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The map label for this gene is leuA [H]

Identifier: 159185129

GI number: 159185129

Start: 2236659

End: 2238368

Strand: Reverse

Name: leuA [H]

Synonym: Atu2264

Alternate gene names: 159185129

Gene position: 2238368-2236659 (Counterclockwise)

Preceding gene: 159185130

Following gene: 159185128

Centisome position: 78.77

GC content: 58.3

Gene sequence:

>1710_bases
ATGGACGGCAAGATCACCACTATTTCCAAGGGCATGACCGACGCGAGCGTGAAGTATCGCCCTTACCCGACCATCAACAT
TCCTGACCGCACATGGCCGGGCAAGACCATCGACAAGGCGCCGATCTGGTGTTCGGTGGATCTGCGCGACGGCAACCAGT
CGCTGGTCAACCCCATGGGCCACGACCGCAAGGCCCGCATGTTCAAGCTGCTGCTCGAAATGGGCTTCAAGGAAATCGAA
ATCGGTTTCCCCTCCGCCTCGCAGACGGATTTCGATTTCGCCCGCTGGTGTGTGGAAGAAGGCAATGTGCCTGATGATGT
ATCCCTGCAGGTGCTGGTGCAGTGCCGCCCGGAACTGATCACCCGCACGTTTGAAGCGTTGGAAGGCGCCAATAAGCCGA
TTATCCATTTCTACAACTCCACCTCGGAATTGCAGCGGCGCGTGGTGTTCGGCAAGGATGTGCACGGCATCAAGCAGATC
GCCGTCGATGCGGCCAAGATGATCACCGACATGGCGGCCAAGGCCGGCGGCGGTTTCCGCTTCGAATATTCGCCTGAGAG
CTTTACCGGGACCGAGCTGGAAGTGGCGCTGGAAATCTGCAACGCCGTGGTCGAGGTGGTGAAGCCGACGGCGGATAACA
AGCTGATCCTGAACCTGCCTTCCACCGTTGAGATGGCGACGCCGAACATCTATGCCGACCAGATCGAATGGATGTGCCGT
AACATCGACAATCGCGAAAACGTCATCATCTCGCTGCATCCGCATAATGACCGTGGCACGGGCATCGCTGCGACCGAACT
GGCTCTGATGGCCGGCGCAGACCGCGTCGAAGGCACCCTGTTCGGCAATGGCGAGCGCACGGGCAATGTCGATGTCGTGA
CGCTGGCGCTGAACATGTATACGCAGGGCGTGGATCCGGAACTGGATTGCCGCGATATCGAGCGCATCAAGGCGGTCTAT
GAATATTCCAACGAGATGACGATCCCCGAGCGCCACCCCTATGTCGGCGAACTGGTCTATACGGCGTTCTCCGGCTCGCA
TCAGGATGCGATCAACAAGGGCATGAAGGCGATCAAGGTCGCCAATCACCCGGTGTGGGAAGTACCCTATCTGCCGATCG
ATCCGAAGGATGTCGGTCGTTCCTACGAGGCGATCATTCGCATCAATTCGCAGTCCGGCAAGGGCGGCATCGCCTATATT
CTGCAGCAGGATTACGGCATCAACCTGCCGCGTAACCTGCAGGTGGAATTCCGTGAGGACATCCAGCGCATTACCGATGA
AGAGGGTGTTGAACTGCCGGCCAAGCGCATCTATGAGCGTTTTATCGAGCGTTACGTGACGCAGCCGAATGCCCGCATCA
AGTTCGTGGATCACCACACCTATCCGGCCGGCGATTTCAAGGGCGTGCGCATCGTCGCCGCCGAAATTACCGACAATGGC
GAGGTGAAGCGCATCGAGGGCAAGGGAACCGGTCCGATTGACGGGTTCATCAATGCGCTGTCGGTCTATCTCGGGGTTGA
TCTGTCGGTGAATGATTATTCGGAACATTCGCTGCAGCACGGCTCCAATGCATCGGCCATCGCCTATGTCGAGATGGAGC
ATCCGGGCGGGAAGCTGTTCGGGGCGGGTGTTAATACGAATATTGTGGCTGCGTCGCTGGAGGCGATCGTTTCGGCGGCC
AATCGGGTGTTGGAAGAGCGGGCGAAGTAA

