCGT
TGATCGCCAACAGCGATCTC

Downstream 100 bases:

>100_bases
ATAAGCCGCGTTTGCGCCATAAACCTGGCTTTCCGGCGGTTCTCCGATTGATTTCGGTCTGTCTTGTTCCTAGAAAGACG
AAAACAGGGAACCAACAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSGKPSLIGLTREEMGEALAEIGVPQKQVKMRVSQLWNWLYVRGVSDFDNMTNVAKELREKLKAAFTIARPEIVEEQISN
DGTRKWLMRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCSLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFP
DGSTPVGAYVPSEGRKVSNIVMMGMGEPLYNFEHVKTALLIATDGDGLSLSKRRVTLSTSGVVPEIFRTGDEIGVMLAIS
LHAVRDDLRDMLVPINKKYPLKELIEACRNYPGVSNARRITFEYVMLKDVNDSLEDAKMLVQLLKGVPAKINLIPFNPWP
GTNYQCSEWAQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMMIANHGADD

Sequences:

>Translated_390_residues
MSGKPSLIGLTREEMGEALAEIGVPQKQVKMRVSQLWNWLYVRGVSDFDNMTNVAKELREKLKAAFTIARPEIVEEQISN
DGTRKWLMRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCSLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFP
DGSTPVGAYVPSEGRKVSNIVMMGMGEPLYNFEHVKTALLIATDGDGLSLSKRRVTLSTSGVVPEIFRTGDEIGVMLAIS
LHAVRDDLRDMLVPINKKYPLKELIEACRNYPGVSNARRITFEYVMLKDVNDSLEDAKMLVQLLKGVPAKINLIPFNPWP
GTNYQCSEWAQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMMIANHGADD
>Mature_389_residues
SGKPSLIGLTREEMGEALAEIGVPQKQVKMRVSQLWNWLYVRGVSDFDNMTNVAKELREKLKAAFTIARPEIVEEQISND
GTRKWLMRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCSLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFPD
GSTPVGAYVPSEGRKVSNIVMMGMGEPLYNFEHVKTALLIATDGDGLSLSKRRVTLSTSGVVPEIFRTGDEIGVMLAISL
HAVRDDLRDMLVPINKKYPLKELIEACRNYPGVSNARRITFEYVMLKDVNDSLEDAKMLVQLLKGVPAKINLIPFNPWPG
TNYQCSEWAQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSESERMRKTERLAFEAMMIANHGADD

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=386, Percent_Identity=46.1139896373057, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_AGRT5 (Q7CWI1)

Other databases:

- EMBL:   AE007869
- RefSeq:   NP_355610.2
- STRING:   Q7CWI1
- GeneID:   1134711
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2673
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK864022
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 43485; Mature: 43354

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKPSLIGLTREEMGEALAEIGVPQKQVKMRVSQLWNWLYVRGVSDFDNMTNVAKELRE
CCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHH
KLKAAFTIARPEIVEEQISNDGTRKWLMRFPPRGAGRPVEIETVYIPEEGRGTLCISSQV
HHHHHHHHCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEEECCC
GCSLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFPDGSTPVGAYVPSEGRKVSNI
CCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
VMMGMGEPLYNFEHVKTALLIATDGDGLSLSKRRVTLSTSGVVPEIFRTGDEIGVMLAIS
EEEECCCCCCCHHHHEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHCCCHHEEEHHHH
LHAVRDDLRDMLVPINKKYPLKELIEACRNYPGVSNARRITFEYVMLKDVNDSLEDAKML
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCHHHHHHH
VQLLKGVPAKINLIPFNPWPGTNYQCSEWAQIEKFADFINQAGYASPIRTPRGRDILAAC
HHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
GQLKSESERMRKTERLAFEAMMIANHGADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGKPSLIGLTREEMGEALAEIGVPQKQVKMRVSQLWNWLYVRGVSDFDNMTNVAKELRE
CCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHH
KLKAAFTIARPEIVEEQISNDGTRKWLMRFPPRGAGRPVEIETVYIPEEGRGTLCISSQV
HHHHHHHHCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCEEEEECCC
GCSLTCSFCHTGTQRLVRNLTAEEILSQLLLARDRLGDFPDGSTPVGAYVPSEGRKVSNI
CCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEE
VMMGMGEPLYNFEHVKTALLIATDGDGLSLSKRRVTLSTSGVVPEIFRTGDEIGVMLAIS
EEEECCCCCCCHHHHEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHCCCHHEEEHHHH
LHAVRDDLRDMLVPINKKYPLKELIEACRNYPGVSNARRITFEYVMLKDVNDSLEDAKML
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCHHHHHHH
VQLLKGVPAKINLIPFNPWPGTNYQCSEWAQIEKFADFINQAGYASPIRTPRGRDILAAC
HHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
GQLKSESERMRKTERLAFEAMMIANHGADD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743194; 11743193