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The map label for this gene is yoaE [H]

Identifier: 159184631

GI number: 159184631

Start: 1124885

End: 1127041

Strand: Direct

Name: yoaE [H]

Synonym: Atu1136

Alternate gene names: 159184631

Gene position: 1124885-1127041 (Clockwise)

Preceding gene: 15888471

Following gene: 159184632

Centisome position: 39.59

GC content: 62.17

Gene sequence:

>2157_bases
ATGAACGTTGCGACCCCGATTTCCGCTGAAAAAGCCGCCTCCAAGGCCGAAGTCCGTATTGGCCATACGGTCTGCCCGCA
TGACTGTCCGAGCGCCTGCGCGCTGGAGGTCGATATCAATGCCGATGGCCGCATCGGCCGTGTGCGCGGCGCCAATGCCA
ATACATATACCGCCGGCGTCATCTGCGCCAAGGTGGCGCGTTATTCCGAGCGCATCTACCATCCCGGCCGCCTGCTGACG
CCGAAGCGTCGCAAGGGCGCAAAAGGGGCGGGCGACTGGCAGGAGATTTCCTGGGAAGCGGCGCTGGACGAGGTGGCAGA
CGCTTTCGTCAAGGCGGAAGCCCGGCACGGTGCCGAGGCCATCTGGCCCTATTTTTACGCCGGCACGATGGGGCAGGTGC
AGCGGGACTCCATTGAACGTCTGCGCCACGCCAAAAAATACTCCGGCTTCTTCGGTTCCATCTGCACCAACATGGCCTGG
ACGGGCTATGTGATGGGCACCGGCGCGCTGCGCGGCCCCGATCCGCGCGAGATCGGCAAGGCCGATGTCGTCGTCATCTG
GGGTACCAATGCGGTGGCCACCCAGGTCAACGTCATGACCCATGCGGTGAAAGCCCGCAAGGAGCGTGGCGCAAAGATCG
TCGTTGTCGATATCTACGACAATCCGACCATGAAACAGGCGGATATGCGCATCGTTGTTCGCCCTGGCACGGATGCGGCA
CTGGCCTGCGCCGTCATGCACATCGCCTTCCGCGACGGTTATGCCGATCGCAATTACATGGCCCGCTTCGCCGACGATCC
GGCGGGGCTCGAAGCGCATCTGAAGTCAAAGACGCCGCAATGGGCATCCGAGATCACCGGTCTTTCCGTGGAAGAGATCG
AGGCTTTCGCGCGGCTCGTCGGCACGACGAAAAAGACCTTCTTCCGGCTGGGTTACGGTTTTGCCCGCCAGCGCAATGGC
GCGGTCGCGATGCATGCGGCGCTTTCCATCGCCACGGTTCTCGGCTCCTGGCAATATGAGGGCGGCGGCGCATTCCACAA
TAATGGCGATATCTTCCGGCTCAACAAGGCGGAGCTGATGGGCACAGCCTATGCCGACCCCGACGTGCGCCAGCTCGACC
AGTCGCAGATCGGCCGCGTGCTGACGGGGGACGCGGAAGCGTTGCGCCATGGCGGCCCGGTGACGGCGCTGCTCATCCAG
AACACCAATCCCGTCAATGTCGCGCCTGAACAGCGGCTGGTGAAACAGGGCTTCCTGCGCGACGATCTCTTCGTTGCGGT
GCACGAGCAGTTCATGACGGAGACGGCCGAGGTGGCCGATATCGTTATGCCCGCTACCATGTTCCTCGAACATGACGATA
TCTATCGCGCCGGCGGCCAAAACCACATTCTGCTCGGCCCCAAACTGGTGGAGCCGCCGGAAACGGTGCGCACCAACCTC
TTCGTCATCGAGGAACTGGCAAAGCGCCTCGGTGTCGACCATATGCCCGGCTTCGGCCTCTCTGCCCGCGAGCATGTCGA
CCGTATGCTCAAAGTCAGCGGCTGGGGCGATTTCGAAAGCCTGGCGGAAGAGAAATGGATCGATGCGCAGCCGCCTTTCG
AGATGGCGCATTATCTCGAGGGTTTTGGTTATGGCGACGGAAAGTTCCGCTTCAGCCCCGACTGGGCAACCGGACCGTCT
CCGAACAAGCCGCCGAAGAACATCGGCGTCATGGGGCCGGTTTCGGAGTTTCCGAAATTCCCGGATCAGGTCGATGTCAT
CGAGGTTGCGGATGCGGACCATCCCTTCCGGCTGGCGACGTCACCGGCACGCAACTTCCTGAACTCCACCTTCGCCGAAA
CCAAAACCTCCGTGCAGAAGGAAGGCCGTCCGGAATTGATGGTCTGTCCCGAGGATGCTGCCCGCAACGATATTGCGGAT
GGTGATATCGTCCGTATCGGCAATACACGCGGCGAAGTGCGTCTCCATGCAAAGATCGTGGAGGGCGCACGGCCCGGTGT
GCTGATTGCCGAGGGTCTGTGGCCGAACAAGGCACATCTGGACGGCGAGGGCATCAATGTTCTGACCGGTGCCGATGCCG
TTGCGCCTTATGGTGGCGCCGCGTTTCACGACAACAAAGTATGGCTCCGCCGCGGCGACGAGGCCGGCGAGGCTTGA