Upstream 100 bases:

>100_bases
TCATCTCGAAATCTCATTTTATACTGGATTTAGGCTCTCAAGAGCCGCGCATCCGCCGATGGCATCAAGACGCCCGCGGA
CTGCGCAAGGAGGACGCGAC

Downstream 100 bases:

>100_bases
GAAGCCTCAAGGGTGGGGTTTACCCCCCTCTGCCCTGCCGGGCATCTCCCCCACAAGGGGGGAGATCGACTCGCGGTCAG
GTCTCGGCCATCTCTACATT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MDGKITTISKGMTDASVKYRPYPTINIPDRTWPGKTIDKAPIWCSVDLRDGNQSLVNPMGHDRKARMFKLLLEMGFKEIE
IGFPSASQTDFDFARWCVEEGNVPDDVSLQVLVQCRPELITRTFEALEGANKPIIHFYNSTSELQRRVVFGKDVHGIKQI
AVDAAKMITDMAAKAGGGFRFEYSPESFTGTELEVALEICNAVVEVVKPTADNKLILNLPSTVEMATPNIYADQIEWMCR
NIDNRENVIISLHPHNDRGTGIAATELALMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGVDPELDCRDIERIKAVY
EYSNEMTIPERHPYVGELVYTAFSGSHQDAINKGMKAIKVANHPVWEVPYLPIDPKDVGRSYEAIIRINSQSGKGGIAYI
LQQDYGINLPRNLQVEFREDIQRITDEEGVELPAKRIYERFIERYVTQPNARIKFVDHHTYPAGDFKGVRIVAAEITDNG
EVKRIEGKGTGPIDGFINALSVYLGVDLSVNDYSEHSLQHGSNASAIAYVEMEHPGGKLFGAGVNTNIVAASLEAIVSAA
NRVLEERAK

Sequences:

>Translated_569_residues
MDGKITTISKGMTDASVKYRPYPTINIPDRTWPGKTIDKAPIWCSVDLRDGNQSLVNPMGHDRKARMFKLLLEMGFKEIE
IGFPSASQTDFDFARWCVEEGNVPDDVSLQVLVQCRPELITRTFEALEGANKPIIHFYNSTSELQRRVVFGKDVHGIKQI
AVDAAKMITDMAAKAGGGFRFEYSPESFTGTELEVALEICNAVVEVVKPTADNKLILNLPSTVEMATPNIYADQIEWMCR
NIDNRENVIISLHPHNDRGTGIAATELALMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGVDPELDCRDIERIKAVY
EYSNEMTIPERHPYVGELVYTAFSGSHQDAINKGMKAIKVANHPVWEVPYLPIDPKDVGRSYEAIIRINSQSGKGGIAYI
LQQDYGINLPRNLQVEFREDIQRITDEEGVELPAKRIYERFIERYVTQPNARIKFVDHHTYPAGDFKGVRIVAAEITDNG
EVKRIEGKGTGPIDGFINALSVYLGVDLSVNDYSEHSLQHGSNASAIAYVEMEHPGGKLFGAGVNTNIVAASLEAIVSAA
NRVLEERAK
>Mature_569_residues
MDGKITTISKGMTDASVKYRPYPTINIPDRTWPGKTIDKAPIWCSVDLRDGNQSLVNPMGHDRKARMFKLLLEMGFKEIE
IGFPSASQTDFDFARWCVEEGNVPDDVSLQVLVQCRPELITRTFEALEGANKPIIHFYNSTSELQRRVVFGKDVHGIKQI
AVDAAKMITDMAAKAGGGFRFEYSPESFTGTELEVALEICNAVVEVVKPTADNKLILNLPSTVEMATPNIYADQIEWMCR
NIDNRENVIISLHPHNDRGTGIAATELALMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGVDPELDCRDIERIKAVY
EYSNEMTIPERHPYVGELVYTAFSGSHQDAINKGMKAIKVANHPVWEVPYLPIDPKDVGRSYEAIIRINSQSGKGGIAYI
LQQDYGINLPRNLQVEFREDIQRITDEEGVELPAKRIYERFIERYVTQPNARIKFVDHHTYPAGDFKGVRIVAAEITDNG
EVKRIEGKGTGPIDGFINALSVYLGVDLSVNDYSEHSLQHGSNASAIAYVEMEHPGGKLFGAGVNTNIVAASLEAIVSAA
NRVLEERAK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=524, Percent_Identity=23.4732824427481, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324682, Length=573, Percent_Identity=47.1204188481675, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6324225, Length=568, Percent_Identity=45.9507042253521, Blast_Score=509, Evalue=1e-145,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63111; Mature: 63111

