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The map label for this gene is opuAB [H]

Identifier: 159185138

GI number: 159185138

Start: 2251983

End: 2253014

Strand: Reverse

Name: opuAB [H]

Synonym: Atu2280

Alternate gene names: 159185138

Gene position: 2253014-2251983 (Counterclockwise)

Preceding gene: 159185139

Following gene: 15889554

Centisome position: 79.29

GC content: 59.69

Gene sequence:

>1032_bases
ATGGATCTGCACCGCGCCCGGCAAAGACGATGCCGGCCGTCGCTTCTGTATTGGAGCAATTCCGGTGACAATAGATCACT
GAAAAAGCTCCGCTTCTTTGTTGTCTGCGCAATTCCGGACGCAAAACCGCTCTGCATTTTTGCCGGAATTGCTCTCATTC
ACGAAAGGTCTTTTCTTACCGTGGAATGGCTCAGCGCTCCTGAAAACCGCCTGCCTATCGGCCGATATGCCAAGGAGGCC
ATTGACTGGCTGACCGGCAATCTCGCTTTTTTCTTCGATTGGCTCTCTTTCATTTTCCAAAGCGTCATCAACGCCCTGCT
CTACGTGCTGCAGGCGCCGCATCCGCTGATCATCGTCGCGATCGTGACGGCGCTTTCGGCCTGGACGCGCCGCTCGGCCG
GCATGCCGATCTTCACCGCACTCGGTCTGTTGCTCATCATCAATCTCGGCTACTGGAAAGCGACGACTGAGACGCTGGCC
CTGGTCATCGCCGCCAGCGCCGTGTGCATGATCATCGGCATACCGCTCGGCATATTGGCGGCACGGCGCAAGTGGATCTA
TGCCGGCATGCGCCCGGCGCTCGATCTGATGCAGACCATTCCCACCTTCGTTTATCTCATTCCCGCGCTGGTGCTGTTCG
GCCTCGGCATGGTTCCGGGGCTGATCGCAACCGTCATCTTCGCCATTCCCGCTCCCGTCCGGCTGACGCGCCTCGGCATC
GTCTCGACGCCGCCGGCGCTGGTGGAGGCGGCCGTCGCCTTCGGCGCAACGCCATCGCAGGTGCTGCGCAAGGTCGAACT
TCCATTTGCCGCGCCGCAGATCATGGCCGGTCTCACCCAGACGATCATGCTGTCGCTATCGATGGTGGTCATTTCCGCCC
TGGTGGGTGCCAATGGTCTCGGCGTGCCTGTTGTGCGCGCCTTGAACACGGTCAACATCGCCATGGGTTTCGAAGCAGGG
CTTTGCATCGTCATATTGGCGATCGTTCTCGACAGGCTTTTCCGCCTTCCCGGTACGGAGGATGATCTATGA

Upstream 100 bases:

>100_bases
GATGCATGACTGCACCCGGCCTGCGCGGCAGGTCGGATGCAGTGGGAAATACGATGCCCATGCATGACCTTGCCCGCGAA
TTTTTCTCGCATCGAAGGCC

Downstream 100 bases:

>100_bases
GTGACGCCATTATCTTCGGAAATGTCGATATCGTCTTCGGCGACCGGCCGGACGCGGCGCTGGCGCTGATAGACAAGGGC
AGTACGCGCGACGAGATCAA

Product: ABC transporter, membrane spanning protein (proline/glycine betaine)

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDLHRARQRRCRPSLLYWSNSGDNRSLKKLRFFVVCAIPDAKPLCIFAGIALIHERSFLTVEWLSAPENRLPIGRYAKEA
IDWLTGNLAFFFDWLSFIFQSVINALLYVLQAPHPLIIVAIVTALSAWTRRSAGMPIFTALGLLLIINLGYWKATTETLA
LVIAASAVCMIIGIPLGILAARRKWIYAGMRPALDLMQTIPTFVYLIPALVLFGLGMVPGLIATVIFAIPAPVRLTRLGI
VSTPPALVEAAVAFGATPSQVLRKVELPFAAPQIMAGLTQTIMLSLSMVVISALVGANGLGVPVVRALNTVNIAMGFEAG
LCIVILAIVLDRLFRLPGTEDDL

