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The map label for this gene is pncA [H]

Identifier: 159185035

GI number: 159185035

Start: 2028591

End: 2029217

Strand: Direct

Name: pncA [H]

Synonym: Atu2066

Alternate gene names: 159185035

Gene position: 2028591-2029217 (Clockwise)

Preceding gene: 15889349

Following gene: 15889353

Centisome position: 71.39

GC content: 59.97

Gene sequence:

>627_bases
ATGAAGGCGCTGCTGCTCATCGATATTCAGAACGGGTTTTGCCCGGGTGGCAATCTGGCCGTTACCGATGGAGACGCGGT
CGTACCCATCGCCAATGCCCTGATCGACAATGGCGGCTATGATCTCATCGTCGCCTCGCAGGACTGGCACCCGGAAAATC
ACGGCAGCTTCGCCTCGCAGCATCCCGGCAAGCAACCCTTCGACATGGGCGAACTTTCCGGCAAGCCGCAGATGATGTGG
CCGGACCATTGCGTGCAGGGTACGCCGGATGCGGAATTTCACCCCGACCTGAATATGGAAGCCTTCGATTACATCCAGCA
GAAGGGCGAGAACCCCGCCATCGACAGCTATTCCGCCTTTCGCGACAATGACCAGGTGGCCACCACAGGACTGTCCGACT
ATCTGGCCCGCCAGGGCGTGACGCAACTCGACGTCTGCGGCCTTGCCACCGATTATTGCGTCAGTTTTTCAGTGCAGGAT
GCCCTCGACATGTTGCCCGGCGTCAAGGTGCGCTTCATCGAGGATGCCAGTCGCGGCATCGACCCGCAGGGCATCAAAGC
CGCGGTGGCAGCCATGCGGGAAAAAGGCGCGGTCATTCTCAAGAGCCGCGACATATTGCGGAACTAG

Upstream 100 bases:

>100_bases
CCGGCTCATGCCGGAAACACCCCTGCCTGCCGGATAGGGCGAAAGGGGCTTGATCCGCCAAAGCCGGCTTGGCATCCTGC
GAAAAAAAGGGAGGCACATC

Downstream 100 bases:

>100_bases
CGCCGCGCCCTTAGCGCAGCTTCGGTTTCTCCAGCACATCGATATCAGCAGCCGCATCGTCAAGACGCCGCAGGATCGCC
GCATGGGCATCCCTAAGGCC

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKALLLIDIQNGFCPGGNLAVTDGDAVVPIANALIDNGGYDLIVASQDWHPENHGSFASQHPGKQPFDMGELSGKPQMMW
PDHCVQGTPDAEFHPDLNMEAFDYIQQKGENPAIDSYSAFRDNDQVATTGLSDYLARQGVTQLDVCGLATDYCVSFSVQD
ALDMLPGVKVRFIEDASRGIDPQGIKAAVAAMREKGAVILKSRDILRN

Sequences:

>Translated_208_residues
MKALLLIDIQNGFCPGGNLAVTDGDAVVPIANALIDNGGYDLIVASQDWHPENHGSFASQHPGKQPFDMGELSGKPQMMW
PDHCVQGTPDAEFHPDLNMEAFDYIQQKGENPAIDSYSAFRDNDQVATTGLSDYLARQGVTQLDVCGLATDYCVSFSVQD
ALDMLPGVKVRFIEDASRGIDPQGIKAAVAAMREKGAVILKSRDILRN
>Mature_208_residues
MKALLLIDIQNGFCPGGNLAVTDGDAVVPIANALIDNGGYDLIVASQDWHPENHGSFASQHPGKQPFDMGELSGKPQMMW
PDHCVQGTPDAEFHPDLNMEAFDYIQQKGENPAIDSYSAFRDNDQVATTGLSDYLARQGVTQLDVCGLATDYCVSFSVQD
ALDMLPGVKVRFIEDASRGIDPQGIKAAVAAMREKGAVILKSRDILRN

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=207, Percent_Identity=49.7584541062802, Blast_Score=192, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI193206735, Length=231, Percent_Identity=34.6320346320346, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=231, Percent_Identity=34.6320346320346, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=222, Percent_Identity=30.6306306306306, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=209, Percent_Identity=29.6650717703349, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648247, Length=227, Percent_Identity=32.5991189427313, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22511; Mature: 22511

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLLIDIQNGFCPGGNLAVTDGDAVVPIANALIDNGGYDLIVASQDWHPENHGSFASQ
CCEEEEEECCCCCCCCCCEEEECCCEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
HPGKQPFDMGELSGKPQMMWPDHCVQGTPDAEFHPDLNMEAFDYIQQKGENPAIDSYSAF
CCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHH
RDNDQVATTGLSDYLARQGVTQLDVCGLATDYCVSFSVQDALDMLPGVKVRFIEDASRGI
CCCCCEECCCHHHHHHHCCCCEEHHHHCCHHHHEECCHHHHHHHCCCCEEEEEECHHCCC
DPQGIKAAVAAMREKGAVILKSRDILRN
CCCHHHHHHHHHHHCCEEEEECCHHHCC
>Mature Secondary Structure
MKALLLIDIQNGFCPGGNLAVTDGDAVVPIANALIDNGGYDLIVASQDWHPENHGSFASQ
CCEEEEEECCCCCCCCCCEEEECCCEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
HPGKQPFDMGELSGKPQMMWPDHCVQGTPDAEFHPDLNMEAFDYIQQKGENPAIDSYSAF
CCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHH
RDNDQVATTGLSDYLARQGVTQLDVCGLATDYCVSFSVQDALDMLPGVKVRFIEDASRGI
CCCCCEECCCHHHHHHHCCCCEEHHHHCCHHHHEECCHHHHHHHCCCCEEEEEECHHCCC
DPQGIKAAVAAMREKGAVILKSRDILRN
CCCHHHHHHHHHHHCCEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]