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGKITTISKGMTDASVKYRPYPTINIPDRTWPGKTIDKAPIWCSVDLRDGNQSLVNPMG
CCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCC
HDRKARMFKLLLEMGFKEIEIGFPSASQTDFDFARWCVEEGNVPDDVSLQVLVQCRPELI
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCHHHH
TRTFEALEGANKPIIHFYNSTSELQRRVVFGKDVHGIKQIAVDAAKMITDMAAKAGGGFR
HHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
FEYSPESFTGTELEVALEICNAVVEVVKPTADNKLILNLPSTVEMATPNIYADQIEWMCR
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHH
NIDNRENVIISLHPHNDRGTGIAATELALMAGADRVEGTLFGNGERTGNVDVVTLALNMY
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHH
TQGVDPELDCRDIERIKAVYEYSNEMTIPERHPYVGELVYTAFSGSHQDAINKGMKAIKV
HCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCCHHHHHHCHHEEEE
ANHPVWEVPYLPIDPKDVGRSYEAIIRINSQSGKGGIAYILQQDYGINLPRNLQVEFRED
CCCCCEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHH
IQRITDEEGVELPAKRIYERFIERYVTQPNARIKFVDHHTYPAGDFKGVRIVAAEITDNG
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCC
EVKRIEGKGTGPIDGFINALSVYLGVDLSVNDYSEHSLQHGSNASAIAYVEMEHPGGKLF
CEEEECCCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCCEEEEEEEECCCCEEE
GAGVNTNIVAASLEAIVSAANRVLEERAK
ECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDGKITTISKGMTDASVKYRPYPTINIPDRTWPGKTIDKAPIWCSVDLRDGNQSLVNPMG
CCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCCCC
HDRKARMFKLLLEMGFKEIEIGFPSASQTDFDFARWCVEEGNVPDDVSLQVLVQCRPELI
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCHHHH
TRTFEALEGANKPIIHFYNSTSELQRRVVFGKDVHGIKQIAVDAAKMITDMAAKAGGGFR
HHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
FEYSPESFTGTELEVALEICNAVVEVVKPTADNKLILNLPSTVEMATPNIYADQIEWMCR
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHH
NIDNRENVIISLHPHNDRGTGIAATELALMAGADRVEGTLFGNGERTGNVDVVTLALNMY
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHH
TQGVDPELDCRDIERIKAVYEYSNEMTIPERHPYVGELVYTAFSGSHQDAINKGMKAIKV
HCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCCHHHHHHCHHEEEE
ANHPVWEVPYLPIDPKDVGRSYEAIIRINSQSGKGGIAYILQQDYGINLPRNLQVEFRED
CCCCCEECCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHH
IQRITDEEGVELPAKRIYERFIERYVTQPNARIKFVDHHTYPAGDFKGVRIVAAEITDNG
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCC
EVKRIEGKGTGPIDGFINALSVYLGVDLSVNDYSEHSLQHGSNASAIAYVEMEHPGGKLF
CEEEECCCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCCEEEEEEEECCCCEEE
GAGVNTNIVAASLEAIVSAANRVLEERAK
ECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]