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The map label for this gene is pleD [H]

Identifier: 159184666

GI number: 159184666

Start: 1200490

End: 1201755

Strand: Direct

Name: pleD [H]

Synonym: Atu1207

Alternate gene names: 159184666

Gene position: 1200490-1201755 (Clockwise)

Preceding gene: 159184665

Following gene: 15888545

Centisome position: 42.25

GC content: 58.77

Gene sequence:

>1266_bases
ATGCAATGGGCAAATCTGGCCCTCTTTCTGACGGAGGCCATCGTCTATTTTTCGGTAATGACGGCATTCCTTCATTACCG
GCATATTCTCGGCATCGGTGTTTTTCTCACCGCGCTGGGCGTCATGCATTTCCTTGAAACCTATCTGGCCGCCGTTTTTT
ACGTGCAGCTGCCCTTTGGCGTGGCATCGCCCGGCTCCTCGATCCTTTTTGCCGGCAAGCTGATGATGATCCTCATGCTC
TATATGCGCGAGGATGCCGCCGTGGTGCGACAGCCGATATACGGCCTGTTTCTCGGCAATATATTGACCGTCATCATGGC
GCAGATCATCCGTTTTCACCAAACGGTGGCGATCGTTCCCGGCCAGTCGGTCAGTACCGGCTTTCTCGATGAGATGGGCA
TCCTGATGGTCTGGGGAACCAGCCTTCTCTACATCGACGCCATCGCCATCATCCTGTTTTACGAGAAGCTCGGGCGTTAT
CTGCAGCGCCATATCGTGCTGCGCTTCGCGATCTGCGGCGTCGTTATCCTGAGCTTCGATCAGGCGGGTTTTTATGGGGC
GTTGCGGTTGTTGTTCAACGCACCTGTTGATGTCTTTTACGATGGCTGGAAGGCGAAGATGGCGGCGGTCGGCATCTATT
CGCTGCTCTTTGCCACCTATCTCTGGCTGACAGCGGCCAAGGGCCGCTTTCTGACACGGCGCAGCGTTGCCGATGTCTTC
AACGACTTGACCTTCCGGGAACGCTATGAGGAGCTTTTGTCCCGCTCGGGACGCGATATGCTGACCGGCGTATCGGACCG
CGCCCGCATGGAACTTGATGCGCCCTCCCTCGTCGTGGAGTGCCTGGAGAAGCGGCGGCCGGTCAGCGTGCTGATCGTCG
ATATCGACCATTTCAAGACCGTCAATGACACGTTCGGGCACCTGCAGGGCGATGAGATCTTGCGGGAATTCGCCGCCGTA
CTGAAACGCGCCGTGCAGCCGCTGGGACATCTTTACCGTTTCGGCGGCGAGGAATTCGTCGCGCTCCTGCCGGCGATGAC
GCATGAAACAGCGCTTGCATTTTCGGCCAGTCTCCGGGTGGCGGTCCTCGCGGAGCTGCAACGGCCGGACGGTTCGCCGC
TGACTGTCAGTATCGGCGTCGCTACCGCCTTCGAGGATGGGCAGCTTTTCCGCGCGCTTTTATCGGAGGCCGATGCGTGG
CTCTACGCGGCAAAAAATAACGGCCGGGACCAGGTCCACGGCCGTCATGGCATGTGGGTCGGCTGA

Upstream 100 bases:

>100_bases
TTAGAGCAATTCCGGTGAACCGCGTTCACAGCAATTGCTCTAGAGGGCGTGGAGTCGCGTCAATCAACCAGCGCGATATG
GATCGATAGGGCGTGTGCCG

Downstream 100 bases:

>100_bases
TGCCGGCGATAGCCCGCATCAGCCTTCATTCAACGGGAATCAGGCGGCCTGCGCTAGTTCTTCGACCTTGGCTTCGGCAG
CAGCAACGGCCTTTTCGACG

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MQWANLALFLTEAIVYFSVMTAFLHYRHILGIGVFLTALGVMHFLETYLAAVFYVQLPFGVASPGSSILFAGKLMMILML
YMREDAAVVRQPIYGLFLGNILTVIMAQIIRFHQTVAIVPGQSVSTGFLDEMGILMVWGTSLLYIDAIAIILFYEKLGRY
LQRHIVLRFAICGVVILSFDQAGFYGALRLLFNAPVDVFYDGWKAKMAAVGIYSLLFATYLWLTAAKGRFLTRRSVADVF
NDLTFRERYEELLSRSGRDMLTGVSDRARMELDAPSLVVECLEKRRPVSVLIVDIDHFKTVNDTFGHLQGDEILREFAAV
LKRAVQPLGHLYRFGGEEFVALLPAMTHETALAFSASLRVAVLAELQRPDGSPLTVSIGVATAFEDGQLFRALLSEADAW
LYAAKNNGRDQVHGRHGMWVG

Sequences:

>Translated_421_residues
MQWANLALFLTEAIVYFSVMTAFLHYRHILGIGVFLTALGVMHFLETYLAAVFYVQLPFGVASPGSSILFAGKLMMILML
YMREDAAVVRQPIYGLFLGNILTVIMAQIIRFHQTVAIVPGQSVSTGFLDEMGILMVWGTSLLYIDAIAIILFYEKLGRY
LQRHIVLRFAICGVVILSFDQAGFYGALRLLFNAPVDVFYDGWKAKMAAVGIYSLLFATYLWLTAAKGRFLTRRSVADVF
NDLTFRERYEELLSRSGRDMLTGVSDRARMELDAPSLVVECLEKRRPVSVLIVDIDHFKTVNDTFGHLQGDEILREFAAV
LKRAVQPLGHLYRFGGEEFVALLPAMTHETALAFSASLRVAVLAELQRPDGSPLTVSIGVATAFEDGQLFRALLSEADAW
LYAAKNNGRDQVHGRHGMWVG
>Mature_421_residues
MQWANLALFLTEAIVYFSVMTAFLHYRHILGIGVFLTALGVMHFLETYLAAVFYVQLPFGVASPGSSILFAGKLMMILML
YMREDAAVVRQPIYGLFLGNILTVIMAQIIRFHQTVAIVPGQSVSTGFLDEMGILMVWGTSLLYIDAIAIILFYEKLGRY
LQRHIVLRFAICGVVILSFDQAGFYGALRLLFNAPVDVFYDGWKAKMAAVGIYSLLFATYLWLTAAKGRFLTRRSVADVF
NDLTFRERYEELLSRSGRDMLTGVSDRARMELDAPSLVVECLEKRRPVSVLIVDIDHFKTVNDTFGHLQGDEILREFAAV
LKRAVQPLGHLYRFGGEEFVALLPAMTHETALAFSASLRVAVLAELQRPDGSPLTVSIGVATAFEDGQLFRALLSEADAW
LYAAKNNGRDQVHGRHGMWVG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=35.4285714285714, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=214, Percent_Identity=31.7757009345794, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=31.3953488372093, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=32.9113924050633, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=32.9268292682927, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=41.4634146341463, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787541, Length=215, Percent_Identity=30.2325581395349, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=29.1208791208791, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=29.3413173652695, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47031; Mature: 47031

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA