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The map label for this gene is gatB

Identifier: 159184713

GI number: 159184713

Start: 1312454

End: 1313959

Strand: Direct

Name: gatB

Synonym: Atu1324

Alternate gene names: 159184713

Gene position: 1312454-1313959 (Clockwise)

Preceding gene: 159184711

Following gene: 15888650

Centisome position: 46.19

GC content: 60.09

Gene sequence:

>1506_bases
ATGACCCTTGTAGACGTGCGCACCCCCGACCCGAAACGCTTCATTCCCGGCGCCACCGGCGATTGGGAAATCGTGATCGG
CCTCGAAGTCCATGCGCAGGTTCTCTCCAATTCCAAGCTGTTTTCCGGCGCGTCCACCACTTTCGGCAATGCGCCGAATT
CCAACGTCTCGCTGGTGGACGCCGCCATGCCCGGCATGCTGCCCGTTATCAACGAGGAATGCGTCAAACAGGCCGTGCGC
ACGGGCCTCGGCCTGAAGGCGAAGATCAACAACCGTTCGATCTTCGACCGCAAGAACTACTTTTATCCGGATTTGCCGCA
GGGCTACCAGATTTCGCAGTTCAAGGACCCGATCGTCGGTGAGGGCACCATCACCATTTCGCTCGGTCCGGACCGCCAGG
GCAACTTCGAGGATATCGAGATTGGCATCGAGCGCCTGCATCTGGAGCAGGACGCCGGCAAGTCGATGCACGACCAGCAT
CCGACCATGTCCTTCGTGGACCTGAACCGTTCGGGCGTGGCGCTGATGGAAATCGTCTCCAAGCCGGACATGCGCTCGTC
GGATGAGGCCAAGGGTTACCTCACCAAGCTGCGCTCCATCGTCCGTTACCTCGGCACCTGCGACGGCAATATGGACGAAG
GCTCGATGCGCGCCGACGTCAACGTCTCCGTGCGCCGCCCGGGCGAAGGTTTTGGCACGCGCTGCGAAATCAAGAACGTC
AACTCCATCCGCTTCGTCGGCCAGGCCATCGAATACGAGGCGCGCCGTCAGGTCGCCATTCTCGAAGATGGCGGTGTCAT
CGATCAGGAAACCCGCCTGTTCGATCCCGGCAAGGGCGAGACGCGGTCCATGCGCTCCAAGGAAGACGCGCATGACTATC
GCTATTTCCCGGATCCCGATCTGCTGCCGCTGGAATTCGATGATGCTTTTGTAGAAGCTCTGAAGGTCGATCTGCCAGAG
CTTCCCGATGACAAGAAGGCCCGTTTCGTGGCCGATCTCGGCTTGTCGGTCTATGACGCCTCGATTCTCGTGTCGGAAAA
GGCGATTGCCGATTATTATGAAGCCGTTGCCGCCGGTCGCGATGCGAAGACCGCTGCCAACTGGGTGATCAACGATCTTC
TCGGTGCGCTGAACAAGTTCGGCAAGGATATCGAGACGACTCCGGTTTCGCCCGACCAGCTGGGCGGCATCATCGATCTC
ATCAAGGCTGAGACCATTTCCGGCAAGATCGCCAAGGACCTGTTCGAAATCGTCTGGAACGAGGGCGGCAACCCGGCCGA
AATCGTCGAAGCCCGCGGCATGAAGCAGGTGACCGACACCGGCGCCATCGAAAAGGCTGTTGATGATATCATCGCCGCCA
ACCCGGATCAGGTCGAGAAGGTCAAGGCGAAGCCGACGCTTGCCGGCTGGTTCGTCGGTCAGGTGATGAAGGCGACGGGC
GGTAAGGCCAACCCGCAGGCAGTGCAGGCACTGGTCAAGGCCAAGCTCGGCATCGAGGACGAGTAA

Upstream 100 bases:

>100_bases
AAATCCGGCGTATGGCGCTACGGGGTGCAAGATGTCTTGCGCCCGCGCTGCATTTGTTCTACCCACCAAGGCCAAAGAAC
CCGTATATCGTGAGCTTCAG

Downstream 100 bases:

>100_bases
GCGGAGGCTGCCTCTTGCCGTAGAAGCATTGAGGCCGACAGCTCTCATCCGTCATCCTCGGGCTTGTCCCGAGGATCTAA
CGACCCACAAGGTCAAACAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTLVDVRTPDPKRFIPGATGDWEIVIGLEVHAQVLSNSKLFSGASTTFGNAPNSNVSLVDAAMPGMLPVINEECVKQAVR
TGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQFKDPIVGEGTITISLGPDRQGNFEDIEIGIERLHLEQDAGKSMHDQH
PTMSFVDLNRSGVALMEIVSKPDMRSSDEAKGYLTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
NSIRFVGQAIEYEARRQVAILEDGGVIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVEALKVDLPE
LPDDKKARFVADLGLSVYDASILVSEKAIADYYEAVAAGRDAKTAANWVINDLLGALNKFGKDIETTPVSPDQLGGIIDL
IKAETISGKIAKDLFEIVWNEGGNPAEIVEARGMKQVTDTGAIEKAVDDIIAANPDQVEKVKAKPTLAGWFVGQVMKATG
GKANPQAVQALVKAKLGIEDE

Sequences:

>Translated_501_residues
MTLVDVRTPDPKRFIPGATGDWEIVIGLEVHAQVLSNSKLFSGASTTFGNAPNSNVSLVDAAMPGMLPVINEECVKQAVR
TGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQFKDPIVGEGTITISLGPDRQGNFEDIEIGIERLHLEQDAGKSMHDQH
PTMSFVDLNRSGVALMEIVSKPDMRSSDEAKGYLTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
NSIRFVGQAIEYEARRQVAILEDGGVIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVEALKVDLPE
LPDDKKARFVADLGLSVYDASILVSEKAIADYYEAVAAGRDAKTAANWVINDLLGALNKFGKDIETTPVSPDQLGGIIDL
IKAETISGKIAKDLFEIVWNEGGNPAEIVEARGMKQVTDTGAIEKAVDDIIAANPDQVEKVKAKPTLAGWFVGQVMKATG
GKANPQAVQALVKAKLGIEDE
>Mature_500_residues
TLVDVRTPDPKRFIPGATGDWEIVIGLEVHAQVLSNSKLFSGASTTFGNAPNSNVSLVDAAMPGMLPVINEECVKQAVRT
GLGLKAKINNRSIFDRKNYFYPDLPQGYQISQFKDPIVGEGTITISLGPDRQGNFEDIEIGIERLHLEQDAGKSMHDQHP
TMSFVDLNRSGVALMEIVSKPDMRSSDEAKGYLTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNVN
SIRFVGQAIEYEARRQVAILEDGGVIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPDLLPLEFDDAFVEALKVDLPEL
PDDKKARFVADLGLSVYDASILVSEKAIADYYEAVAAGRDAKTAANWVINDLLGALNKFGKDIETTPVSPDQLGGIIDLI
KAETISGKIAKDLFEIVWNEGGNPAEIVEARGMKQVTDTGAIEKAVDDIIAANPDQVEKVKAKPTLAGWFVGQVMKATGG
KANPQAVQALVKAKLGIEDE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=40.2020202020202, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=39.9328859060403, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=534, Percent_Identity=31.8352059925094, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550808, Length=488, Percent_Identity=38.7295081967213, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_AGRT5 (Q8UFS3)

Other databases:

- EMBL:   AE007869
- PIR:   AD2739
- PIR:   B97520
- RefSeq:   NP_354330.2
- ProteinModelPortal:   Q8UFS3
- SMR:   Q8UFS3
- STRING:   Q8UFS3
- GeneID:   1133362
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1324
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q8UFS3
- ProtClustDB:   PRK05477
- BioCyc:   ATUM176299-1:ATU1324-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54692; Mature: 54560

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVDVRTPDPKRFIPGATGDWEIVIGLEVHAQVLSNSKLFSGASTTFGNAPNSNVSLVD
CEEEECCCCCCCCCCCCCCCCEEEEEEEEEHHHHHCCCCEECCCCCCCCCCCCCCCEEEE
AAMPGMLPVINEECVKQAVRTGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQFKDPIVG
HHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCHHCCCCCCC
EGTITISLGPDRQGNFEDIEIGIERLHLEQDAGKSMHDQHPTMSFVDLNRSGVALMEIVS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHC
KPDMRSSDEAKGYLTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFVGQAIEYEARRQVAILEDGGVIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
LLPLEFDDAFVEALKVDLPELPDDKKARFVADLGLSVYDASILVSEKAIADYYEAVAAGR
EEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHCCHHHEEHHHHHHHHHHHHHCCC
DAKTAANWVINDLLGALNKFGKDIETTPVSPDQLGGIIDLIKAETISGKIAKDLFEIVWN
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
EGGNPAEIVEARGMKQVTDTGAIEKAVDDIIAANPDQVEKVKAKPTLAGWFVGQVMKATG
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVQALVKAKLGIEDE
CCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLVDVRTPDPKRFIPGATGDWEIVIGLEVHAQVLSNSKLFSGASTTFGNAPNSNVSLVD
EEEECCCCCCCCCCCCCCCCEEEEEEEEEHHHHHCCCCEECCCCCCCCCCCCCCCEEEE
AAMPGMLPVINEECVKQAVRTGLGLKAKINNRSIFDRKNYFYPDLPQGYQISQFKDPIVG
HHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCHHCCCCCCC
EGTITISLGPDRQGNFEDIEIGIERLHLEQDAGKSMHDQHPTMSFVDLNRSGVALMEIVS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHC
KPDMRSSDEAKGYLTKLRSIVRYLGTCDGNMDEGSMRADVNVSVRRPGEGFGTRCEIKNV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCC
NSIRFVGQAIEYEARRQVAILEDGGVIDQETRLFDPGKGETRSMRSKEDAHDYRYFPDPD
CHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHCCCCHHHCCCCCCCCC
LLPLEFDDAFVEALKVDLPELPDDKKARFVADLGLSVYDASILVSEKAIADYYEAVAAGR
EEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHCCHHHEEHHHHHHHHHHHHHCCC
DAKTAANWVINDLLGALNKFGKDIETTPVSPDQLGGIIDLIKAETISGKIAKDLFEIVWN
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
EGGNPAEIVEARGMKQVTDTGAIEKAVDDIIAANPDQVEKVKAKPTLAGWFVGQVMKATG
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCC
GKANPQAVQALVKAKLGIEDE
CCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194