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The map label for this gene is gsiB [H]

Identifier: 159184662

GI number: 159184662

Start: 1194886

End: 1196379

Strand: Direct

Name: gsiB [H]

Synonym: Atu1202

Alternate gene names: 159184662

Gene position: 1194886-1196379 (Clockwise)

Preceding gene: 15888537

Following gene: 159184664

Centisome position: 42.05

GC content: 56.16

Gene sequence:

>1494_bases
ATGATAAAGCACGCACTTTTAACCACCTGCGCCATTGCCGGCGCTTTTGGGGCTAATGCCGCAAATGCCGGCACCGTTAC
TTATGACGATAACTACGCCGTGAAAGCTGCGTGGCAGATGGCGTCAGATGACTCTACTCTCGGCATGCGCGTGGGGTGTT
ACGAAGGCCTGGTCCGAATTGGCTTCGACATGAGGCTGGAGGCATCACTTGCGGAATCCTGGAAGCAAACGGAACCGAAG
GTGTGGGAGTTCAAGCTGCGCAAGGGCGTTAAATTCCAGAACGGCGAAGACTTTAATGCCGCTGCGGCCGTTAATGCCCT
CACCCTTCTTCTGAAGGCATCCGTTCCTGCCCGCGCCTTTTCGCCGAAACAGATCGCTGGGGTGGAAGCCGTTGGCGATG
ATGTTGTGAAGGTGACGACGATCGAACCATCGGTGCTGTTGCCCGCGCAGTTGGCAAGCCCCGCCACCGCCATCCTTGCG
CCATCGGCCTATAAAGACGGCAAGATCAATCCGATCGGTACCTGCACCGGACCGTTCGAGATCACGGAGGTCGATCCCAA
CCAATATATGGTCATGAAGGCCAACCACGGATACTGGGGTGGCATGCCGAAACTGGACGGTGGTCGCGTCAATTTCGTTC
CCGACGCCGACACTCGCGCCACCCAGATAAGGACGGGCGAAGTGCAGATTTCTCGTCTGGTGCCCCCCTGGTCGATCAAA
ACCATCGAATCCACAAAGGGTGTCAAGGTCGCCGCTATTTCAGTGCCGCGCATTACAGAGTTGCTGCTGAACAATTCCAG
GCCACCGTTCAAAGATAAAAAGGTTCGTGAGGCCGTGCGTGCGGCAATCGACGCCGCCGGCATCGCCAGCAGCATTTACG
AAGAGAAGGTCAAAAGCGCGAGCATGCCGTTTGCGCCTGGGCAGCCCTGGGCGGCGGAAGAACCCACATCGACCTATGAC
GTGGCAAAGGCAAAGTCTCTCCTCAAGGAAGCCGGCATCGCGCCGGGCAGCCTGAAAGTCACCATTCTTGCTTACACGAT
GAAGACCGAATTGAAAGATGTGGCCGCGATTATTCAGGCGCAGTTGGAGGAGATCGGTATCAAGGTCGATGTCCGCGTCG
CAGAATATAGCGCGATCGAGCCCGACCTTCTCGCCGGCAACTTCGACATGGTGTTGCTCTCCCGTGGTTACGCCACGGAC
GTCATCGAGCCGGCTGGATATCTCAACGCCGACTATTCCTGCACCGGAACGTACAACATCTCGCATTATTGCAATGAAGA
AACGGACAAGTTGATCAAGGCCGCCTATGCCGAAGTCGAACCAGCGAAGCGTTACGCGATCTACGGTCAGATAGCGAAGA
AGATCTATGACGATGCCGTCTCGGTCTTCCTCGTCCACGAGACCGTGTTCGATGCCTATTCCGAGAAACTCGAAAATTTC
CGACCGCACCCGATCAACTATTTCGTGCTGACGAAGGAACTGTCGATCAAGTAG

Upstream 100 bases:

>100_bases
TCCGCCAATAGATATTTTCGCCGTTTATAATAGAAGGAATAATATACTTTATAGTAATTGAATTGTGAGCGTCATCATCA
AAAATAAGGGGAATTATCCA

Downstream 100 bases:

>100_bases
ATCGACGTTATGAAAAAGACCCGGCTCCCAGGAGCCGGGTTCCACAAAGACGTAGTCCGGACTTTGAAAACCCGATCAAT
GTTCCCCGCTCTTGCACGCG

Product: ABC transporter, substrate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MIKHALLTTCAIAGAFGANAANAGTVTYDDNYAVKAAWQMASDDSTLGMRVGCYEGLVRIGFDMRLEASLAESWKQTEPK
VWEFKLRKGVKFQNGEDFNAAAAVNALTLLLKASVPARAFSPKQIAGVEAVGDDVVKVTTIEPSVLLPAQLASPATAILA
PSAYKDGKINPIGTCTGPFEITEVDPNQYMVMKANHGYWGGMPKLDGGRVNFVPDADTRATQIRTGEVQISRLVPPWSIK
TIESTKGVKVAAISVPRITELLLNNSRPPFKDKKVREAVRAAIDAAGIASSIYEEKVKSASMPFAPGQPWAAEEPTSTYD
VAKAKSLLKEAGIAPGSLKVTILAYTMKTELKDVAAIIQAQLEEIGIKVDVRVAEYSAIEPDLLAGNFDMVLLSRGYATD
VIEPAGYLNADYSCTGTYNISHYCNEETDKLIKAAYAEVEPAKRYAIYGQIAKKIYDDAVSVFLVHETVFDAYSEKLENF
RPHPINYFVLTKELSIK

Sequences:

>Translated_497_residues
MIKHALLTTCAIAGAFGANAANAGTVTYDDNYAVKAAWQMASDDSTLGMRVGCYEGLVRIGFDMRLEASLAESWKQTEPK
VWEFKLRKGVKFQNGEDFNAAAAVNALTLLLKASVPARAFSPKQIAGVEAVGDDVVKVTTIEPSVLLPAQLASPATAILA
PSAYKDGKINPIGTCTGPFEITEVDPNQYMVMKANHGYWGGMPKLDGGRVNFVPDADTRATQIRTGEVQISRLVPPWSIK
TIESTKGVKVAAISVPRITELLLNNSRPPFKDKKVREAVRAAIDAAGIASSIYEEKVKSASMPFAPGQPWAAEEPTSTYD
VAKAKSLLKEAGIAPGSLKVTILAYTMKTELKDVAAIIQAQLEEIGIKVDVRVAEYSAIEPDLLAGNFDMVLLSRGYATD
VIEPAGYLNADYSCTGTYNISHYCNEETDKLIKAAYAEVEPAKRYAIYGQIAKKIYDDAVSVFLVHETVFDAYSEKLENF
RPHPINYFVLTKELSIK
>Mature_497_residues
MIKHALLTTCAIAGAFGANAANAGTVTYDDNYAVKAAWQMASDDSTLGMRVGCYEGLVRIGFDMRLEASLAESWKQTEPK
VWEFKLRKGVKFQNGEDFNAAAAVNALTLLLKASVPARAFSPKQIAGVEAVGDDVVKVTTIEPSVLLPAQLASPATAILA
PSAYKDGKINPIGTCTGPFEITEVDPNQYMVMKANHGYWGGMPKLDGGRVNFVPDADTRATQIRTGEVQISRLVPPWSIK
TIESTKGVKVAAISVPRITELLLNNSRPPFKDKKVREAVRAAIDAAGIASSIYEEKVKSASMPFAPGQPWAAEEPTSTYD
VAKAKSLLKEAGIAPGSLKVTILAYTMKTELKDVAAIIQAQLEEIGIKVDVRVAEYSAIEPDLLAGNFDMVLLSRGYATD
VIEPAGYLNADYSCTGTYNISHYCNEETDKLIKAAYAEVEPAKRYAIYGQIAKKIYDDAVSVFLVHETVFDAYSEKLENF
RPHPINYFVLTKELSIK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=507, Percent_Identity=28.2051282051282, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789966, Length=454, Percent_Identity=25.9911894273128, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787762, Length=462, Percent_Identity=25.5411255411255, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789887, Length=437, Percent_Identity=29.2906178489703, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787551, Length=434, Percent_Identity=23.0414746543779, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787495, Length=449, Percent_Identity=24.7216035634744, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI87081878, Length=443, Percent_Identity=24.1534988713318, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789397, Length=494, Percent_Identity=23.8866396761134, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 53923; Mature: 53923