Sequences:

>Translated_343_residues
MDLHRARQRRCRPSLLYWSNSGDNRSLKKLRFFVVCAIPDAKPLCIFAGIALIHERSFLTVEWLSAPENRLPIGRYAKEA
IDWLTGNLAFFFDWLSFIFQSVINALLYVLQAPHPLIIVAIVTALSAWTRRSAGMPIFTALGLLLIINLGYWKATTETLA
LVIAASAVCMIIGIPLGILAARRKWIYAGMRPALDLMQTIPTFVYLIPALVLFGLGMVPGLIATVIFAIPAPVRLTRLGI
VSTPPALVEAAVAFGATPSQVLRKVELPFAAPQIMAGLTQTIMLSLSMVVISALVGANGLGVPVVRALNTVNIAMGFEAG
LCIVILAIVLDRLFRLPGTEDDL
>Mature_343_residues
MDLHRARQRRCRPSLLYWSNSGDNRSLKKLRFFVVCAIPDAKPLCIFAGIALIHERSFLTVEWLSAPENRLPIGRYAKEA
IDWLTGNLAFFFDWLSFIFQSVINALLYVLQAPHPLIIVAIVTALSAWTRRSAGMPIFTALGLLLIINLGYWKATTETLA
LVIAASAVCMIIGIPLGILAARRKWIYAGMRPALDLMQTIPTFVYLIPALVLFGLGMVPGLIATVIFAIPAPVRLTRLGI
VSTPPALVEAAVAFGATPSQVLRKVELPFAAPQIMAGLTQTIMLSLSMVVISALVGANGLGVPVVRALNTVNIAMGFEAG
LCIVILAIVLDRLFRLPGTEDDL

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=272, Percent_Identity=43.0147058823529, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1788449, Length=179, Percent_Identity=32.9608938547486, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788451, Length=204, Percent_Identity=30.8823529411765, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 37283; Mature: 37283

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHRARQRRCRPSLLYWSNSGDNRSLKKLRFFVVCAIPDAKPLCIFAGIALIHERSFLT
CCCHHHHHHCCCCCEEEECCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCEE
VEWLSAPENRLPIGRYAKEAIDWLTGNLAFFFDWLSFIFQSVINALLYVLQAPHPLIIVA
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IVTALSAWTRRSAGMPIFTALGLLLIINLGYWKATTETLALVIAASAVCMIIGIPLGILA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRKWIYAGMRPALDLMQTIPTFVYLIPALVLFGLGMVPGLIATVIFAIPAPVRLTRLGI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
VSTPPALVEAAVAFGATPSQVLRKVELPFAAPQIMAGLTQTIMLSLSMVVISALVGANGL
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVPVVRALNTVNIAMGFEAGLCIVILAIVLDRLFRLPGTEDDL
CHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDLHRARQRRCRPSLLYWSNSGDNRSLKKLRFFVVCAIPDAKPLCIFAGIALIHERSFLT
CCCHHHHHHCCCCCEEEECCCCCCHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCEE
VEWLSAPENRLPIGRYAKEAIDWLTGNLAFFFDWLSFIFQSVINALLYVLQAPHPLIIVA
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IVTALSAWTRRSAGMPIFTALGLLLIINLGYWKATTETLALVIAASAVCMIIGIPLGILA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRKWIYAGMRPALDLMQTIPTFVYLIPALVLFGLGMVPGLIATVIFAIPAPVRLTRLGI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
VSTPPALVEAAVAFGATPSQVLRKVELPFAAPQIMAGLTQTIMLSLSMVVISALVGANGL
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GVPVVRALNTVNIAMGFEAGLCIVILAIVLDRLFRLPGTEDDL
CHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]