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The map label for this gene is cysG [H]

Identifier: 159184766

GI number: 159184766

Start: 1449873

End: 1451336

Strand: Direct

Name: cysG [H]

Synonym: Atu1454

Alternate gene names: 159184766

Gene position: 1449873-1451336 (Clockwise)

Preceding gene: 159184763

Following gene: 15888781

Centisome position: 51.02

GC content: 61.41

Gene sequence:

>1464_bases
ATGCACAACGAACATGATTTTTCTGCTGCTGGCGACCGCCTTTCAACCTTCCCCGCTTTCTTCCGGGTCGAGGGACGGCA
GGTCGCTGTTTTCGGAAATGGCGATGAAGCCTTCGCGAAGGTGAGGCTGCTCGCCAATACCAACGCGCATATCGTTGCCT
ATGCCGATCACCCGGAAGCGGATTTTGCGCGTTACATCAGCGAAAACAGTATCGATCACCGTGCTCTGGCCTTCGCCAGC
CCGTTGGTTGACGACGCCACCCTGGTGTTCGCCGCAACAGGCGATGAAGCGCTGGATCGCGAAATCGTCTCTGTAGCGCG
CACGAAGAAAATCCCCGCCAATGCGGTGGATCAGCCCGAATTCTGCGACTTCTTCACGCCTGCACTCGTTGCCCGCGCGC
CAGTTGCCATTGCCATTGGCACGGAAGGGGCAGGGCCGGTTCTTGCCCAGATGATCCGCGCGCGTATCGACCAGATGCTG
TCGCCGTCGCTCGGCAAGCTCGCACGTCTTGCGGTGCAATATCGTGATGCGGCCGAGGAGAACGTGCCGAAGGGCGCGTT
GCGGCGCAATTTCTGGCGCCGGTTCTTTTCGGGCGCGGTTGCGGATGCCGTTGCTCTCGGTGATACCTCTTCCGCCCGTC
ATGCCGCAGATCGCCTGCTGCAATCGCCGGAACGCAGCGAAGGTCGCGTCTGGCTTGTTGGCGCCGGTCCAGGTGCAGAA
GATCTGCTGACGCTGCGCGCGCAGCGCGTGTTGATGGAAGCCGACGTGATCGTCTACGACGCCCTGGTGCCGCAGGCGAT
TGTCGATATGGGCCGCCGCGATGCTGAAAGGCTCTCTGTCGGCAAGCGCAAGGGCTGCCACAGCAAGTCGCAAAATGATA
TCAACCGCCTGCTGGTGCGTCTAGGCCTGGAAGGAAAGCGAGTCGTGCGGCTCAAATCCGGTGATCCGCTGGTCTACGGC
CGCGCGGGCGAGGAAATGGCAGCGCTGCGCGAGGCTGGTGTCGCTTATGAAATCGTGCCGGGCATCACCTCCGCCTTTGC
CGCGGCTGCGGATTTCGAATTACCACTGACGCTGCGCGGTGTCGCCTCTTCGCTGATTTTCACCACTGGTCACGACCTGA
TGGGCGACGTTCTGCCTGATTGGGCACGACTTGCCGTCTCCGGGGCCACCATTGCCGTCTATATGGGCCGCAGTGTTGCC
GCTTCGGTGGCCAAGCGGCTGATTGATGCGGGACTTGGCGTCGAAACCACCGTCGCCGTCATCGAAAATGCCAGCCGCGC
GGATCGCCGCCTGCTGCACGGCACATTAAACGATTTGCCCGACCTCGAATATCGTGATGAACTGATCGGCCCGGTCATGG
TCATCATCGGCGATGCGGTGGCGGGCGCTAATTTCGACAGATCCGAGGCGCTTGCGCTTGCAGCCAAGCCGGCGAGCCTC
AAACGGGAGTATGTGAATGGTTGA

Upstream 100 bases:

>100_bases
CCTCCAAAACCGGGGTGTTCGAGAAATAAAAATCCAGGTGTCGAAGAGGCTTGAATTTCTGTTTCTTCAATCTTGTGCCG
CGATGCGGGATATATGTCCA

Downstream 100 bases:

>100_bases
CAAGGTTTTGACCGCCAATCGCCTGAGCGACGGTATTGCCGTCTGGCTTGACGCCAATGGCGAATGGACCGAGAATCTGC
AGGATGCAATCGTCGCTCGC

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MHNEHDFSAAGDRLSTFPAFFRVEGRQVAVFGNGDEAFAKVRLLANTNAHIVAYADHPEADFARYISENSIDHRALAFAS
PLVDDATLVFAATGDEALDREIVSVARTKKIPANAVDQPEFCDFFTPALVARAPVAIAIGTEGAGPVLAQMIRARIDQML
SPSLGKLARLAVQYRDAAEENVPKGALRRNFWRRFFSGAVADAVALGDTSSARHAADRLLQSPERSEGRVWLVGAGPGAE
DLLTLRAQRVLMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQNDINRLLVRLGLEGKRVVRLKSGDPLVYG
RAGEEMAALREAGVAYEIVPGITSAFAAAADFELPLTLRGVASSLIFTTGHDLMGDVLPDWARLAVSGATIAVYMGRSVA
ASVAKRLIDAGLGVETTVAVIENASRADRRLLHGTLNDLPDLEYRDELIGPVMVIIGDAVAGANFDRSEALALAAKPASL
KREYVNG

Sequences:

>Translated_487_residues
MHNEHDFSAAGDRLSTFPAFFRVEGRQVAVFGNGDEAFAKVRLLANTNAHIVAYADHPEADFARYISENSIDHRALAFAS
PLVDDATLVFAATGDEALDREIVSVARTKKIPANAVDQPEFCDFFTPALVARAPVAIAIGTEGAGPVLAQMIRARIDQML
SPSLGKLARLAVQYRDAAEENVPKGALRRNFWRRFFSGAVADAVALGDTSSARHAADRLLQSPERSEGRVWLVGAGPGAE
DLLTLRAQRVLMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQNDINRLLVRLGLEGKRVVRLKSGDPLVYG
RAGEEMAALREAGVAYEIVPGITSAFAAAADFELPLTLRGVASSLIFTTGHDLMGDVLPDWARLAVSGATIAVYMGRSVA
ASVAKRLIDAGLGVETTVAVIENASRADRRLLHGTLNDLPDLEYRDELIGPVMVIIGDAVAGANFDRSEALALAAKPASL
KREYVNG
>Mature_487_residues
MHNEHDFSAAGDRLSTFPAFFRVEGRQVAVFGNGDEAFAKVRLLANTNAHIVAYADHPEADFARYISENSIDHRALAFAS
PLVDDATLVFAATGDEALDREIVSVARTKKIPANAVDQPEFCDFFTPALVARAPVAIAIGTEGAGPVLAQMIRARIDQML
SPSLGKLARLAVQYRDAAEENVPKGALRRNFWRRFFSGAVADAVALGDTSSARHAADRLLQSPERSEGRVWLVGAGPGAE
DLLTLRAQRVLMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQNDINRLLVRLGLEGKRVVRLKSGDPLVYG
RAGEEMAALREAGVAYEIVPGITSAFAAAADFELPLTLRGVASSLIFTTGHDLMGDVLPDWARLAVSGATIAVYMGRSVA
ASVAKRLIDAGLGVETTVAVIENASRADRRLLHGTLNDLPDLEYRDELIGPVMVIIGDAVAGANFDRSEALALAAKPASL
KREYVNG

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=449, Percent_Identity=36.0801781737194, Blast_Score=289, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6322922, Length=261, Percent_Identity=31.0344827586207, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 52231; Mature: 52231

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNEHDFSAAGDRLSTFPAFFRVEGRQVAVFGNGDEAFAKVRLLANTNAHIVAYADHPEA
CCCCCCCCCCCCHHHHCCHHEEECCEEEEEEECCHHHHHEEEEEECCCCEEEEEECCCCH
DFARYISENSIDHRALAFASPLVDDATLVFAATGDEALDREIVSVARTKKIPANAVDQPE
HHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCH
FCDFFTPALVARAPVAIAIGTEGAGPVLAQMIRARIDQMLSPSLGKLARLAVQYRDAAEE
HHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NVPKGALRRNFWRRFFSGAVADAVALGDTSSARHAADRLLQSPERSEGRVWLVGAGPGAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
DLLTLRAQRVLMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQNDINRLLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCCCHHHHHHHHHH
LGLEGKRVVRLKSGDPLVYGRAGEEMAALREAGVAYEIVPGITSAFAAAADFELPLTLRG
HCCCCCEEEEEECCCCEEECCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEHHH
VASSLIFTTGHDLMGDVLPDWARLAVSGATIAVYMGRSVAASVAKRLIDAGLGVETTVAV
HHHHHHHHCCHHHHHHHCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHH
IENASRADRRLLHGTLNDLPDLEYRDELIGPVMVIIGDAVAGANFDRSEALALAAKPASL
HHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECHHHCCCCCHHHHEEEECCCHHH
KREYVNG
HHHHCCC
>Mature Secondary Structure
MHNEHDFSAAGDRLSTFPAFFRVEGRQVAVFGNGDEAFAKVRLLANTNAHIVAYADHPEA
CCCCCCCCCCCCHHHHCCHHEEECCEEEEEEECCHHHHHEEEEEECCCCEEEEEECCCCH
DFARYISENSIDHRALAFASPLVDDATLVFAATGDEALDREIVSVARTKKIPANAVDQPE
HHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCH
FCDFFTPALVARAPVAIAIGTEGAGPVLAQMIRARIDQMLSPSLGKLARLAVQYRDAAEE
HHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NVPKGALRRNFWRRFFSGAVADAVALGDTSSARHAADRLLQSPERSEGRVWLVGAGPGAE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHH
DLLTLRAQRVLMEADVIVYDALVPQAIVDMGRRDAERLSVGKRKGCHSKSQNDINRLLVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCCCHHHHHHHHHH
LGLEGKRVVRLKSGDPLVYGRAGEEMAALREAGVAYEIVPGITSAFAAAADFELPLTLRG
HCCCCCEEEEEECCCCEEECCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCEEHHH
VASSLIFTTGHDLMGDVLPDWARLAVSGATIAVYMGRSVAASVAKRLIDAGLGVETTVAV
HHHHHHHHCCHHHHHHHCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHH
IENASRADRRLLHGTLNDLPDLEYRDELIGPVMVIIGDAVAGANFDRSEALALAAKPASL
HHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEECHHHCCCCCHHHHEEEECCCHHH
KREYVNG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA