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The map label for this gene is gatA

Identifier: 159184709

GI number: 159184709

Start: 1308910

End: 1310391

Strand: Direct

Name: gatA

Synonym: Atu1319

Alternate gene names: 159184709

Gene position: 1308910-1310391 (Clockwise)

Preceding gene: 159184708

Following gene: 159184710

Centisome position: 46.06

GC content: 62.28

Gene sequence:

>1482_bases
ATGACCGACCTGACGAGCCTGACCATTGCCGAAGCCCGCGAAAAGCTGAAGGCGAAAGATTTTTCCGCCCTTGAGCTGAC
CGATGCCTATCTCTCCGCCATCGACGCGGCGAACGGCGCGCTGAACGCCTATGTGGCGCTGACGCCTGAAAAGGCGCGCG
ACATGGCAAAGGCCTCCGACGAACGCATCGCCTCAGGCTCGGCTGGCGAGCTGGAAGGTGTTCCGCTCGGCGTGAAGGAC
CTGTTCGCCACCCGCGATGTGCACACGCAGGCATGTTCGCATGTTCTTGACGGCTTCAAGCCGAAATACGAATCCACCGT
CACCCAGAACCTCTGGGATCAGGGCGCCGTCATGCTCGGCAAGCTCAACATGGACGAATTCGCCATGGGCTCGTCCAATG
AAAGCTCCTGGTACGGCCCGGTCATCAACCCCTGGCGCGCCAACGGTTCCGAGCAGAAGCTCGTTCCCGGTGGTTCCTCC
GGCGGTTCGGCCGCTGCCGTTGCCGCGCATCTCTGCGCCGGCGCCACCGCAACCGACACAGGCGGCTCCATCCGCCAGCC
CGCGGCCTTCACCGGTACTGTCGGCATCAAGCCCACCTATGGCCGCTGCTCGCGCTTCGGCATCGTCGCTTATGCTTCCT
CGCTCGATCAGGCTGGCCCGATTGCCCGTGATGTGCGTGACGCGGCGATCCTGCTGAAGACCATGGCAAGTGTCGATGCC
AAGGATACGACCTCCGTTGATCTGCCGGTGCCGGATTACGAAAAGGCCATCGGCCAGTCGCTGAAGGGCCTGAAGATCGG
CATTCCCCGCGAGTACCGCGTCGATGGCATGCCGGAAGAGATCGAAAAGCTCTGGGCCAAGGGCGTCGAGTGGCTGCGCG
ATGCGGGTGCCGAGGTGGTCGATATCTCGCTGCCGCACACCAAATACGCGCTGCCCGCCTATTACATCGTCGCACCGGCG
GAAGCCTCCTCCAATCTCGCACGTTATGATGGCGTGCGCTACGGCCTGCGCGTCGACGGCAAGGACATTGCTGACATGTA
CGAAAAGAGCCGTGCGGCCGGTTTCGGCAAGGAAGTGCAGCGCCGCATCATGGTCGGCACATACGTGCTTTCGGCCGGTT
ATTACGATGCCTATTACCTCAAGGCGCAGAAGGTCCGCACCCTCATCAAGCGTGACTTCGAAAACGTCTTCCATGAAGGC
GTCGATGCCATCCTGGCGCCGATCACACCGTCTTCCGCCTTCGCAGTCGGTGACGAGGAACTCGCTTCCGATCCGGTGAA
GATGTATCTCCAGGACGTCTTCACCATCACCGTCAACATGGCTGGCCTTCCCGGCCTTTCGGTCCCGGCCGGTCTCGACG
GCAAGGGGCTGCCGCTCGGCCTGCAGCTCATCGGCAAGCCTTTCGAGGAAGAAACGCTGTTCAAGACGGCCCATGCCATC
GAACAGGCCGCCGGCAAGTTCACCCCTGCCAAGTGGTGGTAA

Upstream 100 bases:

>100_bases
CGACCGACCGGGACTTCTTCATGGTCCCGAAAGTGGTCGAATAATCCGACCGCCGTTTGCCGCCCGATTTTTCCGAGATT
TTCGAAAGCCGTTGCCGACC

Downstream 100 bases:

>100_bases
GCGACCTCAAGCTCAGAGACATGAACGAAGCCGATCGCCATGCGGTCGGCTTTGTCGGTTTTGCTGCCTGGCGTGCGGGT
GACGCCTTTGACGCCTCCTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTDLTSLTIAEAREKLKAKDFSALELTDAYLSAIDAANGALNAYVALTPEKARDMAKASDERIASGSAGELEGVPLGVKD
LFATRDVHTQACSHVLDGFKPKYESTVTQNLWDQGAVMLGKLNMDEFAMGSSNESSWYGPVINPWRANGSEQKLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRFGIVAYASSLDQAGPIARDVRDAAILLKTMASVDA
KDTTSVDLPVPDYEKAIGQSLKGLKIGIPREYRVDGMPEEIEKLWAKGVEWLRDAGAEVVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVDGKDIADMYEKSRAAGFGKEVQRRIMVGTYVLSAGYYDAYYLKAQKVRTLIKRDFENVFHEG
VDAILAPITPSSAFAVGDEELASDPVKMYLQDVFTITVNMAGLPGLSVPAGLDGKGLPLGLQLIGKPFEEETLFKTAHAI
EQAAGKFTPAKWW

Sequences:

>Translated_493_residues
MTDLTSLTIAEAREKLKAKDFSALELTDAYLSAIDAANGALNAYVALTPEKARDMAKASDERIASGSAGELEGVPLGVKD
LFATRDVHTQACSHVLDGFKPKYESTVTQNLWDQGAVMLGKLNMDEFAMGSSNESSWYGPVINPWRANGSEQKLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRFGIVAYASSLDQAGPIARDVRDAAILLKTMASVDA
KDTTSVDLPVPDYEKAIGQSLKGLKIGIPREYRVDGMPEEIEKLWAKGVEWLRDAGAEVVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVDGKDIADMYEKSRAAGFGKEVQRRIMVGTYVLSAGYYDAYYLKAQKVRTLIKRDFENVFHEG
VDAILAPITPSSAFAVGDEELASDPVKMYLQDVFTITVNMAGLPGLSVPAGLDGKGLPLGLQLIGKPFEEETLFKTAHAI
EQAAGKFTPAKWW
>Mature_492_residues
TDLTSLTIAEAREKLKAKDFSALELTDAYLSAIDAANGALNAYVALTPEKARDMAKASDERIASGSAGELEGVPLGVKDL
FATRDVHTQACSHVLDGFKPKYESTVTQNLWDQGAVMLGKLNMDEFAMGSSNESSWYGPVINPWRANGSEQKLVPGGSSG
GSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRFGIVAYASSLDQAGPIARDVRDAAILLKTMASVDAK
DTTSVDLPVPDYEKAIGQSLKGLKIGIPREYRVDGMPEEIEKLWAKGVEWLRDAGAEVVDISLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVDGKDIADMYEKSRAAGFGKEVQRRIMVGTYVLSAGYYDAYYLKAQKVRTLIKRDFENVFHEGV
DAILAPITPSSAFAVGDEELASDPVKMYLQDVFTITVNMAGLPGLSVPAGLDGKGLPLGLQLIGKPFEEETLFKTAHAIE
QAAGKFTPAKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=495, Percent_Identity=40.8080808080808, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=25.4980079681275, Blast_Score=107, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=36.7945823927765, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=507, Percent_Identity=25.2465483234714, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538252, Length=269, Percent_Identity=24.5353159851301, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=226, Percent_Identity=28.3185840707965, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=318, Percent_Identity=24.5283018867925, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=206, Percent_Identity=32.0388349514563, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=206, Percent_Identity=32.0388349514563, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=431, Percent_Identity=36.6589327146172, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319685, Length=410, Percent_Identity=27.8048780487805, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=242, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648113, Length=451, Percent_Identity=41.4634146341463, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=23.7816764132554, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161078093, Length=523, Percent_Identity=25.0478011472275, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=24.8015873015873, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=23.1707317073171, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=23.1707317073171, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=23.1707317073171, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=23.1707317073171, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI45550774, Length=249, Percent_Identity=28.5140562248996, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648435, Length=249, Percent_Identity=28.5140562248996, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648437, Length=249, Percent_Identity=28.5140562248996, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_AGRT5 (Q8UFS8)

Other databases:

- EMBL:   AE007869
- PIR:   AG2738
- PIR:   E97519
- RefSeq:   NP_354325.2
- ProteinModelPortal:   Q8UFS8
- SMR:   Q8UFS8
- STRING:   Q8UFS8
- GeneID:   1133357
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1319
- NMPDR:   fig|176299.3.peg.1652
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   ATUM176299-1:ATU1319-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52768; Mature: 52637

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 159-159 ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLTSLTIAEAREKLKAKDFSALELTDAYLSAIDAANGALNAYVALTPEKARDMAKASD
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHH
ERIASGSAGELEGVPLGVKDLFATRDVHTQACSHVLDGFKPKYESTVTQNLWDQGAVMLG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEE
KLNMDEFAMGSSNESSWYGPVINPWRANGSEQKLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRCSRFGIVAYASSLDQAGPIARDVRDAAILLKTMASVDA
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
KDTTSVDLPVPDYEKAIGQSLKGLKIGIPREYRVDGMPEEIEKLWAKGVEWLRDAGAEVV
CCCCEEECCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKDIADMYEKSRAAGFGKEVQ
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
RRIMVGTYVLSAGYYDAYYLKAQKVRTLIKRDFENVFHEGVDAILAPITPSSAFAVGDEE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCCEECCCHH
LASDPVKMYLQDVFTITVNMAGLPGLSVPAGLDGKGLPLGLQLIGKPFEEETLFKTAHAI
HHCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHH
EQAAGKFTPAKWW
HHHHCCCCCCCCC
>Mature Secondary Structure 
TDLTSLTIAEAREKLKAKDFSALELTDAYLSAIDAANGALNAYVALTPEKARDMAKASD
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHH
ERIASGSAGELEGVPLGVKDLFATRDVHTQACSHVLDGFKPKYESTVTQNLWDQGAVMLG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEE
KLNMDEFAMGSSNESSWYGPVINPWRANGSEQKLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRCSRFGIVAYASSLDQAGPIARDVRDAAILLKTMASVDA
CCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
KDTTSVDLPVPDYEKAIGQSLKGLKIGIPREYRVDGMPEEIEKLWAKGVEWLRDAGAEVV
CCCCEEECCCCCHHHHHHCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKDIADMYEKSRAAGFGKEVQ
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
RRIMVGTYVLSAGYYDAYYLKAQKVRTLIKRDFENVFHEGVDAILAPITPSSAFAVGDEE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCCCCEECCCHH
LASDPVKMYLQDVFTITVNMAGLPGLSVPAGLDGKGLPLGLQLIGKPFEEETLFKTAHAI
HHCCHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHH
EQAAGKFTPAKWW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194