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKHALLTTCAIAGAFGANAANAGTVTYDDNYAVKAAWQMASDDSTLGMRVGCYEGLVRI
CCCHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEEECCCCCCCEEEEHHHHHHHHC
GFDMRLEASLAESWKQTEPKVWEFKLRKGVKFQNGEDFNAAAAVNALTLLLKASVPARAF
CCCEEEHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SPKQIAGVEAVGDDVVKVTTIEPSVLLPAQLASPATAILAPSAYKDGKINPIGTCTGPFE
CCHHHCCCHHCCCCEEEEEEECCCEECCHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEE
ITEVDPNQYMVMKANHGYWGGMPKLDGGRVNFVPDADTRATQIRTGEVQISRLVPPWSIK
EEEECCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCEEEEECCCCCCEE
TIESTKGVKVAAISVPRITELLLNNSRPPFKDKKVREAVRAAIDAAGIASSIYEEKVKSA
EECCCCCEEEEEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SMPFAPGQPWAAEEPTSTYDVAKAKSLLKEAGIAPGSLKVTILAYTMKTELKDVAAIIQA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHHH
QLEEIGIKVDVRVAEYSAIEPDLLAGNFDMVLLSRGYATDVIEPAGYLNADYSCTGTYNI
HHHHCCCEEEEEEECCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECH
SHYCNEETDKLIKAAYAEVEPAKRYAIYGQIAKKIYDDAVSVFLVHETVFDAYSEKLENF
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCC
RPHPINYFVLTKELSIK
CCCCEEEEEEEEEECCC
>Mature Secondary Structure
MIKHALLTTCAIAGAFGANAANAGTVTYDDNYAVKAAWQMASDDSTLGMRVGCYEGLVRI
CCCHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEEECCCCCCCEEEEHHHHHHHHC
GFDMRLEASLAESWKQTEPKVWEFKLRKGVKFQNGEDFNAAAAVNALTLLLKASVPARAF
CCCEEEHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
SPKQIAGVEAVGDDVVKVTTIEPSVLLPAQLASPATAILAPSAYKDGKINPIGTCTGPFE
CCHHHCCCHHCCCCEEEEEEECCCEECCHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEE
ITEVDPNQYMVMKANHGYWGGMPKLDGGRVNFVPDADTRATQIRTGEVQISRLVPPWSIK
EEEECCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCEEEEECCCCCCEE
TIESTKGVKVAAISVPRITELLLNNSRPPFKDKKVREAVRAAIDAAGIASSIYEEKVKSA
EECCCCCEEEEEEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SMPFAPGQPWAAEEPTSTYDVAKAKSLLKEAGIAPGSLKVTILAYTMKTELKDVAAIIQA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHHH
QLEEIGIKVDVRVAEYSAIEPDLLAGNFDMVLLSRGYATDVIEPAGYLNADYSCTGTYNI
HHHHCCCEEEEEEECCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECH
SHYCNEETDKLIKAAYAEVEPAKRYAIYGQIAKKIYDDAVSVFLVHETVFDAYSEKLENF
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCC
RPHPINYFVLTKELSIK
CCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA