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The map label for this gene is psuK [H]

Identifier: 159184960

GI number: 159184960

Start: 1855312

End: 1856205

Strand: Direct

Name: psuK [H]

Synonym: Atu1875

Alternate gene names: 159184960

Gene position: 1855312-1856205 (Clockwise)

Preceding gene: 15889171

Following gene: 159184961

Centisome position: 65.29

GC content: 64.43

Gene sequence:

>894_bases
ATGATCGAGACCGAGACGGCGCCCGGCGCCAGCAATCCGGGCTCGTGGATGGAAGAGGCCGGCGGCGGTGGTTTCAACGC
CGCGCGCAATCTTTCCCGCCTCGGTTTTGAAGTCCGCATCATCGCCCCGCGCGGCGGCGACGTCACCGGCGAAGTGGTGG
CGGAAGCAGCAAGGCAGGCGGGGGTGGAAGACACGCCGTTTACCTTCCTCGACCGCCGCACGCCGAGCTACACTGCCATT
CTGGAGCGTGACGGCAATCTGGTGATCGCACTGGCCGACATGGACCTCTACAAGCTCTTTACCCCGCGCCGCCTGAAGGT
ACGCGCGGTGCGCGAGGCGATTATCGCAAGCGACTTTCTACTCTGCGATGCCAATCTGCCCGAGGATACGCTGACGGCGC
TTGGCCTCATTGCCCGCGCCTGCGAAAAGCCGCTCGCCGCCATCGCCATTTCGCCGGCCAAGGCGGTGAAGCTGAAAGCG
GCGCTCGGCGATATCGACATCCTCTTCATGAACGAGGCGGAAGCCCGCGCGCTCACCGGCGAAACGGCGGAAAATGTCCG
TGACTGGCCAAATATCCTGCGCAAGGCTGGGCTTTCCGGCGGCGTCGTCACCCGCGGCGCAAGCGAAGTCGTGGCCTTCA
ACGGGACGGAAAAAGCCATCCTCCACCCGCCCCTCATCCGCGAGGTGAAGGATGTCACCGGCGCCGGCGATGCCATGGCC
TCCGGTTATCTCGCCGCCATTGCCGAGGGAAAAACAATCAGGGAGGCCCTGAGACAGGGGGCGGCGGCGGCGGCCATTAC
CGTGCAGTCATCCTTCGCCACCTCCCAGGACCTATCAAAAGACAGTGTCGAAGCCATGTTGGGGCTTGTTCCCCAGGCCG
AAATGCTGGCATGA

Upstream 100 bases:

>100_bases
GTTTAGGCCGAATCGTCCGACTTTTCCATGCGCCAGAGAGGGAAACGCAAGCGTGAAGAAAATACTCGTTCTGGGCGGCG
CGCATATCGACAGGCGCGGC

Downstream 100 bases:

>100_bases
AGCGCTTTTAAAAACTGGAACAGATGAAATGACCCGCCCCATCTCCCCGCTCCTGCCCATCGTCTATTCTCAGGAAGTCG
CCGCCGCCAAGCAGCGCGGT

Product: carbohydrate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MIETETAPGASNPGSWMEEAGGGGFNAARNLSRLGFEVRIIAPRGGDVTGEVVAEAARQAGVEDTPFTFLDRRTPSYTAI
LERDGNLVIALADMDLYKLFTPRRLKVRAVREAIIASDFLLCDANLPEDTLTALGLIARACEKPLAAIAISPAKAVKLKA
ALGDIDILFMNEAEARALTGETAENVRDWPNILRKAGLSGGVVTRGASEVVAFNGTEKAILHPPLIREVKDVTGAGDAMA
SGYLAAIAEGKTIREALRQGAAAAAITVQSSFATSQDLSKDSVEAMLGLVPQAEMLA

Sequences:

>Translated_297_residues
MIETETAPGASNPGSWMEEAGGGGFNAARNLSRLGFEVRIIAPRGGDVTGEVVAEAARQAGVEDTPFTFLDRRTPSYTAI
LERDGNLVIALADMDLYKLFTPRRLKVRAVREAIIASDFLLCDANLPEDTLTALGLIARACEKPLAAIAISPAKAVKLKA
ALGDIDILFMNEAEARALTGETAENVRDWPNILRKAGLSGGVVTRGASEVVAFNGTEKAILHPPLIREVKDVTGAGDAMA
SGYLAAIAEGKTIREALRQGAAAAAITVQSSFATSQDLSKDSVEAMLGLVPQAEMLA
>Mature_297_residues
MIETETAPGASNPGSWMEEAGGGGFNAARNLSRLGFEVRIIAPRGGDVTGEVVAEAARQAGVEDTPFTFLDRRTPSYTAI
LERDGNLVIALADMDLYKLFTPRRLKVRAVREAIIASDFLLCDANLPEDTLTALGLIARACEKPLAAIAISPAKAVKLKA
ALGDIDILFMNEAEARALTGETAENVRDWPNILRKAGLSGGVVTRGASEVVAFNGTEKAILHPPLIREVKDVTGAGDAMA
SGYLAAIAEGKTIREALRQGAAAAAITVQSSFATSQDLSKDSVEAMLGLVPQAEMLA

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=293, Percent_Identity=27.3037542662116, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788484, Length=296, Percent_Identity=30.7432432432432, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081971, Length=295, Percent_Identity=27.1186440677966, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 31204; Mature: 31204

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETETAPGASNPGSWMEEAGGGGFNAARNLSRLGFEVRIIAPRGGDVTGEVVAEAARQA
CCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHC
GVEDTPFTFLDRRTPSYTAILERDGNLVIALADMDLYKLFTPRRLKVRAVREAIIASDFL
CCCCCCCCEECCCCCCEEEEEECCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHCEE
LCDANLPEDTLTALGLIARACEKPLAAIAISPAKAVKLKAALGDIDILFMNEAEARALTG
EECCCCCHHHHHHHHHHHHHHHCCHHEEEECCHHHEEEEEECCCEEEEEECCCHHHHHCC
ETAENVRDWPNILRKAGLSGGVVTRGASEVVAFNGTEKAILHPPLIREVKDVTGAGDAMA
CCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCCEEECCHHHHHHHHHCCCCHHHH
SGYLAAIAEGKTIREALRQGAAAAAITVQSSFATSQDLSKDSVEAMLGLVPQAEMLA
HHHHHHHHCCHHHHHHHHCCCCEEEEEEECHHCCHHCCCHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure
MIETETAPGASNPGSWMEEAGGGGFNAARNLSRLGFEVRIIAPRGGDVTGEVVAEAARQA
CCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHC
GVEDTPFTFLDRRTPSYTAILERDGNLVIALADMDLYKLFTPRRLKVRAVREAIIASDFL
CCCCCCCCEECCCCCCEEEEEECCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHHHHCEE
LCDANLPEDTLTALGLIARACEKPLAAIAISPAKAVKLKAALGDIDILFMNEAEARALTG
EECCCCCHHHHHHHHHHHHHHHCCHHEEEECCHHHEEEEEECCCEEEEEECCCHHHHHCC
ETAENVRDWPNILRKAGLSGGVVTRGASEVVAFNGTEKAILHPPLIREVKDVTGAGDAMA
CCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEECCCCCEEECCHHHHHHHHHCCCCHHHH
SGYLAAIAEGKTIREALRQGAAAAAITVQSSFATSQDLSKDSVEAMLGLVPQAEMLA
HHHHHHHHCCHHHHHHHHCCCCEEEEEEECHHCCHHCCCHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]