Upstream 100 bases:

>100_bases
TCTCGCTTGGCGTTTATCGGGATGAATGGTCAGGATGAATATTCTTGATGGACGTCACTTCGGCCTTTTTGCCGCATCGT
TGATGCAATATACAAAAGAC

Downstream 100 bases:

>100_bases
TATTGCGCCGCCATACAGACAATCTGAAGCAACCCCACGACAGGCAAGGACATATCCGGTGACACAAACGCAGGCGAAAA
ACGTTCCGGCCGACATCGAT

Product: reductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MNVATPISAEKAASKAEVRIGHTVCPHDCPSACALEVDINADGRIGRVRGANANTYTAGVICAKVARYSERIYHPGRLLT
PKRRKGAKGAGDWQEISWEAALDEVADAFVKAEARHGAEAIWPYFYAGTMGQVQRDSIERLRHAKKYSGFFGSICTNMAW
TGYVMGTGALRGPDPREIGKADVVVIWGTNAVATQVNVMTHAVKARKERGAKIVVVDIYDNPTMKQADMRIVVRPGTDAA
LACAVMHIAFRDGYADRNYMARFADDPAGLEAHLKSKTPQWASEITGLSVEEIEAFARLVGTTKKTFFRLGYGFARQRNG
AVAMHAALSIATVLGSWQYEGGGAFHNNGDIFRLNKAELMGTAYADPDVRQLDQSQIGRVLTGDAEALRHGGPVTALLIQ
NTNPVNVAPEQRLVKQGFLRDDLFVAVHEQFMTETAEVADIVMPATMFLEHDDIYRAGGQNHILLGPKLVEPPETVRTNL
FVIEELAKRLGVDHMPGFGLSAREHVDRMLKVSGWGDFESLAEEKWIDAQPPFEMAHYLEGFGYGDGKFRFSPDWATGPS
PNKPPKNIGVMGPVSEFPKFPDQVDVIEVADADHPFRLATSPARNFLNSTFAETKTSVQKEGRPELMVCPEDAARNDIAD
GDIVRIGNTRGEVRLHAKIVEGARPGVLIAEGLWPNKAHLDGEGINVLTGADAVAPYGGAAFHDNKVWLRRGDEAGEA

Sequences:

>Translated_718_residues
MNVATPISAEKAASKAEVRIGHTVCPHDCPSACALEVDINADGRIGRVRGANANTYTAGVICAKVARYSERIYHPGRLLT
PKRRKGAKGAGDWQEISWEAALDEVADAFVKAEARHGAEAIWPYFYAGTMGQVQRDSIERLRHAKKYSGFFGSICTNMAW
TGYVMGTGALRGPDPREIGKADVVVIWGTNAVATQVNVMTHAVKARKERGAKIVVVDIYDNPTMKQADMRIVVRPGTDAA
LACAVMHIAFRDGYADRNYMARFADDPAGLEAHLKSKTPQWASEITGLSVEEIEAFARLVGTTKKTFFRLGYGFARQRNG
AVAMHAALSIATVLGSWQYEGGGAFHNNGDIFRLNKAELMGTAYADPDVRQLDQSQIGRVLTGDAEALRHGGPVTALLIQ
NTNPVNVAPEQRLVKQGFLRDDLFVAVHEQFMTETAEVADIVMPATMFLEHDDIYRAGGQNHILLGPKLVEPPETVRTNL
FVIEELAKRLGVDHMPGFGLSAREHVDRMLKVSGWGDFESLAEEKWIDAQPPFEMAHYLEGFGYGDGKFRFSPDWATGPS
PNKPPKNIGVMGPVSEFPKFPDQVDVIEVADADHPFRLATSPARNFLNSTFAETKTSVQKEGRPELMVCPEDAARNDIAD
GDIVRIGNTRGEVRLHAKIVEGARPGVLIAEGLWPNKAHLDGEGINVLTGADAVAPYGGAAFHDNKVWLRRGDEAGEA
>Mature_718_residues
MNVATPISAEKAASKAEVRIGHTVCPHDCPSACALEVDINADGRIGRVRGANANTYTAGVICAKVARYSERIYHPGRLLT
PKRRKGAKGAGDWQEISWEAALDEVADAFVKAEARHGAEAIWPYFYAGTMGQVQRDSIERLRHAKKYSGFFGSICTNMAW
TGYVMGTGALRGPDPREIGKADVVVIWGTNAVATQVNVMTHAVKARKERGAKIVVVDIYDNPTMKQADMRIVVRPGTDAA
LACAVMHIAFRDGYADRNYMARFADDPAGLEAHLKSKTPQWASEITGLSVEEIEAFARLVGTTKKTFFRLGYGFARQRNG
AVAMHAALSIATVLGSWQYEGGGAFHNNGDIFRLNKAELMGTAYADPDVRQLDQSQIGRVLTGDAEALRHGGPVTALLIQ
NTNPVNVAPEQRLVKQGFLRDDLFVAVHEQFMTETAEVADIVMPATMFLEHDDIYRAGGQNHILLGPKLVEPPETVRTNL
FVIEELAKRLGVDHMPGFGLSAREHVDRMLKVSGWGDFESLAEEKWIDAQPPFEMAHYLEGFGYGDGKFRFSPDWATGPS
PNKPPKNIGVMGPVSEFPKFPDQVDVIEVADADHPFRLATSPARNFLNSTFAETKTSVQKEGRPELMVCPEDAARNDIAD
GDIVRIGNTRGEVRLHAKIVEGARPGVLIAEGLWPNKAHLDGEGINVLTGADAVAPYGGAAFHDNKVWLRRGDEAGEA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=788, Percent_Identity=25, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI171474008, Length=773, Percent_Identity=24.3208279430789, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1787870, Length=553, Percent_Identity=27.124773960217, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI3868721, Length=509, Percent_Identity=25.3438113948919, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI87081994, Length=723, Percent_Identity=25.0345781466113, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI145693196, Length=688, Percent_Identity=25, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1787231, Length=682, Percent_Identity=22.8739002932551, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 78307; Mature: 78307

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVATPISAEKAASKAEVRIGHTVCPHDCPSACALEVDINADGRIGRVRGANANTYTAGV
CCCCCCCCHHHHCCCCEEEECCEECCCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHH
ICAKVARYSERIYHPGRLLTPKRRKGAKGAGDWQEISWEAALDEVADAFVKAEARHGAEA
HHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IWPYFYAGTMGQVQRDSIERLRHAKKYSGFFGSICTNMAWTGYVMGTGALRGPDPREIGK
HCHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCCCHHHCCC
ADVVVIWGTNAVATQVNVMTHAVKARKERGAKIVVVDIYDNPTMKQADMRIVVRPGTDAA
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCHH
LACAVMHIAFRDGYADRNYMARFADDPAGLEAHLKSKTPQWASEITGLSVEEIEAFARLV
HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHCCCCCHHHHHHCCCCHHHHHHHHHHH
GTTKKTFFRLGYGFARQRNGAVAMHAALSIATVLGSWQYEGGGAFHNNGDIFRLNKAELM
CCHHHHHHHHCCCHHHCCCCCEEHHHHHHHHHHHHCCEECCCCEECCCCCEEEEEHHHHE
GTAYADPDVRQLDQSQIGRVLTGDAEALRHGGPVTALLIQNTNPVNVAPEQRLVKQGFLR
EECCCCCHHHHHCHHHCCCEECCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCC
DDLFVAVHEQFMTETAEVADIVMPATMFLEHDDIYRAGGQNHILLGPKLVEPPETVRTNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCEEEECCCCCCCCHHHHHHH
FVIEELAKRLGVDHMPGFGLSAREHVDRMLKVSGWGDFESLAEEKWIDAQPPFEMAHYLE
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCHHHHHHHH
GFGYGDGKFRFSPDWATGPSPNKPPKNIGVMGPVSEFPKFPDQVDVIEVADADHPFRLAT
HCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEEC
SPARNFLNSTFAETKTSVQKEGRPELMVCPEDAARNDIADGDIVRIGNTRGEVRLHAKIV
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCEEEECCCCCCEEEEEEEE
EGARPGVLIAEGLWPNKAHLDGEGINVLTGADAVAPYGGAAFHDNKVWLRRGDEAGEA
CCCCCCEEEECCCCCCCCEECCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MNVATPISAEKAASKAEVRIGHTVCPHDCPSACALEVDINADGRIGRVRGANANTYTAGV
CCCCCCCCHHHHCCCCEEEECCEECCCCCCCCEEEEEEECCCCCEEEEECCCCCCCHHHH
ICAKVARYSERIYHPGRLLTPKRRKGAKGAGDWQEISWEAALDEVADAFVKAEARHGAEA
HHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IWPYFYAGTMGQVQRDSIERLRHAKKYSGFFGSICTNMAWTGYVMGTGALRGPDPREIGK
HCHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCCCCCCCHHHCCC
ADVVVIWGTNAVATQVNVMTHAVKARKERGAKIVVVDIYDNPTMKQADMRIVVRPGTDAA
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCHH
LACAVMHIAFRDGYADRNYMARFADDPAGLEAHLKSKTPQWASEITGLSVEEIEAFARLV
HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHCCCCCHHHHHHCCCCHHHHHHHHHHH
GTTKKTFFRLGYGFARQRNGAVAMHAALSIATVLGSWQYEGGGAFHNNGDIFRLNKAELM
CCHHHHHHHHCCCHHHCCCCCEEHHHHHHHHHHHHCCEECCCCEECCCCCEEEEEHHHHE
GTAYADPDVRQLDQSQIGRVLTGDAEALRHGGPVTALLIQNTNPVNVAPEQRLVKQGFLR
EECCCCCHHHHHCHHHCCCEECCCHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCC
DDLFVAVHEQFMTETAEVADIVMPATMFLEHDDIYRAGGQNHILLGPKLVEPPETVRTNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHCCCCCEEEECCCCCCCCHHHHHHH
FVIEELAKRLGVDHMPGFGLSAREHVDRMLKVSGWGDFESLAEEKWIDAQPPFEMAHYLE
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEECCCCCHHHHHHHCCCCCCCCHHHHHHHH
GFGYGDGKFRFSPDWATGPSPNKPPKNIGVMGPVSEFPKFPDQVDVIEVADADHPFRLAT
HCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEEC
SPARNFLNSTFAETKTSVQKEGRPELMVCPEDAARNDIADGDIVRIGNTRGEVRLHAKIV
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCCCCEEEECCCCCCEEEEEEEE
EGARPGVLIAEGLWPNKAHLDGEGINVLTGADAVAPYGGAAFHDNKVWLRRGDEAGEA
CCCCCCEEEECCCCCCCCEECCCCEEEEECCCCCCCCCCCEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]